CUX1 (NM_001202543) Human Tagged ORF Clone

CAT#: RC235316

CUX1 (Myc-DDK tagged) - Homo sapiens cut-like homeobox 1 (CUX1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CUX1
• Synonyms: CASP; CDP; CDP/Cut; CDP1; Clox; COY1; CUTL1; CUX; Cux/CDP; GDDI; GOLIM6; Nbla10317; p75; p100; p110; p200
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235316 representing NM_001202543
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCAATGTGGGATCGATGTTTCAATATTGGAAGCGCTTTGATTTACAGCAGCTGCAGAGAGAAC
  TCGATGCCACCGCAACGGTATTGGCGAACCGGCAGGATGAAAGTGAGCAGTCCAGAAAGCGGCTTATCGA
  ACAGAGCCGGGAGTTCAAGAAGAACACTCCAGAGGATTTGCGCAAGCAGGTAGCGCCGCTGCTGAAGAGT
  TTCCAAGGAGAGATTGATGCACTGAGTAAAAGAAGCAAGGAAGCTGAAGCAGCTTTCTTGAATGTCTACA
  AAAGATTGATTGACGTCCCAGATCCCGTACCAGCTTTGGATCTCGGACAGCAACTCCAGCTCAAAGTGCA
  GCGCCTGCACGATATTGAAACAGAGAACCAGAAACTTAGGGAAACTCTGGAAGAATACAACAAGGAATTT
  GCTGAAGTGAAAAATCAAGAGGTTACGATAAAAGCACTTAAAGAGAAAATCCGAGAATATGAACAGACAC
  TGAAGAACCAAGCCGAAACCATAGCTCTTGAGAAGGAACAGAAGTTACAGAATGACTTTGCAGAAAAGGA
  GAGAAAGCTGCAGGAGACACAGATGTCCACCACCTCAAAGCTGGAGGAAGCTGAGCATAAGGTTCAGAGC
  CTACAAACAGCCCTGGAAAAAACTCGAACAGAATTATTTGACCTGAAAACCAAATACGATGAAGAAACTA
  CTGCAAAGGCCGACGAGATTGAAATGATCATGACGGACCTTGAAAGGGCAAACCAGAGGGCAGAGGTGGC
  TCAGAGAGAGGCGGAGACCTTAAGGGAACAGCTCTCATCGGCCAATCACTCCCTCCAGCTGGCCTCACAG
  ATCCAGAAGGCACCAGACGTGGAGCAGGCCATAGAGGTGCTGACCCGCTCCAGCCTAGAAGTTGAGTTGG
  CCGCCAAGGAGCGGGAGATCGCACAGCTGGTGGAGGACGTGCAGAGACTCCAGGCCAGCCTCACCAAGCT
  GCGGGAGAATTCGGCCAGCCAGATCTCACAGCTTGAGCAGCAGCTGAGCGCCAAAAACAGCACACTCAAA
  CAACTGGAAGAAAAACTCAAAGGCCAGGCTGACTATGAAGAGGTGAAGAAAGAGCTGAACATTCTGAAGT
  CCATGGAGTTTGCACCGTCCGAGGGCGCTGGGACACAGGATGCGGCCAAGCCCCTGGAGGTGCTGTTGCT
  GGAGAAGAACCGCTCGCTGCAGTCCGAGAACGCCGCGCTGCGCATCTCCAACAGCGACCTGAGCGGGTCA
  GCCAGGAGGAAAGGGAAAGACCAGCCTGAAAGTCGGCGCCCGGGATCTTTGCCGGCCCCCCCTCCTTCTC
  AGTTGCCCCGCAACCCGGGGGAGCAGGCTTCCAATACTAATGGTACACACCAGTTCTCACCAGCGGGGTT
  AAGTCAAGACTTTTTCAGCTCATCCCTGGCAAGCCCCAGCCTACCCCTGGCTTCTACAGGAAAATTTGCA
  CTAAACTCTCTTCTCCAGCGGCAGCTAATGCAGTCCTTCTACTCCAAGGCTATGCAGGAAGCCGGAAGCA
  CAAGCATGATTTTTTCAACAGGTCCATACAGCACAAACTCCATATCTTCCCAAAGTCCATTACAACAAAG
  CCCAGATGTCAATGGCATGGCCCCATCCCCCAGCCAGTCAGAAAGTGCTGGGAGCGTCTCCGAGGGCGAG
  GAGATGGACACTGCAGAAATCGCCCGGCAGGTCAAAGAGCAGCTGATTAAGCACAATATCGGACAACGTA
  TTTTCGGACATTATGTGTTGGGACTGTCTCAAGGGTCCGTGAGCGAGATTCTGGCCCGGCCCAAGCCATG
  GAATAAACTGACTGTTCGTGGCAAGGAGCCATTTCACAAGATGAAACAGTTCCTCTCCGATGAGCAGAAC
