PSD (NM_001270965) Human Tagged ORF Clone

CAT#: RC235126

PSD (Myc-DDK tagged) - Homo sapiens pleckstrin and Sec7 domain containing (PSD), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PSD
• Synonyms: EFA6; EFA6A; PSD1; TYL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235126 representing NM_001270965
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGGGTGCCATGCGCTTCTGCTCGGAAGGCGACTGTGCCATCTCCCCACCACGATGCCCACGCC
  GCTGGCTCCCCGAAGGCCCGGTGCCCCAGAGCCCCCCAGCCAGCATGTATGGCAGCACAGGCTCCTTGCT
  ACGGCGAGTGGCAGGTCCAGGTCCTCGAGGCCGGGAACTGGGACGTGTGACAGCACCCTGTACACCTCTG
  CGTGGCCCCCCCTCACCCCGTGTTGCTCCCTCACCCTGGGCACCCTCTTCACCCACTGGGCAGCCCCCAC
  CCGGGGCCCAGAGCTCTGTGGTCATCTTCCGCTTTGTGGAGAAGGCCAGTGTGAGGCCACTGAATGGGCT
  ACCTGCTCCAGGGGGCTTGAGTCGGAGCTGGGATCTGGGTGGGGTTTCTCCTCCCAGGCCCACCCCAGCC
  CTTGGGCCTGGCTCCAACCGGAAGTTACGGCTGGAAGCATCCACATCAGACCCACTCCCCGCCAGAGGAG
  GCTCGGCCCTACCTGGCAGCCGGAACCTTGTACATGGGCCGCCAGCCCCACCCCAGGTTGGAGCAGATGG
  CCTTTACTCCTCTCTCCCCAATGGGCTGGGGGGCCCCCCTGAGCGCCTGGCCACACTCTTCGGAGGACCT
  GCTGACACTGGATTCCTGAACCAGGGGGATACCTGGTCCTCCCCCCGGGAAGTCTCCTCTCATGCCCAGA
  GAATCGCTAGAGCCAAATGGGAATTCTTCTATGGCTCCTTGGACCCCCCCAGCTCAGGTGCTAAGCCCCC
  AGAGCAGGCCCCCCCATCTCCACCTGGGGTGGGCTCAAGGCAGGGCTCTGGGGTGGCTGTGGGGCGAGCA
  GCCAAGTACTCCGAGACAGACCTGGACACGGTGCCCCTGAGGTGCTACCGCGAGACTGACATCGATGAGG
  TGCTGGCTGAGCGGGAGGAGGCCGACTCGGCCATCGAAAGTCAGCCCAGCTCTGAGGGCCCACCAGGCAC
  TGCCTACCCACCTGCCCCACGGCCCGGCCCACTCCCTGGCCCTCATCCCAGCCTCGGCAGTGGCAATGAG
  GATGAGGACGACGATGAGGCAGGTGGGGAAGAAGATGTGGACGACGAGGTGTTTGAGGCCTCTGAAGGGG
  CCCGGCCAGGGAGCCGGATGCCTCTCAAGTCACCTGTGCCCTTTCTACCTGGGACGAGCCCCTCGGCTGA
  TGGGCCTGACTCTTTCAGTTGTGTGTTCGAAGCCATCCTGGAGTCACACCGGGCCAAAGGCACCTCCTAT
  ACCAGCCTCGCCTCGCTGGAGGCCTTGGCCTCACCTGGCCCAACCCAGAGCCCCTTCTTCACCTTTGAGC
  TGCCTCCCCAACCCCCTGCACCCCGGCCCGACCCACCAGCTCCCGCCCCACTTGCCCCTCTTGAACCGGA
  TTCTGGTACCAGCTCTGCTGCTGATGGTCCTTGGACACAGAGAGGGGAGGAGGAGGAGGCAGAGGCCAGA
  GCCAAGCTGGCCCCAGGGAGGGAGCCCCCTAGTCCCTGCCACTCAGAGGACAGCCTTGGGCTGGGGGCAG
  CACCCCTTGGCAGCGAACCACCCCTGAGCCAGCTGGTGTCCGACTCAGACTCAGAGCTGGACAGCACAGA
  GCGGCTGGCCCTGGGAAGCACAGACACCTTGTCCAATGGGCAGAAAGCGGACCTGGAGGCTGCGCAGCGC
  CTGGCCAAGAGGCTGTACCGACTAGATGGCTTCAGGAAGGCCGATGTGGCCCGGCACCTGGGCAAGAACA
  ATGACTTCAGCAAACTGGTGGCTGGGGAGTACCTCAAGTTCTTTGTCTTCACGGGCATGACTCTGGACCA
  AGCTCTCAGGGTGTTTCTGAAGGAGCTGGCCTTAATGGGTGAGACCCAGGAACGAGAGCGCGTGCTGGCC
  CACTTCTCCCAGCGATACTTCCAGTGCAATCCTGAAGCCCTGTCCTCAGAGGACGGCGCCCACACGCTGA
  CCTGTGCGCTCATGCTGCTCAACACGGATCTCCACGGCCATAACATCGGGAAGCGCATGACCTGCGGGGA
  CTTCATCGGGAACCTGGAGGGCCTCAATGATGGCGGCGACTTCCCTAGGGAGCTGCTCAAGGCCTTGTAC
  AGCTCCATCAAGAATGAGAAGCTGCAGTGGGCCATAGACGAGGAGGAGCTGAGACGCTCTCTGTCTGAGT
  TGGCCGACCCCAACCCCAAGGTCATCAAGCGGATCAGCGGGGGCAGTGGCAGTGGCTCCAGCCCTTTCCT
  GGACCTGACTCCCGAGCCTGGGGCTGCCGTCTACAAGCACGGGGCCCTGGTGCGAAAGGTGCACGCAGAC
  CCTGACTGCAGGAAGACACCTCGGGGCAAGCGGGGCTGGAAGAGCTTCCACGGGATCCTCAAGGGCATGA
  TCCTCTACCTGCAGAAGGAGGAGTACAAGCCTGGGAAGGCCCTTTCAGAGACGGAGCTCAAGAATGCCAT
  CAGCATCCACCATGCCCTGGCCACTCGTGCCAGTGACTACAGCAAGAGGCCCCACGTCTTCTACCTGCGC
  ACAGCTGACTGGCGGGTCTTCCTCTTCCAGGCCCCGAGCCTGGAGCAGATGCAGTCCTGGATCACTCGCA
  TCAATGTAGTAGCCGCTATGTTCTCTGCGCCCCCCTTCCCAGCTGCTGTTAGCTCCCAAAAGAAGTTCAG
  CCGCCCTCTCCTGCCCAGCGCTGCCACCCGCCTCTCCCAGGAGGAGCAGGTGCGGACCCACGAGGCCAAG
  CTGAAGGCCATGGCAAGTGAGCTGCGGGAGCACCGGGCCGCCCAGCTGGGCAAGAAGGGCCGGGGCAAGG
  AGGCTGAAGAGCAGCGGCAGAAGGAGGCCTACCTGGAGTTTGAGAAATCCCGCTACAGCACCTATGCAGC
  GCTGCTTCGGGTCAAGCTGAAGGCAGGCAGTGAGGAGCTGGATGCAGTGGAGGCAGCACTGGCCCAGGCC
  GGGAGCACAGAGGATGGACTCCCTCCTTCTCACTCCAGTCCCTCCCTGCAGCCCAAACCCTCCAGCCAGC
  CCCGGGCTCAGCGTCACAGCTCAGAGCCTCGGCCAGGGGCAGGCAGTGGGCGGCGGAAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235126 representing NM_001270965
  Red=Cloning site Green=Tags(s)
  
