TRPM1 (NM_001252020) Human Tagged ORF Clone

CAT#: RC235326

TRPM1 (Myc-DDK tagged) - Homo sapiens transient receptor potential cation channel, subfamily M, member 1 (TRPM1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRPM1
• Synonyms: CSNB1C; LTRPC1; MLSN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235326 representing NM_001252020
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCTTCAAGCGGGGCTCGCTCAAGAGCTCCACATCAGGGTCGCAGAAGGGTCAGAAATCTTGGA
  TAGAGAAAACCTTTTGCAAACGGGAATGTATCTTTGTAATTCCTAGCATGAAAGACTCTAACAGGTGTTG
  CTGTGGCCAGTTCACCAACCAGCATATCCCCCCTCTGCCAAGTGCAACACCCAGCAAAAATGAAGAGGAA
  AGCAAACAGGTGGAGACTCAGCCTGAGAAATGGTCTGTTGCCAAGCACACCCAGAGCTACCCAACAGATT
  CCTATGGAGTTCTTGAATTCCAGGGTGGCGGATATTCCAATAAAGCCATGTATATCCGTGTATCCTATGA
  CACCAAGCCAGACTCACTGCTCCATCTCATGGTGAAAGATTGGCAGCTGGAACTCCCCAAGCTCTTAATA
  TCTGTGCATGGAGGCCTCCAGAACTTTGAGATGCAGCCCAAGCTGAAACAAGTCTTTGGGAAAGGCCTGA
  TCAAGGCTGCTATGACCACCGGGGCCTGGATCTTCACCGGGGGTGTCAGCACAGGTGTTATCAGCCACGT
  AGGGGATGCCTTGAAAGACCACTCCTCCAAGTCCAGAGGCCGGGTTTGTGCTATAGGAATTGCTCCATGG
  GGCATCGTGGAGAATAAGGAAGACCTGGTTGGAAAGGATGTAACAAGAGTGTACCAGACCATGTCCAACC
  CTCTAAGTAAGCTCTCTGTGCTCAACAACTCCCACACCCACTTCATCCTGGCTGACAATGGCACCCTGGG
  CAAGTATGGCGCCGAGGTGAAGCTGCGAAGGCTGCTGGAAAAGCACATCTCCCTGCAGAAGATCAACACA
  AGACTGGGGCAGGGCGTGCCCCTCGTGGGTCTCGTGGTGGAGGGGGGCCCTAACGTGGTGTCCATCGTCT
  TGGAATACCTGCAAGAAGAGCCTCCCATCCCTGTGGTGATTTGTGATGGCAGCGGACGTGCCTCGGACAT
  CCTGTCCTTTGCGCACAAGTACTGTGAAGAAGGCGGAATAATAAATGAGTCCCTCAGGGAGCAGCTTCTA
  GTTACCATTCAGAAAACATTTAATTATAATAAGGCACAATCACATCAGCTGTTTGCAATTATAATGGAGT
  GCATGAAGAAGAAAGAACTCGTCACTGTGTTCAGAATGGGTTCTGAGGGCCAGCAGGACATCGAGATGGC
  AATTTTAACTGCCCTGCTGAAAGGAACAAACGTATCTGCTCCAGATCAGCTGAGCTTGGCACTGGCTTGG
  AACCGCGTGGACATAGCACGAAGCCAGATCTTTGTCTTTGGGCCCCACTGGCCGCCCCTGGGAAGCCTGG
  CACCCCCGACGGACAGCAAAGCCACGGAGAAGGAGAAGAAGCCACCCATGGCCACCACCAAGGGAGGAAG
  AGGAAAAGGGAAAGGCAAGAAGAAAGGGAAAGTGAAAGAGGAAGTGGAGGAAGAAACTGACCCCCGGAAG
  ATAGAGCTGCTGAACTGGGTGAATGCTTTGGAGCAAGCGATGCTAGATGCTTTAGTCTTAGATCGTGTCG
  ACTTTGTGAAGCTCCTGATTGAAAACGGAGTGAACATGCAACACTTTCTGACCATTCCGAGGCTGGAGGA
