ITCH (NM_001257137) Human Tagged ORF Clone

CAT#: RC235014

ITCH (Myc-DDK tagged) - Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ITCH
• Synonyms: ADMFD; AIF4; AIP4; NAPP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235014 representing NM_001257137
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGACAGTGGATCACAACTTGGTTCAATGGGTAGCCTCACCATGAAATCACAGCTTCAGATCACTG
  TCATCTCAGCAAAACTTAAGGAAAATAAGAAGAATTGGTTTGGACCAAGTCCTTACGTAGAGGTCACAGT
  AGATGGACAGTCAAAGAAGACAGAAAAATGCAACAACACAAACAGTCCCAAGTGGAAGCAACCCCTTACA
  GTTATCGTTACCCCTGTGAGTAAATTACATTTTCGTGTGTGGAGTCACCAGACACTGAAATCTGATGTTT
  TGTTGGGAACTGCTGCATTAGATATTTATGAAACATTAAAGTCAAACAATATGAAACTTGAAGAAGTAGT
  TGTGACTTTGCAGCTTGGAGGTGACAAAGAGCCAACAGAGACAATAGGAGACTTGTCAATTTGTCTTGAT
  GGGCTACAGTTAGAGTCTGAAGTTGTTACCAATGGTGAAACTACATGTTCAGAAAATGGGGTTTCGCTCT
  GTTTGCCCAGGCTGGAGTGCAATAGTGCGATCTCGGCTCACTGCAACCTCTGCCTCCCGGGTTTAAGTGA
  TTCTCCTATCTCAGCCTCCCGAGTAGCTGGGTTTACAGGTGCTTCTCAGAATGATGATGGCTCCAGATCC
  AAGGATGAAACAAGAGTGAGCACAAATGGATCAGATGACCCTGAAGATGCAGGAGCTGGTGAAAATAGGA
  GAGTCAGTGGGAATAATTCTCCATCACTCTCAAATGGTGGTTTTAAACCTTCTAGACCTCCAAGACCTTC
  ACGACCACCACCACCCACCCCACGTAGACCAGCATCTGTCAATGGTTCACCATCTGCCACTTCTGAAAGT
  GATGGGTCTAGTACAGGCTCTCTGCCGCCGACAAATACAAATACAAATACATCTGAAGGAGCAACATCTG
  GATTAATAATTCCTCTTACTATATCTGGAGGCTCAGGCCCTAGGCCATTAAATCCTGTAACTCAAGCTCC
  CTTGCCACCTGGTTGGGAGCAGAGAGTGGACCAGCACGGGCGAGTTTACTATGTAGATCATGTTGAGAAA
  AGAACAACATGGGATAGACCAGAACCTCTACCTCCTGGCTGGGAACGGCGGGTTGACAACATGGGACGTA
  TTTATTATGTTGACCATTTCACAAGAACAACAACGTGGCAGAGGCCAACACTGGAATCCGTCCGGAACTA
  TGAACAATGGCAGCTACAGCGTAGTCAGCTTCAAGGAGCAATGCAGCAGTTTAACCAGAGATTCATTTAT
  GGGAATCAAGATTTATTTGCTACATCACAAAGTAAAGAATTTGATCCTCTTGGTCCATTGCCACCTGGAT
  GGGAGAAGAGAACAGACAGCAATGGCAGAGTATATTTCGTCAACCACAACACACGAATTACACAATGGGA
  AGACCCCAGAAGTCAAGGTCAATTAAATGAAAAGCCCTTACCTGAAGGTTGGGAAATGAGATTCACAGTG
  GATGGAATTCCATATTTTGTGGACCACAATAGAAGAACTACCACCTATATAGATCCCCGCACAGGAAAAT
  CTGCCCTAGACAATGGACCTCAGATAGCCTATGTTCGGGACTTCAAAGCAAAGGTTCAGTATTTCCGGTT
  CTGGTGTCAGCAACTGGCCATGCCACAGCACATAAAGATTACAGTGACAAGAAAAACATTGTTTGAGGAT
  TCCTTTCAACAGATAATGAGCTTCAGTCCCCAAGATCTGCGAAGACGTTTGTGGGTGATTTTTCCAGGAG
  AAGAAGGTTTAGATTATGGAGGTGTAGCAAGAGAATGGTTCTTTCTTTTGTCACATGAAGTGTTGAACCC
  AATGTATTGCCTGTTTGAATATGCAGGGAAGGATAACTACTGCTTGCAGATAAACCCCGCTTCTTACATC
  AATCCAGATCACCTGAAATATTTTCGTTTTATTGGCAGATTTATTGCCATGGCTCTGTTCCATGGGAAAT
  TCATAGACACGGGTTTTTCTTTACCATTCTATAAGCGTATCTTGAACAAACCAGTTGGACTCAAGGATTT
  AGAATCTATTGATCCAGAATTTTACAATTCTCTCATCTGGGTTAAGGAAAACAATATTGAGGAATGTGAT
  TTGGAAATGTACTTCTCCGTTGACAAAGAAATTCTAGGTGAAATTAAGAGTCATGATCTGAAACCTAATG
  GTGGCAATATTCTTGTAACAGAAGAAAATAAAGAGGAATACATCAGAATGGTAGCTGAGTGGAGGTTGTC
  TCGAGGTGTTGAAGAACAGACACAAGCTTTCTTTGAAGGCTTTAATGAAATTCTTCCCCAGCAATATTTG
  CAATACTTTGATGCAAAGGAATTAGAGGTCCTTTTATGTGGAATGCAAGAGATTGATTTGAATGACTGGC
  AAAGACATGCCATCTACCGTCATTATGCAAGGACCAGCAAACAAATCATGTGGTTTTGGCAGTTTGTTAA
  AGAAATTGATAATGAGAAGAGAATGAGACTTCTGCAGTTTGTTACTGGAACCTGCCGATTGCCAGTAGGA
  GGATTTGCTGATCTCATGGGGAGCAATGGACCACAGAAATTCTGCATTGAAAAAGTTGGGAAAGAAAATT
  GGCTACCCAGAAGTCATACCTGTTTTAATCGCCTGGACCTGCCACCATACAAGAGCTATGAGCAACTGAA
  GGAAAAGCTGTTGTTTGCCATAGAAGAAACAGAAGGATTTGGACAAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235014 representing NM_001257137
  Red=Cloning site Green=Tags(s)
  
  MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLT
  VIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLD
  GLQLESEVVTNGETTCSENGVSLCLPRLECNSAISAHCNLCLPGLSDSPISASRVAGFTGASQNDDGSRS
  KDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSES
  DGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEK
  RTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIY
  GNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTV
  DGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFED
  SFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYI
  NPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECD
  LEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYL
  QYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVG
  GFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001257137
• ORF Size: 2709 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001257137.1, NM_001257137.2, NP_001244066.1
• RefSeq Size: 6535
• RefSeq ORF: 2712
• Locus ID: 83737
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Endocytosis, Ubiquitin mediated proteolysis
• MW: 103.3 kDa
• Gene Summary: This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]