  MAQGAMRFCSEGDCAISPPRCPRRWLPEGPVPQSPPASMYGSTGSLLRRVAGPGPRGRELGRVTAPCTPL
  RGPPSPRVAPSPWAPSSPTGQPPPGAQSSVVIFRFVEKASVRPLNGLPAPGGLSRSWDLGGVSPPRPTPA
  LGPGSNRKLRLEASTSDPLPARGGSALPGSRNLVHGPPAPPQVGADGLYSSLPNGLGGPPERLATLFGGP
  ADTGFLNQGDTWSSPREVSSHAQRIARAKWEFFYGSLDPPSSGAKPPEQAPPSPPGVGSRQGSGVAVGRA
  AKYSETDLDTVPLRCYRETDIDEVLAEREEADSAIESQPSSEGPPGTAYPPAPRPGPLPGPHPSLGSGNE
  DEDDDEAGGEEDVDDEVFEASEGARPGSRMPLKSPVPFLPGTSPSADGPDSFSCVFEAILESHRAKGTSY
  TSLASLEALASPGPTQSPFFTFELPPQPPAPRPDPPAPAPLAPLEPDSGTSSAADGPWTQRGEEEEAEAR
  AKLAPGREPPSPCHSEDSLGLGAAPLGSEPPLSQLVSDSDSELDSTERLALGSTDTLSNGQKADLEAAQR
  LAKRLYRLDGFRKADVARHLGKNNDFSKLVAGEYLKFFVFTGMTLDQALRVFLKELALMGETQERERVLA
  HFSQRYFQCNPEALSSEDGAHTLTCALMLLNTDLHGHNIGKRMTCGDFIGNLEGLNDGGDFPRELLKALY
  SSIKNEKLQWAIDEEELRRSLSELADPNPKVIKRISGGSGSGSSPFLDLTPEPGAAVYKHGALVRKVHAD
  PDCRKTPRGKRGWKSFHGILKGMILYLQKEEYKPGKALSETELKNAISIHHALATRASDYSKRPHVFYLR
  TADWRVFLFQAPSLEQMQSWITRINVVAAMFSAPPFPAAVSSQKKFSRPLLPSAATRLSQEEQVRTHEAK
  LKAMASELREHRAAQLGKKGRGKEAEEQRQKEAYLEFEKSRYSTYAALLRVKLKAGSEELDAVEAALAQA
  GSTEDGLPPSHSSPSLQPKPSSQPRAQRHSSEPRPGAGSGRRKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270965
• ORF Size: 3072 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270965.1, NP_001257894.1
• RefSeq Size: 3861 bp
• RefSeq ORF: 3075 bp
• Locus ID: 5662
• Cytogenetics: 10q24.32
• Protein Pathways: Endocytosis
• MW: 109.5 kDa
• Gene Summary: 'This gene encodes a Plekstrin homology and SEC7 domains-containing protein that functions as a guanine nucleotide exchange factor. The encoded protein regulates signal transduction by activating ADP-ribosylation factor 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]'