EFTUD2 (NM_001258354) Human Tagged ORF Clone

CAT#: RC235082

EFTUD2 (Myc-DDK tagged) - Homo sapiens elongation factor Tu GTP binding domain containing 2 (EFTUD2), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EFTUD2
• Synonyms: MFDGA; MFDM; SNRNP116; Snrp116; Snu114; U5-116KD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235082 representing NM_001258354
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATACCGACTTATATGATGAGTTTGGGAATTATATTGGACCAGAGCTTGATTCTGATGAAGATGATG
  ATGAATTGGGTAGAGAGACCAAAGATCTTGATGAGATGGATGATGATGACGACGACGATGACGTAGGAGA
  TCATGACGATGACCACCCTGGGATGGAGGTGGTGCTGCATGAGGACAAGAAGTACTACCCAACAGCCGAG
  GAGGTGTATGGTCCTGAGGTGGAGACCATAGTTCAAGAGGAAGACACTCAGCCTCTCACAGAACCCATTA
  TTAAGCCAGTGAAAACCAAGAAATTCACTCTGATGGAGCAGACATTACCTGTTACGGTGTATGAGATGGA
  TTTCTTGGCGGATCTGATGGATAACTCAGAGCTCATCAGAAATGTGACCCTTTGTGGACATCTCCACCAT
  GGCAAGACTCACCCGGAAATCAGAAAGCGCTATGACCAAGATCTGTGCTATACTGACATCCTCTTCACAG
  AGCAAGAGAGAGGTGTAGGCATCAAAAGCACTCCTGTGACAGTGGTCTTGCCAGACACCAAAGGAAAATC
  TTATCTCTTCAATATCATGGACACTCCAGGACATGTGAATTTCTCTGATGAGGTCACAGCTGGCTTGCGC
  ATCTCAGATGGAGTGGTCCTTTTCATTGATGCTGCTGAGGGGGTGATGCTGAACACAGAGCGGCTGATCA
  AGCATGCGGTGCAGGAGAGGCTGGCAGTCACTGTGTGCATCAACAAGATTGACCGGCTGATCCTGGAGCT
  GAAGCTGCCTCCAACTGATGCTTATTACAAGCTGCGCCACATTGTGGATGAGGTCAATGGATTAATAAGC
  ATGTATTCCACTGATGAGAACCTGATCCTTTCCCCACTCCTGGGTAACGTCTGCTTCTCCAGCTCCCAGT
  ACAGCATCTGCTTCACGCTGGGCTCCTTTGCCAAGATCTATGCCGACACCTTTGGTGACATTAATTACCA
  AGAATTTGCTAAAAGACTCTGGGGTGACATCTACTTCAACCCTAAGACGCGAAAGTTCACCAAAAAGGCC
  CCAACTAGCAGCTCCCAGAGAAGTTTCGTGGAGTTTATCTTGGAGCCTCTTTATAAGATCCTCGCCCAGG
  TTGTAGGTGACGTGGACACCAGCCTCCCACGGACCCTAGACGAGCTTGGCATCCACCTGACGAAGGAGGA
  GCTGAAGCTGAACATCCGCCCCTTGCTCAGGCTGGTCTGCAAAAAGTTCTTTGGCGAGTTCACAGGCTTT
  GTGGACATGTGTGTGCAGCATATCCCTTCTCCAAAGGTGGGCGCCAAGCCCAAGATTGAGCACACCTACA
  CCGGTGGTGTGGACTCCGACCTCGGCGAGGCTATGAGTGACTGTGACCCTGATGGCCCCCTGATGTGCCA
  CACTACTAAGATGTACAGCACAGATGATGGAGTCCAGTTTCACGCCTTTGGCCGGGTGCTGAGTGGCACC
  ATTCATGCTGGGCAGCCTGTGAAGGTACTGGGGGAGAACTACACCCTGGAGGATGAGGAAGACTCCCAGA
  TATGCACCGTGGGCCGCCTTTGGATCTCTGTGGCCAGGTACCACATCGAGGTGAACCGTGTTCCTGCTGG
  CAACTGGGTTCTGATTGAAGGTGTTGATCAACCAATTGTGAAGACAGCAACCATAACCGAACCCCGAGGC
  AATGAGGAGGCTCAGATTTTCCGACCCTTGAAGTTCAATACCACATCTGTTATCAAGATTGCTGTGGAGC
  CAGTCAACCCCTCAGAGCTGCCCAAGATGCTTGATGGCCTGCGCAAGGTCAACAAGAGCTATCCATCCCT
  CACCACCAAGGTGGAGGAGTCTGGCGAGCATGTGATCCTGGGCACTGGGGAGCTCTACCTGGACTGTGTG
  ATGCATGATTTGCGGAAGATGTACTCAGAGATAGACATCAAGGTGGCTGACCCAGTTGTCACGTTTTGTG
  AGACGGTGGTGGAAACATCCTCCCTCAAGTGCTTTGCTGAAACGCCTAATAAGAAGAACAAGATCACCAT
  GATTGCTGAGCCTCTTGAGAAGGGCCTGGCAGAGGACATAGAGAATGAGGTGGTCCAGATTACGTGGAAC
  AGGAAGAAGCTGGGAGAGTTCTTCCAGACCAAGTACGATTGGGATCTGCTGGCTGCCCGTTCCATCTGGG
  CTTTTGGCCCTGATGCGACTGGCCCCAACATTCTGGTGGATGATACTCTGCCCTCTGAGGTGGACAAGGC
  TCTTCTTGGTTCAGTGAAGGACAGCATCGTTCAAGGTTTCCAGTGGGGAACCAGGGAGGGCCCCCTCTGT
  GATGAATTGATTCGGAATGTCAAGTTTAAGATCCTGGATGCGGTGGTTGCCCAGGAGCCCCTGCACCGGG
  GCGGGGGCCAGATCATCCCCACAGCCAGGAGAGTCGTCTACTCTGCCTTCCTCATGGCTACTCCTCGTCT
  GATGGAGCCTTACTACTTTGTAGAGGTCCAGGCCCCTGCAGATTGCGTCTCTGCAGTTTATACCGTCCTG
  GCCAGGCGCAGGGGGCACGTGACTCAGGATGCACCCATCCCAGGCTCCCCTCTGTACACCATCAAAGCTT
  TTATCCCGGCCATCGACTCTTTTGGCTTTGAGACTGATCTCCGGACTCACACCCAGGGACAAGCCTTTTC
  TCTGTCTGTCTTCCACCACTGGCAGATTGTGCCTGGTGATCCCCTGGACAAGAGCATTGTCATCCGCCCC
  TTGGAGCCACAGCCAGCTCCTCACCTGGCCCGGGAATTCATGATCAAAACCCGCCGTAGGAAGGGCCTCA
  GTGAAGATGTGAGCATCAGCAAATTCTTCGATGATCCTATGTTGCTGGAACTTGCCAAACAGGATGTTGT
  GCTCAATTACCCCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235082 representing NM_001258354
  Red=Cloning site Green=Tags(s)
  
  MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLHEDKKYYPTAE
  EVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLADLMDNSELIRNVTLCGHLHH
  GKTHPEIRKRYDQDLCYTDILFTEQERGVGIKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLR
  ISDGVVLFIDAAEGVMLNTERLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLIS
  MYSTDENLILSPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKA
  PTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVCKKFFGEFTGF
  VDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTKMYSTDDGVQFHAFGRVLSGT
  IHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIEVNRVPAGNWVLIEGVDQPIVKTATITEPRG
  NEEAQIFRPLKFNTTSVIKIAVEPVNPSELPKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCV
  MHDLRKMYSEIDIKVADPVVTFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWN
  RKKLGEFFQTKYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLC
  DELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPADCVSAVYTVL
  ARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSVFHHWQIVPGDPLDKSIVIRP
  LEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLELAKQDVVLNYPM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258354
• ORF Size: 2886 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001258354.1, NP_001245283.1
• RefSeq Size: 4137
• RefSeq ORF: 2889
• Locus ID: 9343
• Protein Pathways: Spliceosome
• MW: 108.7 kDa
• Gene Summary: This gene encodes a GTPase which is a component of the spliceosome complex which processes precursor mRNAs to produce mature mRNAs. Mutations in this gene are associated with mandibulofacial dysostosis with microcephaly. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]