DCP1A (NM_001290206) Human Tagged ORF Clone

CAT#: RC238356

DCP1A (myc-DDK-tagged) - Human decapping mRNA 1A (DCP1A), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DCP1A
• Synonyms: HSA275986; Nbla00360; SMAD4IP1; SMIF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238356 representing NM_001290206
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGATGTGGTAGAAGAGGAGACACGGCGATCCCAGCAAGCTGCTCGGGACAAACAGAGTCCCAGCC
  AGGCCAATGGCTGCAGCGACCACAGGCCCATCGACATCCTGGAGATGCTGAGCAGAGCCAAGGATGAGTA
  TGAGAGGAATCAGATGGGTGACTCAAATATCTCCAGCCCTGGGTTACAGCCAAGCACTCAGCTCTCCAAT
  CTGGGAAGCACCGAGACTCTAGAAGAAATGCCCTCCGGGTCACAGGATAAGTCTGCTCCATCTGGACACA
  AGCATCTGACGGTAGAAGAGTTATTTGGAACCTCTTTGCCAAAGGAACAACCAGCAGTTGTGGGTCTGGA
  TTCAGAAGAAATGGAGAGGTTGCCAGGAGATGCCTCCCAGAAAGAGCCCAATTCATTCCTACCATTTCCC
  TTTGAGCAGTTAGGAGGAGCCCCTCAATCAGAAACCCTGGGTGTCCCTTCTGCTGCCCACCATTCAGTCC
  AGCCTGAAATCACCACCCCGGTGCTAATCACTCCAGCCTCCATCACACAGTCCAATGAAAAGCATGCTCC
  AACCTACACAATCCCGTTGAGCCCTGTTCTCAGTCCCACTCTGCCAGCTGAAGCTCCTACTGCACAGGTT
  CCCCCCAGCTTACCTCGAAACAGCACCATGATGCAGGCAGTGAAGACCACGCCTAGACAGAGGTCTCCAC
  TCCTGAACCAGCCAGTCCCTGAGCTAAGCCATGCCAGTCTGATTGCCAACCAGAGCCCCTTCAGGGCCCC
  ATTGAACGTGACGAACACAGCTGGCACATCCCTCCCAAGCGTTGATCTTCTCCAGAAACTCAGGTTGACC
  CCACAGCATGACCAAATACAGACACAACCACTTGGGAAAGGTGCAATGGTAGCCAGCTTTTCTCCGGCAG
  CTGGTCAGCTAGCCACACCTGAGAGCTTCATAGAGCCTCCCTCTAAGACAGCAGCAGCAAGAGTGGCGGC
  CTCAGCCTCCCTGAGCAACATGGTGCTTGCTCCCCTTCAGTCTATGCAGCAGAACCAGGATCCTGAAGTA
  TTTGTGCAGCCTAAGGTGTTATCCAGTGCCATCCCGGTTGCAGGCGCCCCACTGGTTACTGCAACGACCA
  CTGCAGTGTCTTCAGTCCTGCTGGCCCCAAGTGTTTTCCAGCAGACAGTTACAAGATCTTCGGACCTTGA
  GAGGAAAGCCAGCTCCCCTTCTCCTCTAACTATTGGAACGCCAGAAAGTCAGAGAAAGCCTTCCATTATT
  CTCAGCAAGTCTCAGCTCCAGGATACATTAATACATCTAATAAAGAATGATTCCAGCTTCCTCAGTACAC
  TTCATGAAGTCTACTTGCAGGTTCTGACCAAGAACAAAGACAACCACAACCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238356 representing NM_001290206
  Red=Cloning site Green=Tags(s)
  
  MADVVEEETRRSQQAARDKQSPSQANGCSDHRPIDILEMLSRAKDEYERNQMGDSNISSPGLQPSTQLSN
  LGSTETLEEMPSGSQDKSAPSGHKHLTVEELFGTSLPKEQPAVVGLDSEEMERLPGDASQKEPNSFLPFP
  FEQLGGAPQSETLGVPSAAHHSVQPEITTPVLITPASITQSNEKHAPTYTIPLSPVLSPTLPAEAPTAQV
  PPSLPRNSTMMQAVKTTPRQRSPLLNQPVPELSHASLIANQSPFRAPLNVTNTAGTSLPSVDLLQKLRLT
  PQHDQIQTQPLGKGAMVASFSPAAGQLATPESFIEPPSKTAAARVAASASLSNMVLAPLQSMQQNQDPEV
  FVQPKVLSSAIPVAGAPLVTATTTAVSSVLLAPSVFQQTVTRSSDLERKASSPSPLTIGTPESQRKPSII
  LSKSQLQDTLIHLIKNDSSFLSTLHEVYLQVLTKNKDNHNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290206
• ORF Size: 1383 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001290206.1, NP_001277135.1
• RefSeq Size: 5899
• RefSeq ORF: 1386
• Locus ID: 55802
• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation
• MW: 49.7 kDa
• Gene Summary: Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]