SLC35A2 (NM_001282648) Human Tagged ORF Clone

CAT#: RC236658

SLC35A2 (myc-DDK-tagged) - Human solute carrier family 35 (UDP-galactose transporter), member A2 (SLC35A2), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC35A2
• Synonyms: CDG2M; CDGX; UDP-Gal-Tr; UGALT; UGAT; UGT; UGT1; UGT2; UGTL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC236658 representing NM_001282648
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTCTGTAGGGATGCTCACAGGCGCCTGAAGTACATATCCCTAGCTGTGCTGGTGGTCCAGAATG
  CCTCCCTCATCCTCAGCATCCGCTACGCCCGCACGTTGCCAGGGGACCGCTTCTTTGCCACCACTGCTGT
  GGTCATGGCGGAAGTGCTCAAAGGTCTCACCTGCCTGCTGCTGCTCTTCGCACAGAAGAGGGGTAACGTG
  AAGCACCTGGTTCTCTTCCTCCATGAGGCTGTCCTGGTGCAGTATGTGGACACGCTCAAGCTCGCAGTGC
  CCTCTCTCATCTACACCTTGCAGAATAACCTCCAGTATGTTGCCATCTCTAACCTACCAGCTGCCACTTT
  CCAGCCTTCCCCGAGGTGCAGCCAAAGCCATAGCCTCTGCCTCTGCCTCCGCCTCCGGGCCCTGCGTTCA
  CCAGCAGCCTCCCGGGCAGCCACCACCACCGCAGCTGTCTTCCCACCGTGGAGACCTCATCACGGAGCCC
  TTTCTGCCAAAGTCAGTGCTGGTGAAGTGAGGGCTGGCAGCAATGGGGGGACACAAGGGAGGGGGACTGG
  GGTGGAGGGTGTTGGGCATCTGCAGGACCCAAGTCGCCACCCTCCGGGGCCTGGCTCCTCTGGGTTTGGG
  AGATGGTCTTTTCTCCCAGGTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC236658 representing NM_001282648
  Red=Cloning site Green=Tags(s)
  
  MKLCRDAHRRLKYISLAVLVVQNASLILSIRYARTLPGDRFFATTAVVMAEVLKGLTCLLLLFAQKRGNV
  KHLVLFLHEAVLVQYVDTLKLAVPSLIYTLQNNLQYVAISNLPAATFQPSPRCSQSHSLCLCLRLRALRS
  PAASRAATTTAAVFPPWRPHHGALSAKVSAGEVRAGSNGGTQGRGTGVEGVGHLQDPSRHPPGPGSSGFG
  RWSFLPGH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282648
• ORF Size: 654 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282648.1, NP_001269577.1
• RefSeq Size: 1716 bp
• RefSeq ORF: 657 bp
• Locus ID: 7355
• Cytogenetics: Xp11.23
• Protein Families: Transmembrane
• MW: 23.9 kDa
• Gene Summary: 'This gene encodes a member of the nucleotide-sugar transporter family. The encoded protein is a multi-pass membrane protein. It transports UDP-galactose from the cytosol into Golgi vesicles, where it serves as a glycosyl donor for the generation of glycans. Mutations in this gene cause congenital disorder of glycosylation type IIm (CDG2M). Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2014]'