Lipoamide Dehydrogenase (DLD) (NM_001289750) Human Tagged ORF Clone

CAT#: RC238043

DLD (myc-DDK-tagged) - Human dihydrolipoamide dehydrogenase (DLD), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DLD
• Synonyms: DLDD; DLDH; E3; GCSL; LAD; PHE3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238043 representing NM_001289750
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCATGGAAAAGATTTTGCATCTAGAGGAATTGAAATGTCCGAAGTTCGCTTGAATTTAGACAAGA
  TGATGGAGCAGAAGAGTACTGCAGTAAAAGCTTTAACAGGTGGAATTGCCCACTTATTCAAACAGAATAA
  GGTTGTTCATGTCAATGGATATGGAAAGATAACTGGCAAAAATCAAGTCACTGCTACGAAAGCTGATGGC
  GGCACTCAGGTTATTGATACAAAGAACATTCTTATAGCCACGGGTTCAGAAGTTACTCCTTTTCCTGGAA
  TCACGATAGATGAAGATACAATAGTGTCATCTACAGGTGCTTTATCTTTAAAAAAAGTTCCAGAAAAGAT
  GGTTGTTATTGGTGCAGGAGTAATAGGTGTAGAATTGGGTTCAGTTTGGCAAAGACTTGGTGCAGATGTG
  ACAGCAGTTGAATTTTTAGGTCATGTAGGTGGAGTTGGAATTGATATGGAGATATCTAAAAACTTTCAAC
  GCATCCTTCAAAAACAGGGGTTTAAATTTAAATTGAATACAAAGGTTACTGGTGCTACCAAGAAGTCAGA
  TGGAAAAATTGATGTTTCTATTGAAGCTGCTTCTGGTGGTAAAGCTGAAGTTATCACTTGTGATGTACTC
  TTGGTTTGCATTGGCCGACGACCCTTTACTAAGAATTTGGGACTAGAAGAGCTGGGAATTGAACTAGATC
  CCAGAGGTAGAATTCCAGTCAATACCAGATTTCAAACTAAAATTCCAAATATCTATGCCATTGGTGATGT
  AGTTGCTGGTCCAATGCTGGCTCACAAAGCAGAGGATGAAGGCATTATCTGTGTTGAAGGAATGGCTGGT
  GGTGCTGTGCACATTGACTACAATTGTGTGCCATCAGTGATTTACACACACCCTGAAGTTGCTTGGGTTG
  GCAAATCAGAAGAGCAGTTGAAAGAAGAGGGTATTGAGTACAAAGTTGGGAAATTCCCATTTGCTGCTAA
  CAGCAGAGCTAAGACAAATGCTGACACAGATGGCATGGTGAAGATCCTTGGGCAGAAATCGACAGACAGA
  GTACTGGGAGCACATATTCTTGGACCAGGTGCTGGAGAAATGGTAAATGAAGCTGCTCTTGCTTTGGAAT
  ATGGAGCATCCTGTGAAGATATAGCTAGAGTCTGTCATGCACATCCGACCTTATCAGAAGCTTTTAGAGA
  AGCAAATCTTGCTGCGTCATTTGGCAAATCAATCAACTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238043 representing NM_001289750
  Red=Cloning site Green=Tags(s)
  
  MAHGKDFASRGIEMSEVRLNLDKMMEQKSTAVKALTGGIAHLFKQNKVVHVNGYGKITGKNQVTATKADG
  GTQVIDTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADV
  TAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGKAEVITCDVL
  LVCIGRRPFTKNLGLEELGIELDPRGRIPVNTRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAG
  GAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEYKVGKFPFAANSRAKTNADTDGMVKILGQKSTDR
  VLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAASFGKSINF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289750
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289750.1, NP_001276679.1
• RefSeq Size: 3464 bp
• RefSeq ORF: 1233 bp
• Locus ID: 1738
• Cytogenetics: 7q31.1
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Glycine, serine and threonine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine degradation
• MW: 44 kDa
• Gene Summary: 'This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]'