PPP2R5E (NM_001282181) Human Tagged ORF Clone

CAT#: RC237920

PPP2R5E (myc-DDK-tagged) - Human protein phosphatase 2, regulatory subunit B', epsilon isoform (PPP2R5E), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPP2R5E
• Synonyms: B56E; B56epsilon
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237920 representing NM_001282181
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACGCTATCTGATCTTAAAATGAAAGAATACAAGCGCTCCACTCTTAATGAACTGGTGGACTACA
  TTACAATAAGCAGAGGCTGTTTGACAGAGCAGACTTACCCTGAAGTAGTTAGAATGGTATCTTGCAATAT
  ATTCAGAACTCTCCCTCCTAGTGACAGCAATGAATTTGATCCAGAAGAAGATGAACCTACCCTTGAGGCA
  TCGTGGCCACACTTACAGCTTGTATATGAATTTTTCATACGATTTTTGGAAAGCCAAGAATTCCAACCCA
  GCATTGCCAAAAAATATATAGATCAGAAATTTGTATTACAGCTTCTGGAGCTATTTGACAGCGAAGACCC
  TCGGGAACGGGACTACTTAAAAACAGTCTTACACAGAATTTATGGCAAGTTTCTTGGTCTTAGAGCATTT
  ATCCGAAAACAGATTAACAATATTTTTCTAAGGTTTGTTTATGAAACAGAACACTTCAATGGTGTAGCTG
  AACTGCTGGAAATATTAGGAAGTATTATCAATGGCTTTGCTTTACCTCTTAAGGCAGAACACAAACAGTT
  TCTGGTGAAAGTATTGATCCCTTTACACACTGTCAGGAGCTTATCACTCTTCCATGCACAGCTGGCATAT
  TGTATAGTACAGTTTCTGGAGAAAGATCCTTCACTCACAGAACCAGTTATTAGGGGGTTAATGAAATTTT
  GGCCTAAAACATGTAGTCAAAAAGAGGTCATGTTCCTTGGGGAACTGGAAGAAATATTGGATGTGATTGA
  ACCTTCACAATTTGTTAAAATCCAAGAACCTTTGTTTAAACAAATCGCCAAGTGTGTATCTAGCCCCCAT
  TTTCAGGTGGCAGAAAGAGCACTCTATTATTGGAATAATGAATACATCATGAGTTTGATAGAAGAAAACT
  CTAACGTCATCCTTCCCATCATGTTTTCCAGCCTTTATAGGATTTCAAAAGAACATTGGAATCCGGCTAT
  TGTGGCGTTGGTGTACAATGTGTTGAAGGCATTTATGGAAATGAACAGCACCATGTTTGACGAGCTGACA
  GCCACATACAAGTCAGATCGTCAGCGTGAGAAAAAGAAAGAAAAGGAGCGTGAAGAATTGTGGAAAAAAT
  TGGAGGATCTGGAGTTAAAGAGAGGTCTTAGACGTGATGGAATAATTCCAACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237920 representing NM_001282181
  Red=Cloning site Green=Tags(s)
  
  MDTLSDLKMKEYKRSTLNELVDYITISRGCLTEQTYPEVVRMVSCNIFRTLPPSDSNEFDPEEDEPTLEA
  SWPHLQLVYEFFIRFLESQEFQPSIAKKYIDQKFVLQLLELFDSEDPRERDYLKTVLHRIYGKFLGLRAF
  IRKQINNIFLRFVYETEHFNGVAELLEILGSIINGFALPLKAEHKQFLVKVLIPLHTVRSLSLFHAQLAY
  CIVQFLEKDPSLTEPVIRGLMKFWPKTCSQKEVMFLGELEEILDVIEPSQFVKIQEPLFKQIAKCVSSPH
  FQVAERALYYWNNEYIMSLIEENSNVILPIMFSSLYRISKEHWNPAIVALVYNVLKAFMEMNSTMFDELT
  ATYKSDRQREKKKEKEREELWKKLEDLELKRGLRRDGIIPT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282181
• ORF Size: 1173 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282181.1, NP_001269110.1
• RefSeq Size: 3490 bp
• RefSeq ORF: 1176 bp
• Locus ID: 5529
• Cytogenetics: 14q23.2
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Oocyte meiosis, Wnt signaling pathway
• MW: 46.6 kDa
• Gene Summary: 'The protein encoded by this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]'