  ATCCTGGCCCTCCGTAGCATCCAAGGCAGACAAAGAGAGAATCCAGGCCAGAGCCTGAACAGACTATTTC
  AGGAAGTACCGAAACGAAGAAATGGGTCTGAAGGTAACATCACCACCCGGATCCGAGCCTCGGAGACTGG
  CTCTGATGAAGCCATCAAGTCCATCCTAGAGCAAGCCAAGAGGGAGCTCCAAGTGCAGAAAACTGCAGAG
  CCGGCCCAGCCTTCCTCCGCATCCGGCAGCGGGAACTCTGATGACGCCATCCGCTCCATCCTGCAGCAAG
  CCCGCCGGGAGATGGAGGCCCAGCAGGCTGCCCTCGACCCTGCCTTAAAGCAGGCACCACTGTCCCAGAG
  TGACATCACCATCCTCACCCCCAAGCTTCTGTCCACCTCGCCCATGCCCACCGTGTCCAGCTACCCACCT
  CTCGCCATCTCCCTGAAGAAGCCCTCCGCAGCTCCTGAGGCCGGTGCCTCTGCTCTGCCGAACCCCCCGG
  CCCTCAAAAAGGAGGCCCAGGACGCCCCCGGGCTGGACCCCCAGGGAGCAGCCGATTGTGCACAAGGGGT
  CCTGAGACAGGTGAAAAATGAGGTGGGCCGCAGCGGTGCCTGGAAGGACCACTGGTGGAGCGCGGTGCAG
  CCGGAGAGAAGAAATGCCGCCTCCTCCGAGGAGGCCAAGGCCGAAGAAACGGGCGGCGGGAAAGAGAAGG
  GCAGCGGTGGCAGCGGAGGTGGCAGCCAGCCTCGGGCCGAGCGCAGTCAGCTCCAGGGACCCTCGTCGTC
  AGAGTACTGGAAGGAGTGGCCCAGCGCTGAGTCCCCATACTCCCAGAGCTCAGAGCTGAGTCTGACCGGG
  GCCAGCCGCAGCGAGACACCACAGAACAGCCCCCTGCCATCCTCCCCGATCGTGCCCATGTCCAAGCCCA
  CCAAGCCCTCGGTCCCCCCGCTGACCCCCGAGCAGTACGAGGTCTACATGTACCAGGAGGTGGACACCAT
  CGAGCTCACCCGGCAGGTTAAGGAAAAGCTGGCCAAGAACGGCATCTGCCAGAGAATCTTCGGGGAGAAG
  GTGCTGGGCCTGTCCCAGGGCAGCGTCAGCGACATGCTGTCCCGACCGAAGCCATGGAGCAAGCTGACGC
  AGAAAGGCCGAGAACCCTTCATCCGGATGCAGCTCTGGCTGAACGGCGAGCTAGGCCAGGGTGTTCTACC
  CGTCCAGGGCCAGCAGCAAGGGCCAGTCCTCCACTCCGTGACATCGCTCCAGGACCCGCTGCAGCAGGGC
  TGTGTGAGCTCAGAAAGCACTCCAAAGACCTCCGCCAGCTGCAGCCCTGCCCCTGAGTCCCCGATGAGTT
  CCAGTGAGTCGGTGAAGAGCCTGACCGAGCTGGTCCAGCAGCCCTGTCCCCCCATCGAGGCGAGCAAGGA
  CAGCAAGCCACCAGAGCCCAGTGACCCGCCAGCATCCGACTCCCAGCCCACAACCCCGCTGCCTCTCTCC
  GGACACTCGGCCCTCAGCATCCAAGAATTAGTAGCCATGTCCCCGGAGCTGGACACCTACGGCATAACCA
  AGCGGGTGAAGGAGGTGCTGACGGACAACAACCTCGGCCAGCGCTTATTTGGGGAGACCATCTTAGGGCT
  CACCCAAGGCTCTGTCTCTGACCTCCTTGCCCGCCCCAAACCCTGGCATAAGCTCAGTCTGAAAGGACGA
  GAGCCCTTCGTCCGGATGCAGCTGTGGCTGAACGACCCCAACAATGTGGAGAAGCTGATGGACATGAAAC
  GGATGGAGAAGAAAGCCTACATGAAGCGGCGGCACAGCTCAGTCAGTGACAGCCAGCCCTGCGAACCGCC
  CTCTGTCGGCACCGAGTACAGCCAGGGCGCCAGCCCCCAGCCCCAGCACCAGCTGAAGAAACCCCGGGTG
  GTGCTGGCTCCGGAGGAGAAGGAGGCGCTGAAACGAGCGTATCAGCAAAAGCCATACCCGTCACCAAAAA
  CCATCGAAGACCTCGCCACCCAGCTCAACCTGAAAACCAGCACCGTCATCAACTGGTTCCACAACTACAG
  GTCTCGGATCCGCAGAGAACTGTTCATTGAGGAAATTCAGGCCGGGAGTCAGGGCCAGGCGGGCGCCAGC
  GACTCACCCTCGGCCCGCAGCGGCCGGGCGGCGCCCAGCTCGGAGGGCGACAGCTGCGACGGCGTGGAGG
  CCACTGAGGGCCCAGGCAGCGCCGACACCGAGGAGCCCAAGTCTCAGGGAGAGGCCGAGCGGGAGGAGGT
  GCCGCGGCCGGCGGAGCAGACGGAGCCGCCGCCCTCGGGGACCCCGGGCCCGGACGACGCCCGCGACGAC
