BRDT (NM_001242806) Human Tagged ORF Clone

CAT#: RC235075

BRDT (Myc-DDK tagged) - Homo sapiens bromodomain, testis-specific (BRDT), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRDT
• Synonyms: BRD6; CT9; SPGF21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235075 representing NM_001242806
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTGCCAAGTCGACAAACAGCTATTATTGTTAACCCTCCTCCACCAGAATATATAAATACTAAGA
  AAAATGGGCGATTGACAAATCAACTTCAGTATCTACAAAAAGTTGTCCTAAAGGATTTATGGAAGCATAG
  TTTTTCATGGCCCTTTCAACGTCCTGTGGATGCTGTGAAACTACAGTTGCCTGATTATTATACCATTATA
  AAAAACCCAATGGATTTAAATACAATTAAGAAGCGCTTGGAGAATAAATATTATGCGAAGGCTTCAGAAT
  GTATAGAAGACTTCAATACAATGTTCTCAAATTGTTATTTATATAACAAGCCTGGAGATGACATTGTTCT
  TATGGCACAAGCTCTAGAGAAGCTGTTTATGCAGAAATTATCTCAGATGCCACAAGAAGAGCAAGTTGTG
  GGTGTTAAGGAAAGAATCAAGAAAGGTAAGGCAGGAGGCACTCAACAGAATATAGCTGTTTCTTCTGCTA
  AAGAAAAATCATCACCCAGCGCAACAGAAAAAGTATTTAAGCAGCAAGAAATTCCTTCTGTATTTCCTAA
  GACATCTATTTCTCCCTTGAACGTGGTACAGGGAGCTTCAGTCAACTCCAGTTCACAAACTGCGGCCCAA
  GTTACAAAAGGTGTGAAGAGGAAAGCAGATACAACAACTCCTGCAACTTCAGCAGTTAAAGCAAGTAGTG
  AATTTTCTCCAACATTCACAGAAAAATCAGTGGCACTGCCACCTATAAAAGAAAATATGCCAAAGAATGT
  TTTGCCAGATTCTCAGCAACAATATAATGTTGTGAAGACTGTTAAAGTAACTGAACAATTAAGGCACTGT
  AGTGAGATTCTTAAAGAAATGCTTGCAAAGAAACATTTTTCATATGCATGGCCCTTTTATAATCCTGTTG
  ACGTTAATGCTTTGGGACTCCATAACTACTATGACGTTGTCAAAAATCCGATGGATCTTGGAACTATTAA
  GGAGAAAATGGATAACCAAGAATATAAGGATGCATACAAATTTGCGGCAGATGTTAGATTAATGTTCATG
  AATTGCTACAAGTACAATCCTCCAGATCACGAAGTTGTGACAATGGCAAGAATGCTTCAGGATGTTTTCG
  AAACGCATTTTTCAAAGATCCCGATTGAACCTGTTGAGAGTATGCCTTTATGTTACATCAAAACAGATAT
  CACAGAAACCACTGGTAGAGAGAACACTAATGAAGCCTCCTCTGAAGGGAACTCTTCTGATGATTCTGAA
  GATGAGCGAGTTAAGCGTCTTGCAAAGCTTCAGGAGCAGCTTAAAGCTGTACATCAACAGCTCCAGGTTT
  TGTCCCAAGTACCTTTCCGTAAGCTAAATAAAAAGAAAGAGAAGTCTAAAAAGGAAAAGAAAAAAGAAAA
  GGTTAATAACAGCAATGAAAATCCAAGAAAAATGTGTGAGCAAATGAGGCTAAAGGAAAAGTCCAAGAGA
  AATCAGCCAAAGAAAAGGAAACAACAGTTCATTGGTCTAAAATCTGAAGATGAAGATAATGCTAAACCTA
  TGAACTATGATGAGAAAAGGCAGTTAAGTCTGAATATAAACAAACTCCCTGGAGATAAACTTGGGCGAGT
  AGTTCACATAATACAATCAAGAGAGCCTTCTCTGAGCAATTCCAATCCTGATGAGATAGAGATAGACTTT
  GAAACACTGAAAGCATCAACACTAAGAGAATTAGAAAAATATGTTTCGGCATGTCTAAGAAAGAGACCAT
  TAAAACCTCCTGCTAAGAAAATAATGATGTCCAAAGAAGAACTTCACTCACAGAAAAAACAGGAATTGGA
  AAAGCGGTTACTGGATGTTAATAATCAGTTAAATTCTAGAAAACGTCAAACAAAATCTGATAAAACGCAA
  CCATCCAAAGCTGTTGAAAATGTTTCCCGACTGAGTGAGAGCAGCAGCAGCAGCAGCAGCTCATCAGAGT
  CTGAAAGTAGCAGCAGTGACTTAAGCTCTTCAGACAGCAGTGATTCTGAATCAGAAATGTTCCCTAAGTT
  TACAGAAGTAAAACCAAATGATTCTCCTTCTAAAGAGAATGTAAAGAAAATGAAGAATGAATGCATACTG
  CCTGAAGGAAGAACAGGCGTCACACAGATAGGATATTGTGTGCAAGACACAACCTCTGCCAATACTACCC
  TTGTTCATCAGACCACACCTTCACATGTAATGCCACCAAATCACCACCAATTAGCATTTAATTATCAAGA
  ATTAGAACATTTACAGACTGTGAAAAACATTTCACCTTTACAAATTCTGCCTCCCTCAGGTGATTCTGAA
  CAGCTCTCAAATGGCATAACTGTGATGCATCCATCTGGTGATAGTGACACAACGATGTTAGAATCTGAAT
  GTCAAGCTCCTGTACAGAAGGATATAAAGATTAAGAATGCAGATTCATGGAAAAGTTTAGGCAAACCAGT
  GAAACCATCAGGTGTAATGAAATCCTCAGATGAGCTCTTCAACCAATTTAGAAAAGCAGCCATAGAAAAG
  GAAGTAAAAGCTCGGACACAGGAACTCATACGGAAGCATTTGGAACAAAATACAAAGGAACTAAAAGCAT
  CTCAAGAAAATCAGAGGGATCTTGGGAATGGATTGACTGTAGAATCTTTTTCAAATAAAATACAAAACAA
  GTGCTCTGGAGAAGAGCAGAAAGAACATCAGCAGTCATCAGAAGCTCAAGATAAATCCAAACTCTGGCTT
  CTCAAAGACCGTGATTTAGCAAGGCAGAAAGAACAAGAGAGGAGGAGGAGAGAAGCAATGGTGGGTACCA
  TTGATATGACCCTTCAAAGTGACATTATGACAATGTTTGAAAACAACTTTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235075 representing NM_001242806
  Red=Cloning site Green=Tags(s)
  
