GLS2 (NM_001280798) Human Tagged ORF Clone

CAT#: RC237542

GLS2 (myc-DDK-tagged) - Human glutaminase 2 (liver, mitochondrial) (GLS2), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GLS2
• Synonyms: GA; GLS; hLGA; LGA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237542 representing NM_001280798
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAATGCTGGTGCCATTGTTGTCAGCTCCCTGATCAAGATGGACTGTAACAAAGCAGAGAAGTTTG
  ATTTTGTGTTGCAGTATCTCAACAAAATGGCTGGGAATGAATACATGGGTTTCAGCAATGCCACATTCCA
  GTCAGAGAAGGAAACAGGGGATCGGAATTATGCCATCGGCTATTATCTCAAGGAAAAGAAGTGCTTTCCT
  AAGGGGGTGGACATGATGGCTGCCCTTGATCTCTACTTCCAGCTGTGTTCTGTGGAGGTCACTTGTGAAT
  CAGGCAGTGTCATGGCAGCCACCCTCGCCAACGGTGGGATCTGCCCCATCACAGGCGAGAGTGTGCTGAG
  TGCTGAAGCAGTGCGCAACACCCTCAGCCTCATGCATTCCTGCGGCATGTATGACTTCTCTGGCCAGTTT
  GCCTTCCACGTGGGCCTGCCAGCCAAGTCAGCTGTATCAGGAGCCATCCTCCTGGTGGTACCCAATGTCA
  TGGGAATGATGTGCCTGTCACCCCCATTGGACAAGCTGGGGAACAGCCATAGGGGGACCAGCTTCTGCCA
  GAAGTTGGTGTCTCTCTTCAATTTCCACAACTATGACAACCTGAGGCACTGTGCTCGGAAGTTAGACCCA
  CGGCGTGAAGGGGCAGAAATTCGGAACAAGACTGTGGTCAACCTGTTATTTGCTGCCTATAGTGGCGATG
  TCTCAGCTCTTCGAAGGTTTGCCTTGTCAGCCATGGATATGGAACAGAAAGACTATGACTCGCGCACAGC
  TCTGCATGTTGCTGCAGCTGAAGGACACATCGAAGTTGTTAAATTCCTGATCGAGGCTTGCAAAGTGAAT
  CCTTTTGCCAAGGACAGGTGGGGCAACATTCCCCTGGATGATGCTGTGCAGTTCAACCATCTGGAGGTGG
  TCAAACTGCTTCAAGATTACCAGGACTCCTACACACTCTCTGAAACTCAGGCTGAGGCAGCAGCTGAGGC
  CCTGTCCAAAGAGAACTTAGAAAGCATGGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237542 representing NM_001280798
  Red=Cloning site Green=Tags(s)
  
  MVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEYMGFSNATFQSEKETGDRNYAIGYYLKEKKCFP
  KGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQF
  AFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGTSFCQKLVSLFNFHNYDNLRHCARKLDP
  RREGAEIRNKTVVNLLFAAYSGDVSALRRFALSAMDMEQKDYDSRTALHVAAAEGHIEVVKFLIEACKVN
  PFAKDRWGNIPLDDAVQFNHLEVVKLLQDYQDSYTLSETQAEAAAEALSKENLESMV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001280798
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001280798.1, NP_001267727.1
• RefSeq Size: 2296
• RefSeq ORF: 1014
• Locus ID: 27165
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism
• MW: 37.5 kDa
• Gene Summary: The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]