RPS10 (NM_001202470) Human Tagged ORF Clone

CAT#: RC237231

RPS10 (myc-DDK-tagged) - Human RPS10-NUDT3 readthrough (RPS10-NUDT3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPS10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237231 representing NM_001202470
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGATGCCTAAGAAGAACCGGATTGCCATTTATGAACTCCTTTTTAAGGAGGGAGTCATGGTGGCCA
  AGAAGGATGTCCACATGCCTAAGCACCCGGAGCTGGCAGACAAGAATGTGCCCAACCTTCATGTCATGAA
  GGCCATGCAGTCTCTCAAGTCCCGAGGCTACGTGAAGGAACAGTTTGCCTGGAGACATTTCTACTGGTAC
  CTTACCAATGAGGGTATCCAGTATCTCCGTGATTACCTTCATCTGCCCCCGGAGATTGTGCCTGCCACCC
  TACGCCGTAGCCGTCCAGAGACTGGCAGGCCTCGGCCTAAAGGTCTGGAGGGTGAGCGACCTGCGAGACT
  CACAAGAGGGGAAGCTGACAGAGATACCTACAGACGGAGTGCTGTGCCACCTGGTGCCGACAAGAAAGCC
  GAGGCTGGGGCTGGGTCAGCAACCGAATTCCAGTTTGTGCTACTCGTGAGCAGTAGTCGCCATCCAGACA
  GATGGATTGTCCCTGGAGGAGGCATGGAGCCCGAGGAGGAGCCAAGTGTGGCAGCAGTTCGTGAAGTCTG
  TGAGGAGGCTGGAGTAAAAGGGACATTGGGAAGATTAGTTGGAATTTTTGAGAACCAGGAGAGGAAGCAC
  AGGACGTATGTCTATGTGCTCATTGTCACTGAAGTGCTGGAAGACTGGGAAGATTCAGTTAACATTGGAA
  GGAAGAGGGAATGGTTTAAAATAGAAGACGCCATAAAAGTGCTGCAGTATCACAAACCCGTGCAGGCATC
  ATATTTTGAAACATTGAGGCAAGGCTACTCAGCCAACAATGGCACCCCAGTCGTGGCCACCACATACTCG
  GTTTCTGCTCAGAGCTCGATGTCAGGCATCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237231 representing NM_001202470
  Red=Cloning site Green=Tags(s)
  
  MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWY
  LTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKA
  EAGAGSATEFQFVLLVSSSRHPDRWIVPGGGMEPEEEPSVAAVREVCEEAGVKGTLGRLVGIFENQERKH
  RTYVYVLIVTEVLEDWEDSVNIGRKREWFKIEDAIKVLQYHKPVQASYFETLRQGYSANNGTPVVATTYS
  VSAQSSMSGIR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001202470
• ORF Size: 873 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001202470.1, NM_001202470.2, NP_001189399.1
• RefSeq Size: 2529
• RefSeq ORF: 876
• Locus ID: 100529239
• MW: 33.6 kDa
• Gene Summary: This locus represents naturally occurring read-through transcription between the neighboring RPS10 (ribosomal protein S10) and NUDT3 (nudix (nucleoside diphosphate linked moiety X)-type motif 3) genes on chromosome 6. The read-through transcript produces a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Feb 2011]