BRDT (NM_001242808) Human Tagged ORF Clone

CAT#: RC235008

BRDT (Myc-DDK tagged) - Homo sapiens bromodomain, testis-specific (BRDT), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRDT
• Synonyms: BRD6; CT9; SPGF21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235008 representing NM_001242808
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTGCCAAGTCGACAAACAGCTATTATTGTTAACCCTCCTCCACCAGAATATATAAATACTAAGA
  AAAATGGGCGATTGACAAATCAACTTCAGTATCTACAAAAAGTTGTCCTAAAGGATTTATGGAAGCATAG
  TTTTTCATGGCCCTTTCAACGTCCTGTGGATGCTGTGAAACTACAGTTGCCTCCTGGAGATGACATTGTT
  CTTATGGCACAAGCTCTAGAGAAGCTGTTTATGCAGAAATTATCTCAGATGCCACAAGAAGAGCAAGTTG
  TGGGTGTTAAGGAAAGAATCAAGAAAGGCACTCAACAGAATATAGCTGTTTCTTCTGCTAAAGAAAAATC
  ATCACCCAGCGCAACAGAAAAAGTATTTAAGCAGCAAGAAATTCCTTCTGTATTTCCTAAGACATCTATT
  TCTCCCTTGAACGTGGTACAGGGAGCTTCAGTCAACTCCAGTTCACAAACTGCGGCCCAAGTTACAAAAG
  GTGTGAAGAGGAAAGCAGATACAACAACTCCTGCAACTTCAGCAGTTAAAGCAAGTAGTGAATTTTCTCC
  AACATTCACAGAAAAATCAGTGGCACTGCCACCTATAAAAGAAAATATGCCAAAGAATGTTTTGCCAGAT
  TCTCAGCAACAATATAATGTTGTGAAGACTGTTAAAGTAACTGAACAATTAAGGCACTGTAGTGAGATTC
  TTAAAGAAATGCTTGCAAAGAAACATTTTTCATATGCATGGCCCTTTTATAATCCTGTTGACGTTAATGC
  TTTGGGACTCCATAACTACTATGACGTTGTCAAAAATCCGATGGATCTTGGAACTATTAAGGAGAAAATG
  GATAACCAAGAATATAAGGATGCATACAAATTTGCGGCAGATGTTAGATTAATGTTCATGAATTGCTACA
  AGTACAATCCTCCAGATCACGAAGTTGTGACAATGGCAAGAATGCTTCAGGATGTTTTCGAAACGCATTT
  TTCAAAGATCCCGATTGAACCTGTTGAGAGTATGCCTTTATGTTACATCAAAACAGATATCACAGAAACC
  ACTGGTAGAGAGAACACTAATGAAGCCTCCTCTGAAGGGAACTCTTCTGATGATTCTGAAGATGAGCGAG
  TTAAGCGTCTTGCAAAGCTTCAGGAGCAGCTTAAAGCTGTACATCAACAGCTCCAGGTTTTGTCCCAAGT
  ACCTTTCCGTAAGCTAAATAAAAAGAAAGAGAAGTCTAAAAAGGAAAAGAAAAAAGAAAAGGTTAATAAC
  AGCAATGAAAATCCAAGAAAAATGTGTGAGCAAATGAGGCTAAAGGAAAAGTCCAAGAGAAATCAGCCAA
  AGAAAAGGAAACAACAGTTCATTGGTCTAAAATCTGAAGATGAAGATAATGCTAAACCTATGAACTATGA
  TGAGAAAAGGCAGTTAAGTCTGAATATAAACAAACTCCCTGGAGATAAACTTGGGCGAGTAGTTCACATA
  ATACAATCAAGAGAGCCTTCTCTGAGCAATTCCAATCCTGATGAGATAGAGATAGACTTTGAAACACTGA
  AAGCATCAACACTAAGAGAATTAGAAAAATATGTTTCGGCATGTCTAAGAAAGAGACCATTAAAACCTCC
  TGCTAAGAAAATAATGATGTCCAAAGAAGAACTTCACTCACAGAAAAAACAGGAATTGGAAAAGCGGTTA
  CTGGATGTTAATAATCAGTTAAATTCTAGAAAACGTCAAACAAAATCTGATAAAACGCAACCATCCAAAG
  CTGTTGAAAATGTTTCCCGACTGAGTGAGAGCAGCAGCAGCAGCAGCAGCTCATCAGAGTCTGAAAGTAG
  CAGCAGTGACTTAAGCTCTTCAGACAGCAGTGATTCTGAATCAGAAATGTTCCCTAAGTTTACAGAAGTA
  AAACCAAATGATTCTCCTTCTAAAGAGAATGTAAAGAAAATGAAGAATGAATGCATACTGCCTGAAGGAA
  GAACAGGCGTCACACAGATAGGATATTGTGTGCAAGACACAACCTCTGCCAATACTACCCTTGTTCATCA
  GACCACACCTTCACATGTAATGCCACCAAATCACCACCAATTAGCATTTAATTATCAAGAATTAGAACAT
  TTACAGACTGTGAAAAACATTTCACCTTTACAAATTCTGCCTCCCTCAGGTGATTCTGAACAGCTCTCAA
  ATGGCATAACTGTGATGCATCCATCTGGTGATAGTGACACAACGATGTTAGAATCTGAATGTCAAGCTCC
  TGTACAGAAGGATATAAAGATTAAGAATGCAGATTCATGGAAAAGTTTAGGCAAACCAGTGAAACCATCA
  GGTGTAATGAAATCCTCAGATGAGCTCTTCAACCAATTTAGAAAAGCAGCCATAGAAAAGGAAGTAAAAG
  CTCGGACACAGGAACTCATACGGAAGCATTTGGAACAAAATACAAAGGAACTAAAAGCATCTCAAGAAAA
  TCAGAGGGATCTTGGGAATGGATTGACTGTAGAATCTTTTTCAAATAAAATACAAAACAAGTGCTCTGGA
  GAAGAGCAGAAAGAACATCAGCAGTCATCAGAAGCTCAAGATAAATCCAAACTCTGGCTTCTCAAAGACC
  GTGATTTAGCAAGGCAGAAAGAACAAGAGAGGAGGAGGAGAGAAGCAATGGTGGGTACCATTGATATGAC
  CCTTCAAAGTGACATTATGACAATGTTTGAAAACAACTTTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235008 representing NM_001242808
  Red=Cloning site Green=Tags(s)
  
