P2Y9 (LPAR4) (NM_001278000) Human Tagged ORF Clone

CAT#: RC237785

LPAR4 (myc-DDK-tagged) - Human lysophosphatidic acid receptor 4 (LPAR4), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LPAR4
• Synonyms: GPR23; LPA4; P2RY9; P2Y5-LIKE; P2Y9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237785 representing NM_001278000
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGACAGAAGATTCATTGACTTCCAATTCCAAGATTCAAATTCAAGCCTCAGACCCAGGTTGGGCA
  ATGCTACTGCCAATAATACTTGCATTGTTGATGATTCCTTCAAGTATAATCTCAATGGTGCTGTCTACAG
  TGTTGTATTCATCTTGGGTCTGATAACCAACAGTGTCTCTCTGTTTGTCTTCTGTTTCCGCATGAAAATG
  AGAAGTGAGACTGCTATTTTTATCACCAATCTAGCTGTCTCTGATTTGCTTTTTGTCTGTACACTACCTT
  TTAAAATATTTTACAACTTCAACCGCCACTGGCCTTTTGGTGACACCCTCTGCAAGATCTCTGGAACTGC
  ATTCCTTACCAACATCTATGGGAGCATGCTCTTTCTCACCTGTATTAGTGTGGATCGTTTCCTGGCCATT
  GTCTATCCTTTTCGATCTCGTACTATTAGGACTAGGAGGAATTCTGCCATTGTGTGTGCTGGTGTCTGGA
  TCCTAGTCCTCAGTGGCGGTATTTCAGCCTCTTTGTTTTCCACCACTAATGTCAACAATGCAACCACCAC
  CTGCTTTGAAGGCTTCTCCAAACGTGTCTGGAAGACTTATTTATCCAAGATCACAATATTTATTGAAGTT
  GTTGGGTTTATCATTCCTCTAATATTGAATGTCTCTTGCTCTTCTGTGGTGCTGAGAACTCTTCGCAAGC
  CTGCTACTCTGTCTCAAATTGGGACCAATAAGAAAAAAGTACTGAAAATGATCACAGTACATATGGCAGT
  CTTTGTGGTATGCTTTGTACCCTACAACTCTGTCCTCTTCTTGTATGCCCTGGTGCGCTCCCAAGCTATT
  ACTAATTGCTTTTTGGAAAGATTTGCAAAGATCATGTACCCAATCACCTTGTGCCTTGCAACTCTGAACT
  GTTGTTTTGACCCTTTCATCTATTACTTCACCCTTGAATCCTTTCAGAAGTCCTTCTACATCAATGCCCA
  CATCAGAATGGAGTCCCTGTTTAAGACTGAAACACCTTTGACCACAAAGCCTTCCCTTCCAGCTATTCAA
  GAGGAAGTGAGTGATCAAACAACAAATAATGGTGGTGAATTAATGCTAGAATCCACCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237785 representing NM_001278000
  Red=Cloning site Green=Tags(s)
  
  MGDRRFIDFQFQDSNSSLRPRLGNATANNTCIVDDSFKYNLNGAVYSVVFILGLITNSVSLFVFCFRMKM
  RSETAIFITNLAVSDLLFVCTLPFKIFYNFNRHWPFGDTLCKISGTAFLTNIYGSMLFLTCISVDRFLAI
  VYPFRSRTIRTRRNSAIVCAGVWILVLSGGISASLFSTTNVNNATTTCFEGFSKRVWKTYLSKITIFIEV
  VGFIIPLILNVSCSSVVLRTLRKPATLSQIGTNKKKVLKMITVHMAVFVVCFVPYNSVLFLYALVRSQAI
  TNCFLERFAKIMYPITLCLATLNCCFDPFIYYFTLESFQKSFYINAHIRMESLFKTETPLTTKPSLPAIQ
  EEVSDQTTNNGGELMLESTF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278000
• ORF Size: 1110 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278000.1, NP_001264929.1
• RefSeq Size: 2155 bp
• RefSeq ORF: 1113 bp
• Locus ID: 2846
• Cytogenetics: Xq21.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 41.9 kDa
• Gene Summary: 'This gene encodes a member of the lysophosphatidic acid receptor family. It may also be related to the P2Y receptors, a family of receptors that bind purine and pyrimidine nucleotides and are coupled to G proteins. The encoded protein may play a role in monocytic differentiation. [provided by RefSeq, Feb 2009]'