  GACCACGAGGGAGGCCCCGTGGAAGGCCCGGGGCCCCTGCCCAGCCCCGCCTCCGCGACCGCCACCGCCG
  CGCCCGCGGCCCCCGAGGACGCCGCTACCTCAGCCGCCGCCGCGCCGGGGGAGGGCCCCGCGGCCCCGAG
  CTCCGCGCCGCCGCCCAGCAACAGCAGCAGCAGCAGCGCCCCCCGCAGGCCCAGCTCGCTGCAGAGCCTT
  TTCGGCCTCCCCGAGGCCGCGGGCGCCCGGGACTCGCGCGACAACCCCCTGCGCAAGAAGAAGGCCGCGA
  ACTTGAACAGCATCATCCACCGCCTGGAGAAGGCCGCCAGCCGGGAGGAACCTATCGAATGGGAGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235316 representing NM_001202543
  Red=Cloning site Green=Tags(s)
  
  MAANVGSMFQYWKRFDLQQLQRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKS
  FQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRETLEEYNKEF
  AEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQS
  LQTALEKTRTELFDLKTKYDEETTAKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQ
  IQKAPDVEQAIEVLTRSSLEVELAAKEREIAQLVEDVQRLQASLTKLRENSASQISQLEQQLSAKNSTLK
  QLEEKLKGQADYEEVKKELNILKSMEFAPSEGAGTQDAAKPLEVLLLEKNRSLQSENAALRISNSDLSGS
  ARRKGKDQPESRRPGSLPAPPPSQLPRNPGEQASNTNGTHQFSPAGLSQDFFSSSLASPSLPLASTGKFA
  LNSLLQRQLMQSFYSKAMQEAGSTSMIFSTGPYSTNSISSQSPLQQSPDVNGMAPSPSQSESAGSVSEGE
  EMDTAEIARQVKEQLIKHNIGQRIFGHYVLGLSQGSVSEILARPKPWNKLTVRGKEPFHKMKQFLSDEQN
  ILALRSIQGRQRENPGQSLNRLFQEVPKRRNGSEGNITTRIRASETGSDEAIKSILEQAKRELQVQKTAE
  PAQPSSASGSGNSDDAIRSILQQARREMEAQQAALDPALKQAPLSQSDITILTPKLLSTSPMPTVSSYPP
  LAISLKKPSAAPEAGASALPNPPALKKEAQDAPGLDPQGAADCAQGVLRQVKNEVGRSGAWKDHWWSAVQ
  PERRNAASSEEAKAEETGGGKEKGSGGSGGGSQPRAERSQLQGPSSSEYWKEWPSAESPYSQSSELSLTG
  ASRSETPQNSPLPSSPIVPMSKPTKPSVPPLTPEQYEVYMYQEVDTIELTRQVKEKLAKNGICQRIFGEK
  VLGLSQGSVSDMLSRPKPWSKLTQKGREPFIRMQLWLNGELGQGVLPVQGQQQGPVLHSVTSLQDPLQQG
  CVSSESTPKTSASCSPAPESPMSSSESVKSLTELVQQPCPPIEASKDSKPPEPSDPPASDSQPTTPLPLS
  GHSALSIQELVAMSPELDTYGITKRVKEVLTDNNLGQRLFGETILGLTQGSVSDLLARPKPWHKLSLKGR
  EPFVRMQLWLNDPNNVEKLMDMKRMEKKAYMKRRHSSVSDSQPCEPPSVGTEYSQGASPQPQHQLKKPRV
  VLAPEEKEALKRAYQQKPYPSPKTIEDLATQLNLKTSTVINWFHNYRSRIRRELFIEEIQAGSQGQAGAS
  DSPSARSGRAAPSSEGDSCDGVEATEGPGSADTEEPKSQGEAEREEVPRPAEQTEPPPSGTPGPDDARDD
  DHEGGPVEGPGPLPSPASATATAAPAAPEDAATSAAAAPGEGPAAPSSAPPPSNSSSSSAPRRPSSLQSL
  FGLPEAAGARDSRDNPLRKKKAANLNSIIHRLEKAASREEPIEWEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001202543
• ORF Size: 4548 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001202543.1, NP_001189472.1
• RefSeq Size: 13882 bp
• RefSeq ORF: 4551 bp
• Locus ID: 1523
• Cytogenetics: 7q22.1
• Protein Families: Transcription Factors, Transmembrane
• MW: 165.7 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Feb 2011]'