  MSLPSRQTAIIVNPPPPEYINTKKNGRLTNQLQYLQKVVLKDLWKHSFSWPFQRPVDAVKLQLPDYYTII
  KNPMDLNTIKKRLENKYYAKASECIEDFNTMFSNCYLYNKPGDDIVLMAQALEKLFMQKLSQMPQEEQVV
  GVKERIKKGKAGGTQQNIAVSSAKEKSSPSATEKVFKQQEIPSVFPKTSISPLNVVQGASVNSSSQTAAQ
  VTKGVKRKADTTTPATSAVKASSEFSPTFTEKSVALPPIKENMPKNVLPDSQQQYNVVKTVKVTEQLRHC
  SEILKEMLAKKHFSYAWPFYNPVDVNALGLHNYYDVVKNPMDLGTIKEKMDNQEYKDAYKFAADVRLMFM
  NCYKYNPPDHEVVTMARMLQDVFETHFSKIPIEPVESMPLCYIKTDITETTGRENTNEASSEGNSSDDSE
  DERVKRLAKLQEQLKAVHQQLQVLSQVPFRKLNKKKEKSKKEKKKEKVNNSNENPRKMCEQMRLKEKSKR
  NQPKKRKQQFIGLKSEDEDNAKPMNYDEKRQLSLNINKLPGDKLGRVVHIIQSREPSLSNSNPDEIEIDF
  ETLKASTLRELEKYVSACLRKRPLKPPAKKIMMSKEELHSQKKQELEKRLLDVNNQLNSRKRQTKSDKTQ
  PSKAVENVSRLSESSSSSSSSSESESSSSDLSSSDSSDSESEMFPKFTEVKPNDSPSKENVKKMKNECIL
  PEGRTGVTQIGYCVQDTTSANTTLVHQTTPSHVMPPNHHQLAFNYQELEHLQTVKNISPLQILPPSGDSE
  QLSNGITVMHPSGDSDTTMLESECQAPVQKDIKIKNADSWKSLGKPVKPSGVMKSSDELFNQFRKAAIEK
  EVKARTQELIRKHLEQNTKELKASQENQRDLGNGLTVESFSNKIQNKCSGEEQKEHQQSSEAQDKSKLWL
  LKDRDLARQKEQERRRREAMVGTIDMTLQSDIMTMFENNFD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242806
• ORF Size: 2853 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242806.1, NM_001242806.2, NP_001229735.1
• RefSeq Size: 3373 bp
• RefSeq ORF: 2856 bp
• Locus ID: 676
• Cytogenetics: 1p22.1
• Protein Families: Protein Kinase, Transcription Factors
• MW: 108.7 kDa
• Gene Summary: 'BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jun 2011]'