  GCTTTATAACACAAGACTGGGTCCACCAAACACACTTCATCTGCTGGTGAGGGATGTGAAAAAGAGCAAC
  CTTCCGCCTGATTACCACATCAGCCTCATAGACATCGGGCTCGTGCTGGAGTACCTCATGGGAGGAGCCT
  ACCGCTGCAACTACACTCGGAAAAACTTTCGGACCCTTTACAACAACTTGTTTGGACCAAAGAGGCCTAA
  AGCTCTTAAACTTCTGGGAATGGAAGATGATGAGCCTCCAGCTAAAGGGAAGAAAAAGAAAAAGAAGAAA
  AAGGAGGAAGAGATCGACATTGATGTGGACGACCCTGCCGTGAGTCGGTTCCAGTATCCCTTCCACGAGC
  TGATGGTGTGGGCAGTGCTGATGAAACGCCAGAAAATGGCAGTGTTCCTCTGGCAGCGAGGGGAAGAGAG
  CATGGCCAAGGCCCTGGTGGCCTGCAAGCTCTACAAGGCCATGGCCCACGAGTCCTCCGAGAGTGATCTG
  GTGGATGACATCTCCCAGGACTTGGATAACAATTCCAAAGACTTCGGCCAGCTTGCTTTGGAGTTATTAG
  ACCAGTCCTATAAGCATGACGAGCAGATCGCTATGAAACTCCTGACCTACGAGCTGAAAAACTGGAGCAA
  CTCGACCTGCCTCAAACTGGCCGTGGCAGCCAAACACCGGGACTTCATTGCTCACACCTGCAGCCAGATG
  CTGCTGACCGATATGTGGATGGGAAGACTGCGGATGCGGAAGAACCCCGGCCTGAAGGTTATCATGGGGA
  TTCTTCTACCCCCCACCATCTTGTTTTTGGAATTTCGCACATATGATGATTTCTCGTATCAAACATCCAA
  GGAAAATGAGGATGGCAAAGAAAAAGAAGAGGAAAATACGGATGCAAATGCAGATGCTGGCTCAAGAAAG
  GGGGATGAGGAGAACGAGCACAAAAAACAGAGAAGTATTCCCATCGGAACAAAGATCTGTGAATTCTATA
  ACGCGCCCATTGTCAAGTTCTGGTTTTACACAATATCATACTTGGGCTACCTGCTGCTGTTTAACTACGT
  CATCCTGGTGCGGATGGATGGCTGGCCGTCCCTCCAGGAGTGGATCGTCATCTCCTACATCGTGAGCCTG
  GCGTTAGAGAAGATACGAGAGATCCTCATGTCAGAACCAGGCAAACTCAGCCAGAAAATCAAAGTTTGGC
  TTCAGGAGTACTGGAACATCACAGATCTCGTGGCCATTTCCACATTCATGATTGGAGCAATTCTTCGCCT
  ACAGAACCAGCCCTACATGGGCTATGGCCGGGTGATCTACTGTGTGGATATCATCTTCTGGTACATCCGT
  GTCCTGGACATCTTTGGTGTCAACAAGTATCTGGGGCCATACGTGATGATGATTGGAAAGATGATGATCG
  ACATGCTGTACTTTGTGGTCATCATGCTGGTCGTGCTCATGAGTTTCGGAGTAGCCCGTCAAGCCATTCT
  GCATCCAGAGGAGAAGCCCTCTTGGAAACTGGCCCGAAACATCTTCTACATGCCCTACTGGATGATCTAT
  GGAGAGGTGTTTGCAGACCAGATAGACCTCTACGCCATGGAAATTAATCCTCCTTGTGGTGAGAACCTAT
  ATGATGAGGAGGGCAAGCGGCTTCCTCCCTGTATCCCCGGCGCCTGGCTCACTCCAGCACTCATGGCGTG
  CTATCTACTGGTCGCCAACATCCTGCTGGTGAACCTGCTGATTGCTGTGTTCAACAATACCTTCTTTGAA
  GTAAAATCAATATCCAACCAGGTGTGGAAGTTCCAGCGATATCAGCTGATTATGACATTTCATGACAGGC
  CAGTCCTGCCCCCACCGATGATCATTTTAAGCCACATCTACATCATCATTATGCGTCTCAGCGGCCGCTG
  CAGGAAAAAGAGAGAAGGGGACCAAGAGGAACGGGATCGTGGATTGAAGCTCTTCCTTAGCGACGAGGAG
