Chimaerin 2 (CHN2) (NM_001293072) Human Tagged ORF Clone

CAT#: RC238312

CHN2 (myc-DDK-tagged) - Human chimerin 2 (CHN2), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CHN2
• Synonyms: ARHGAP3; BCH; CHN2-3; RHOGAP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238312 representing NM_001293072
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCTGAAGAATACCAGCCTCCTATATGGAAATCATACTTATATCAGTTACAGCAAGAGGCACCTC
  GTCCCAAGAGAATCATTTGTCCTCGGGAGGTGGAAAACAGACCAAAATATTATGGAAGAGAGTTTCATGG
  GATCATCTCTCGGGAGCAGGCGGATGAGCTTCTTGGAGGCGTGGAGGGTGCCTACATCCTTAGAGAAAGC
  CAGCGGCAACCAGGATGCTACACGCTGGCTCTCAGGTTTGGAAACCAGACCTTAAACTACAGGCTCTTCC
  ACGACGGGAAACACTTTGTGGGTGAGAAGAGGTTTGAGTCGATTCATGATCTGGTGACAGATGGCTTGAT
  AACACTGTACATAGAAACAAAAGCTGCCGAGTACATTTCAAAAATGACAACTAACCCCATCTATGAACAC
  ATTGGATATGCCACCCTACTCAGAGAAAAAGTATCCAGAAGGCTGAGCAGGTCTAAAAATGAACCAAGAA
  AAACAAACGTCACACATGAAGAACACACAGCGGTGGAAAAGATCTCCTCCCTGGTTCGAAGGGCTGCCCT
  CACACACAACGACAACCACTTCAATTATGAGAAGACACACAACTTTAAGGTCCACACGTTCCGAGGCCCA
  CACTGGTGTGAATATTGTGCCAATTTCATGTGGGGGCTCATCGCCCAAGGGGTCCGGTGCTCAGACTGTG
  GATTGAACGTACACAAACAGTGTTCCAAGCACGTTCCCAATGACTGCCAACCTGATCTCAAGAGGATCAA
  GAAAGTGTACTGTTGTGACCTCACAACACTTGTGAAGGCTCACAACACTCAGAGACCCATGGTGGTAGAC
  ATATGCATTCGGGAAATTGAAGCAAGAGGATTAAAATCGGAAGGCCTTTACAGAGTCTCTGGGTTCACTG
  AACACATTGAAGATGTCAAAATGGCATTTGACAGAGATGGTGAAAAGGCCGATATATCTGCCAATGTCTA
  TCCAGACATAAACATCATCACTGGAGCCCTTAAACTGTATTTCAGAGACTTACCCATCCCTGTCATCACA
  TATGATACCTATTCCAAATTTATAGATGCAGCAAAAATCTCCAATGCAGATGAGAGGCTGGAAGCCGTCC
  ATGAAGTGCTGATGCTGCTGCCTCCTGCCCACTATGAAACCCTCCGGTACCTAATGATCCACCTCAAAAA
  GGTTACTATGAATGAAAAAGACAATTTCATGAATGCAGAAAATCTGGGGATCGTGTTTGGGCCCACTCTG
  ATGAGGCCCCCTGAGGACAGCACCCTGACCACCCTGCATGATATGCGGTACCAAAAGCTGATTGTGCAGA
  TTTTAATAGAAAACGAAGACGTTTTATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238312 representing NM_001293072
  Red=Cloning site Green=Tags(s)
  
  MDAEEYQPPIWKSYLYQLQQEAPRPKRIICPREVENRPKYYGREFHGIISREQADELLGGVEGAYILRES
  QRQPGCYTLALRFGNQTLNYRLFHDGKHFVGEKRFESIHDLVTDGLITLYIETKAAEYISKMTTNPIYEH
  IGYATLLREKVSRRLSRSKNEPRKTNVTHEEHTAVEKISSLVRRAALTHNDNHFNYEKTHNFKVHTFRGP
  HWCEYCANFMWGLIAQGVRCSDCGLNVHKQCSKHVPNDCQPDLKRIKKVYCCDLTTLVKAHNTQRPMVVD
  ICIREIEARGLKSEGLYRVSGFTEHIEDVKMAFDRDGEKADISANVYPDINIITGALKLYFRDLPIPVIT
  YDTYSKFIDAAKISNADERLEAVHEVLMLLPPAHYETLRYLMIHLKKVTMNEKDNFMNAENLGIVFGPTL
  MRPPEDSTLTTLHDMRYQKLIVQILIENEDVLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293072
• ORF Size: 1359 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293072.1, NP_001280001.1
• RefSeq Size: 3218 bp
• RefSeq ORF: 1362 bp
• Locus ID: 1124
• Cytogenetics: 7p14.3
• MW: 53.1 kDa
• Gene Summary: 'This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that contains a phorbol-ester/diacylglycerol (DAG)-type zinc finger, a Rho-GAP domain, and an SH2 domain. The encoded protein translocates from the cytosol to the Golgi apparatus membrane upon binding by diacylglycerol (DAG). Activity of this protein is important in cell proliferation and migration, and expression changes in this gene have been detected in cancers. A mutation in this gene has also been associated with schizophrenia in men. Alternative transcript splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, May 2014]'