BPNT1 (NM_001286150) Human Tagged ORF Clone

CAT#: RC237076

BPNT1 (myc-DDK-tagged) - Human 3'(2'), 5'-bisphosphate nucleotidase 1 (BPNT1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BPNT1
• Synonyms: HEL20; PIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237076 representing NM_001286150
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCAGTAACACTGTGTTGATGCGGTTGGTAGCCTCCGCATATTCTATTGCTCAAAAGGCAGGAA
  TGATAGTCAGACGTGTTATTGCTGAAGGAGACCTGGGTATTGTGGAGAAGACCTGTGCAACAGACCTGCA
  GACCAAAGCTGACCGATTGGCACAGATGAGCATATGTTCTTCATTGGCCCGGAAATTCCCCAAACTCACA
  ATTATAGGGGAAGAGCTCGTGGTCTGGGTTGATCCTCTGGATGGAACCAAGGAATATACCGAAGGTCTTC
  TTGACAATGTAACAGTTCTTATTGGAATTGCTTATGAAGGAAAAGCCATAGCAGGAGTTATTAACCAGCC
  ATATTACAACTATGAGGCAGGACCAGATGCTGTGTTGGGGAGGACAATCTGGGGAGTTTTAGGTTTAGGC
  GCCTTTGGGTTTCAGCTGAAAGAAGTCCCTGCTGGGAAACACATTATCACAACTACTCGATCCCATAGCA
  ACAAGTTGGTTACTGACTGTGTTGCTGCTATGAACCCCGATGCTGTGCTGCGAGTAGGAGGAGCAGGAAA
  TAAGATTATTCAGCTGATTGAAGGCAAAGCCTCTGCTTATGTATTTGCAAGTCCTGGTTGTAAGAAGTGG
  GATACTTGTGCTCCAGAAGTTATTTTACATGCTGTGGGAGGCAAGTTAACCGATATCCATGGGAATGTTC
  TTCAGTACCACAAGGATGTGAAGCATATGAACTCTGCAGGAGTCCTGGCCACACTGAGGAATTATGACTA
  CTATGCAAGCCGAGTTCCAGAATCTATTAAAAATGCACTTGTTCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237076 representing NM_001286150
  Red=Cloning site Green=Tags(s)
  
  MASSNTVLMRLVASAYSIAQKAGMIVRRVIAEGDLGIVEKTCATDLQTKADRLAQMSICSSLARKFPKLT
  IIGEELVVWVDPLDGTKEYTEGLLDNVTVLIGIAYEGKAIAGVINQPYYNYEAGPDAVLGRTIWGVLGLG
  AFGFQLKEVPAGKHIITTTRSHSNKLVTDCVAAMNPDAVLRVGGAGNKIIQLIEGKASAYVFASPGCKKW
  DTCAPEVILHAVGGKLTDIHGNVLQYHKDVKHMNSAGVLATLRNYDYYASRVPESIKNALVP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286150
• ORF Size: 816 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001286150.1, NP_001273079.1
• RefSeq Size: 2357
• RefSeq ORF: 819
• Locus ID: 10380
• Protein Pathways: Sulfur metabolism
• MW: 29.6 kDa
• Gene Summary: BPNT1, also called bisphosphate 3-prime-nucleotidase, or BPntase, is a member of a magnesium-dependent phosphomonoesterase family. Lithium, a major drug used to treat manic depression, acts as an uncompetitive inhibitor of BPntase. The predicted human protein is 92% identical to mouse BPntase. BPntase's physiologic role in nucleotide metabolism may be regulated by inositol signaling pathways. The inhibition of human BPntase may account for lithium-induced nephrotoxicity. [provided by RefSeq, Jul 2008]