DYNC1LI2 (NM_001286157) Human Tagged ORF Clone

CAT#: RC238097

DYNC1LI2 (myc-DDK-tagged) - Human dynein, cytoplasmic 1, light intermediate chain 2 (DYNC1LI2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DYNC1LI2
• Synonyms: DNCLI2; LIC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238097 representing NM_001286157
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGGTGGGGGTGGAGAAGAAGCTGCTGCTAGGTCCCAACGGGCCCGCGGTGGCGGCCGCCGGCG
  ACCTGACCAGTGAGGAGGAGGAAGGCCAGAGCCTATGGTCCTCCATTCTGAGCGAAGTGTCCACCCGCGC
  CAGGTCCAAGCTGCCGTCCGGCAAGAACATCCTGGTCTTCGGTGAAGATGGTTCTGGTAAAACAACCCTC
  ATGACTAAACTACAAGGAGCTGAGCATGGCAAAAAAGGAAGAGGCCTAGAATATCTCTACCTCAGTGTCC
  ATGATGAGGACCGAGATGTTGTGAAAGATTTTCAAGACTATATGGAACCTGAAGAAGGTTGTCAAGGTTC
  CCCACAGAGAAGAGGCCCTCTGACCTCAGGCTCCGATGAAGAAAATGTTGCCCTGCCTCTGGGTGACAAT
  GTGCTGACTCATAACCTGGGGATCCCGGTGTTGGTGGTGTGCACAAAGTGTGATGCGGTGAGTGTCCTGG
  AGAAGGAGCACGATTACAGGGATGAGCATTTGGACTTTATCCAGTCACACCTGCGGAGGTTCTGCCTTCA
  GTATGGAGCTGCCTTGATTTACACATCAGTGAAAGAAGAGAAAAACCTCGACTTGTTGTATAAGTATATT
  GTTCATAAAACATACGGTTTCCACTTCACCACACCTGCCTTAGTTGTGGAAAAGGATGCCGTTTTTATAC
  CTGCAGGCTGGGACAATGAAAAGAAAATAGCTATTTTACATGAAAATTTTACAACCGTGAAGCCGGAAGA
  TGCATATGAAGACTTTATTGTGAAACCTCCCGTGAGAAAGCTGGTCCACGACAAAGAGTTGGCAGCAGAA
  GATGAGCAGGTGTTCCTAATGAAGCAACAGTCACTCCTTGCCAAGCAACCAGCCACTCCCACGAGAGCTT
  CTGAATCTCCTGCAAGAGGACCCTCTGGCTCTCCAAGGACCCAGGGTCGGGGAGGGCCAGCCAGTGTGCC
  TAGCTCCTCCCCAGGCACGTCAGTAAAAAAGCCGGACCCAAACATCAAAAATAATGCAGCAAGTGAAGGG
  GTGTTGGCCAGCTTCTTCAACAGTCTGTTGAGTAAAAAGACAGGCTCTCCTGGAAGTCCTGGTGCTGGTG
  GGGTGCAGAGCACAGCCAAGAAGTCAGGACAAAAGACTGTGTTGTCAAATGTTCAGGAAGAACTGGATAG
  AATGACTCGAAAGCCAGACTCTATGGTAACAAACTCTTCAACAGAAAATGAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238097 representing NM_001286157
  Red=Cloning site Green=Tags(s)
  
  MAPVGVEKKLLLGPNGPAVAAAGDLTSEEEEGQSLWSSILSEVSTRARSKLPSGKNILVFGEDGSGKTTL
  MTKLQGAEHGKKGRGLEYLYLSVHDEDRDVVKDFQDYMEPEEGCQGSPQRRGPLTSGSDEENVALPLGDN
  VLTHNLGIPVLVVCTKCDAVSVLEKEHDYRDEHLDFIQSHLRRFCLQYGAALIYTSVKEEKNLDLLYKYI
  VHKTYGFHFTTPALVVEKDAVFIPAGWDNEKKIAILHENFTTVKPEDAYEDFIVKPPVRKLVHDKELAAE
  DEQVFLMKQQSLLAKQPATPTRASESPARGPSGSPRTQGRGGPASVPSSSPGTSVKKPDPNIKNNAASEG
  VLASFFNSLLSKKTGSPGSPGAGGVQSTAKKSGQKTVLSNVQEELDRMTRKPDSMVTNSSTENEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286157
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286157.1, NP_001273086.1
• RefSeq Size: 4121 bp
• RefSeq ORF: 1248 bp
• Locus ID: 1783
• Cytogenetics: 16q22.1
• Protein Families: Druggable Genome
• MW: 45.5 kDa
• Gene Summary: 'Cytoplasmic dynein is a microtubule-associated motor protein (Hughes et al., 1995 [PubMed 7738094]). See DYNC1H1 (MIM 600112) for general information about dyneins.[supplied by OMIM, Mar 2008]'