KCNK9 (NM_001282534) Human Tagged ORF Clone

CAT#: RC237820

KCNK9 (myc-DDK-tagged) - Human potassium channel, subfamily K, member 9 (KCNK9), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNK9
• Synonyms: K2p9.1; KT3.2; TASK-3; TASK3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237820 representing NM_001282534
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGGCAGAACGTGCGGACTCTGTCCCTCATCGTCTGCACCTTCACCTACCTGCTGGTGGGCGCCG
  CCGTGTTCGACGCCCTCGAGTCGGACCACGAGATGCGCGAGGAGGAGAAACTCAAAGCCGAGGAGATCCG
  GATCAAGGGGAAGTACAACATCAGCAGCGAGGACTACCGGCAGCTGGAGCTGGTGATCCTGCAGTCGGAA
  CCGCACCGCGCCGGCGTCCAGTGGAAATTCGCCGGCTCCTTCTACTTTGCGATCACGGTCATCACCACCA
  TAGGTTATGGGCACGCTGCACCTGGCACCGATGCGGGCAAGGCCTTCTGCATGTTCTACGCCGTGCTGGG
  CATCCCGCTGACACTGGTCATGTTCCAGAGCCTGGGCGAGCGCATGAACACCTTCGTGCGCTACCTGCTG
  AAGCGCATTAAGAAGTGCTGTGGCATGCGCAACACTGACGTGTCTATGGAGAACATGGTGACTGTGGGCT
  TCTTCTCCTGCATGGGGACGCTGTGCATCGGGGCGGCCGCCTTCTCCCAGTGTGAGGAGTGGAGCTTCTT
  CCACGCCTACTACTACTGCTTCATCACGTTGACTACCATTGGGTTCGGGGACTACGTGGCCCTGCAGACC
  AAGGGCGCCCTGCAGAAGAAGCCGCTCTACGTGGCCTTTAGCTTTATGTATATCCTGGTGGGGCTGACGG
  TCATCGGGGCCTTCCTCAACCTGGTCGTCCTCAGGTTCTTGACCATGAACAGTGAGGATGAGCGGCGGGA
  TGCTGAAGAGAGGGCATCCCTCGCCGGAAACCGCAACAGCATGGTCATTCACATCCCTGAGGAGCCGCGG
  CCCAGCCGGCCCAGGTACAAGGCGGACGTCCCGGACCTGCAGTCTGTGTGCTCCTGCACCTGCTACCGCT
  CGCAGGACTATGGCGGCCGCTCGGTGGCACCGCAGAACTCCTTCAGCGCCAAGCTTGCCCCCCACTACTT
  CCACTCCATCTCTTACAAGATCGAGGAGATCTCACCAAGCACATTAAAAAACAGCCTCTTCCCATCGCCT
  ATTAGCTCCATCTCTCCTGGGTTACACAGCTTTACCGACCACCAGAGGCTGATGAAACGCCGGAAGTCCG
  TT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237820 representing NM_001282534
  Red=Cloning site Green=Tags(s)
  
  MKRQNVRTLSLIVCTFTYLLVGAAVFDALESDHEMREEEKLKAEEIRIKGKYNISSEDYRQLELVILQSE
  PHRAGVQWKFAGSFYFAITVITTIGYGHAAPGTDAGKAFCMFYAVLGIPLTLVMFQSLGERMNTFVRYLL
  KRIKKCCGMRNTDVSMENMVTVGFFSCMGTLCIGAAAFSQCEEWSFFHAYYYCFITLTTIGFGDYVALQT
  KGALQKKPLYVAFSFMYILVGLTVIGAFLNLVVLRFLTMNSEDERRDAEERASLAGNRNSMVIHIPEEPR
  PSRPRYKADVPDLQSVCSCTCYRSQDYGGRSVAPQNSFSAKLAPHYFHSISYKIEEISPSTLKNSLFPSP
  ISSISPGLHSFTDHQRLMKRRKSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282534
• ORF Size: 1122 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282534.1, NP_001269463.1
• RefSeq Size: 2331 bp
• RefSeq ORF: 1125 bp
• Locus ID: 51305
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 42.3 kDa
• Gene Summary: This gene encodes a protein that contains multiple transmembrane regions and two pore-forming P domains and functions as a pH-dependent potassium channel. Amplification and overexpression of this gene have been observed in several types of human carcinomas. This gene is imprinted in the brain, with preferential expression from the maternal allele. A mutation in this gene was associated with Birk-Barel dysmorphism syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]