  MSLPSRQTAIIVNPPPPEYINTKKNGRLTNQLQYLQKVVLKDLWKHSFSWPFQRPVDAVKLQLPPGDDIV
  LMAQALEKLFMQKLSQMPQEEQVVGVKERIKKGTQQNIAVSSAKEKSSPSATEKVFKQQEIPSVFPKTSI
  SPLNVVQGASVNSSSQTAAQVTKGVKRKADTTTPATSAVKASSEFSPTFTEKSVALPPIKENMPKNVLPD
  SQQQYNVVKTVKVTEQLRHCSEILKEMLAKKHFSYAWPFYNPVDVNALGLHNYYDVVKNPMDLGTIKEKM
  DNQEYKDAYKFAADVRLMFMNCYKYNPPDHEVVTMARMLQDVFETHFSKIPIEPVESMPLCYIKTDITET
  TGRENTNEASSEGNSSDDSEDERVKRLAKLQEQLKAVHQQLQVLSQVPFRKLNKKKEKSKKEKKKEKVNN
  SNENPRKMCEQMRLKEKSKRNQPKKRKQQFIGLKSEDEDNAKPMNYDEKRQLSLNINKLPGDKLGRVVHI
  IQSREPSLSNSNPDEIEIDFETLKASTLRELEKYVSACLRKRPLKPPAKKIMMSKEELHSQKKQELEKRL
  LDVNNQLNSRKRQTKSDKTQPSKAVENVSRLSESSSSSSSSSESESSSSDLSSSDSSDSESEMFPKFTEV
  KPNDSPSKENVKKMKNECILPEGRTGVTQIGYCVQDTTSANTTLVHQTTPSHVMPPNHHQLAFNYQELEH
  LQTVKNISPLQILPPSGDSEQLSNGITVMHPSGDSDTTMLESECQAPVQKDIKIKNADSWKSLGKPVKPS
  GVMKSSDELFNQFRKAAIEKEVKARTQELIRKHLEQNTKELKASQENQRDLGNGLTVESFSNKIQNKCSG
  EEQKEHQQSSEAQDKSKLWLLKDRDLARQKEQERRRREAMVGTIDMTLQSDIMTMFENNFD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242808
• ORF Size: 2703 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242808.1, NM_001242808.2, NP_001229737.1
• RefSeq Size: 3223 bp
• RefSeq ORF: 2706 bp
• Locus ID: 676
• Cytogenetics: 1p22.1
• Protein Families: Protein Kinase, Transcription Factors
• MW: 102.8 kDa
• Gene Summary: 'BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jun 2011]'