  CTAAAGAGGCTGCATGAGTTCGAGGAGCAGTGCGTGCAGGAGCACTTCCGGGAGAAGGAGGATGAGCAGC
  AGTCGTCCAGCGACGAGCGCATCCGGGTCACTTCTGAAAGAGTTGAAAATATGTCAATGAGGTTGGAAGA
  AATCAATGAAAGAGAAACTTTTATGAAAACTTCCCTGCAGACTGTTGACCTTCGACTTGCTCAGCTAGAA
  GAATTATCTAACAGAATGGTGAATGCTCTTGAAAATCTTGCGGGAATCGACAGGTCTGACCTGATCCAGG
  CACGGTCCCGGGCTTCTTCTGAATGTGAGGCAACGTATCTTCTCCGGCAAAGCAGCATCAATAGCGCTGA
  TGGCTACAGCTTGTATCGATATCATTTTAACGGAGAAGAGTTATTATTTGAGGATACATCTCTCTCCACG
  TCACCAGGGACAGGAGTCAGGAAAAAAACCTGTTCCTTCCGTATAAAGGAAGAGAAGGACGTGAAAACGC
  ACCTAGTCCCAGAATGTCAGAACAGTCTTCACCTTTCACTGGGCACAAGCACATCAGCAACCCCAGATGG
  CAGTCACCTTGCAGTAGATGACTTAAAGAACGCTGAAGAGTCAAAATTAGGTCCAGATATTGGGATTTCA
  AAGGAAGATGATGAAAGACAGACAGACTCTAAAAAAGAAGAAACTATTTCCCCAAGTTTAAATAAAACAG
  ATGTGATACATGGACAGGACAAATCAGATGTTCAAAACACTCAGCTAACAGTGGAAACGACAAATATAGA
  AGGCACTATTTCCTATCCCCTGGAAGAAACCAAAATTACACGCTATTTCCCCGATGAAACGATCAATGCT
  TGTAAAACAATGAAGTCCAGAAGCTTCGTCTATTCCCGGGGAAGAAAGCTGGTCGGTGGGGTTAACCAGG
  ATGTAGAGTACAGTTCAATCACGGACCAGCAATTGACGACGGAATGGCAATGCCAAGTTCAAAAGATCAC
  GCGCTCTCATAGCACAGATATTCCTTACATTGTGTCGGAAGCTGCAGTGCAAGCTGAGCATAAAGAGCAG
  TTTGCAGATATGCAAGATGAACACCATGTCGCTGAAGCAATTCCTCGAATCCCTCGCTTGTCCCTAACCA
  TTACTGACAGAAATGGGATGGAAAACTTACTGTCTGTGAAGCCAGATCAAACTTTGGGATTCCCATCTCT
  CAGGTCAAAAAGTTTACATGGACATCCTAGGAATGTGAAATCCATTCAGGGAAAGTTAGACAGATCTGGA
  CATGCCAGTAGTGTAAGCAGCTTAGTAATTGTGTCTGGAATGACAGCAGAAGAAAAAAAGGTTAAGAAAG
  AGAAAGCTTCCACAGAAACTGAATGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235326 representing NM_001252020
  Red=Cloning site Green=Tags(s)
  
  MSSFKRGSLKSSTSGSQKGQKSWIEKTFCKRECIFVIPSMKDSNRCCCGQFTNQHIPPLPSATPSKNEEE
  SKQVETQPEKWSVAKHTQSYPTDSYGVLEFQGGGYSNKAMYIRVSYDTKPDSLLHLMVKDWQLELPKLLI
  SVHGGLQNFEMQPKLKQVFGKGLIKAAMTTGAWIFTGGVSTGVISHVGDALKDHSSKSRGRVCAIGIAPW
  GIVENKEDLVGKDVTRVYQTMSNPLSKLSVLNNSHTHFILADNGTLGKYGAEVKLRRLLEKHISLQKINT
  RLGQGVPLVGLVVEGGPNVVSIVLEYLQEEPPIPVVICDGSGRASDILSFAHKYCEEGGIINESLREQLL
  VTIQKTFNYNKAQSHQLFAIIMECMKKKELVTVFRMGSEGQQDIEMAILTALLKGTNVSAPDQLSLALAW
  NRVDIARSQIFVFGPHWPPLGSLAPPTDSKATEKEKKPPMATTKGGRGKGKGKKKGKVKEEVEEETDPRK
  IELLNWVNALEQAMLDALVLDRVDFVKLLIENGVNMQHFLTIPRLEELYNTRLGPPNTLHLLVRDVKKSN
  LPPDYHISLIDIGLVLEYLMGGAYRCNYTRKNFRTLYNNLFGPKRPKALKLLGMEDDEPPAKGKKKKKKK
  KEEEIDIDVDDPAVSRFQYPFHELMVWAVLMKRQKMAVFLWQRGEESMAKALVACKLYKAMAHESSESDL
  VDDISQDLDNNSKDFGQLALELLDQSYKHDEQIAMKLLTYELKNWSNSTCLKLAVAAKHRDFIAHTCSQM
  LLTDMWMGRLRMRKNPGLKVIMGILLPPTILFLEFRTYDDFSYQTSKENEDGKEKEEENTDANADAGSRK
  GDEENEHKKQRSIPIGTKICEFYNAPIVKFWFYTISYLGYLLLFNYVILVRMDGWPSLQEWIVISYIVSL
  ALEKIREILMSEPGKLSQKIKVWLQEYWNITDLVAISTFMIGAILRLQNQPYMGYGRVIYCVDIIFWYIR
  VLDIFGVNKYLGPYVMMIGKMMIDMLYFVVIMLVVLMSFGVARQAILHPEEKPSWKLARNIFYMPYWMIY
  GEVFADQIDLYAMEINPPCGENLYDEEGKRLPPCIPGAWLTPALMACYLLVANILLVNLLIAVFNNTFFE
  VKSISNQVWKFQRYQLIMTFHDRPVLPPPMIILSHIYIIIMRLSGRCRKKREGDQEERDRGLKLFLSDEE
  LKRLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVENMSMRLEEINERETFMKTSLQTVDLRLAQLE
  ELSNRMVNALENLAGIDRSDLIQARSRASSECEATYLLRQSSINSADGYSLYRYHFNGEELLFEDTSLST
  SPGTGVRKKTCSFRIKEEKDVKTHLVPECQNSLHLSLGTSTSATPDGSHLAVDDLKNAEESKLGPDIGIS
  KEDDERQTDSKKEETISPSLNKTDVIHGQDKSDVQNTQLTVETTNIEGTISYPLEETKITRYFPDETINA
  CKTMKSRSFVYSRGRKLVGGVNQDVEYSSITDQQLTTEWQCQVQKITRSHSTDIPYIVSEAAVQAEHKEQ
  FADMQDEHHVAEAIPRIPRLSLTITDRNGMENLLSVKPDQTLGFPSLRSKSLHGHPRNVKSIQGKLDRSG
  HASSVSSLVIVSGMTAEEKKVKKEKASTETEC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252020
• ORF Size: 4926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252020.1, NP_001238949.1
• RefSeq Size: 6022 bp
• RefSeq ORF: 4929 bp
• Locus ID: 4308
• Cytogenetics: 15q13.3
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• MW: 187 kDa
• Gene Summary: 'This gene encodes a member of the transient receptor potential melastatin subfamily of transient receptor potential ion channels. The encoded protein is a calcium permeable cation channel that is expressed in melanocytes and may play a role in melanin synthesis. Specific mutations in this gene are the cause autosomal recessive complete congenital stationary night blindness-1C. The expression of this protein is inversely correlated with melanoma aggressiveness and as such it is used as a prognostic marker for melanoma metastasis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011]'