PNLIPRP1 (NM_001303135) Human Tagged ORF Clone

CAT#: RC238397

PNLIPRP1 (myc-DDK-tagged) - Human pancreatic lipase-related protein 1 (PNLIPRP1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PNLIPRP1
• Synonyms: PLRP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238397 representing NM_001303135
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGATCTTCTGGACAATCACACTTTTCCTGCTGGGAGCAGCCAAAGGAAAAGAAGTTTGCTATGAGG
  ACCTCGGGTGCTTTTCTGACACTGAGCCCTGGGGCGGGACAGCAATCAGGCCCCTGAAAATTCTCCCCTG
  GAGCCCTGAGAAGATCGGCACCCGCTTCCTGCTGTACACCAATGAAAACCCAAACAACTTTCAAATTCTC
  CTCCTCTCTGATCCATCAACAATTGAGGCATCAAATTTTCAAATGGACAGAAAGACCCGGTTCATCATCC
  ATGGCTTCATAGACAAAGGAGATGAGAGCTGGGTGACAGACATGTGCAAGAAACTGTTCGAGGTGGAGGA
  GGTGAACTGCATCTGCGTGGACTGGAAGAAGGGCTCCCAAGCCACCTACACACAGGCTGCCAACAACGTG
  CGAGTGGTGGGCGCCCAGGTGGCCCAGATGCTCGACATCCTCTTGACAGAGTATAGCTACCCCCCTTCCA
  AAGTTCACCTCATTGGCCACAGCCTGGGAGCCCACGTGGCTGGAGAGGCAGGAAGCAAGACTCCAGGCCT
  GAGCAGGATTACAGGGTTGGATCCTGTAGAAGCAAGTTTCGAGAGTACTCCTGAAGAGGTGCGACTTGAT
  CCCTCTGATGCTGACTTTGTTGATGTGATTCACACGGATGCAGCTCCCCTGATCCCATTCTTGGGTTTTG
  GAACGAACCAACAGATGGGTCATCTTGACTTCTTCCCCAATGGAGGAGAGAGCATGCCGGGATGCAAGAA
  GAATGCCCTGTCTCAGATCGTGGATCTAGATGGCATCTGGGCGGGAACCCGGGACTTTGTGGCTTGCAAT
  CACCTAAGAAGCTACAAGTATTACTTGGAAAGCATCCTCAATCCCGATGGGTTTGCTGCATATCCCTGCA
  CTTCCTACAAGTCCTTTGAGTCTGACAAGTGCTTCCCGTGTCCAGATCAAGGATGCCCACAGATGGGTCA
  CTATGCTGATAAATTTGCTGGCAGGACAAGTGAAGAGCAGCAGAAATTCTTCTTGAACACAGGAGAGGCT
  AGCAATTTCGCTCGCTGGAGATATGGGGTTTCCATCACACTGTCTGGAAGAACAGCCACTGGTCAGATCA
  AAGTTGCTTTGTTTGGAAATAAGGGAAACACTCACCAGTACAGTATCTTCAGGGGGATTCTCAAACCAGG
  CTCAACCCATTCCTATGAGTTTGATGCAAAGCTGGATGTTGGAACAATTGAGAAAGTCAAGTTTCTTTGG
  AATAACAATGTGATAAATCCAACCCTCCCCAAAGTGGGTGCCACCAAGATCACTGTGCAAAAGGGAGAAG
  AGAAGACAGTGTACAACTTCTGTAGCGAAGACACAGTGCGGGAAGACACGCTGCTCACCCTCACGCCCTG
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238397 representing NM_001303135
  Red=Cloning site Green=Tags(s)
  
  MLIFWTITLFLLGAAKGKEVCYEDLGCFSDTEPWGGTAIRPLKILPWSPEKIGTRFLLYTNENPNNFQIL
  LLSDPSTIEASNFQMDRKTRFIIHGFIDKGDESWVTDMCKKLFEVEEVNCICVDWKKGSQATYTQAANNV
  RVVGAQVAQMLDILLTEYSYPPSKVHLIGHSLGAHVAGEAGSKTPGLSRITGLDPVEASFESTPEEVRLD
  PSDADFVDVIHTDAAPLIPFLGFGTNQQMGHLDFFPNGGESMPGCKKNALSQIVDLDGIWAGTRDFVACN
  HLRSYKYYLESILNPDGFAAYPCTSYKSFESDKCFPCPDQGCPQMGHYADKFAGRTSEEQQKFFLNTGEA
  SNFARWRYGVSITLSGRTATGQIKVALFGNKGNTHQYSIFRGILKPGSTHSYEFDAKLDVGTIEKVKFLW
  NNNVINPTLPKVGATKITVQKGEEKTVYNFCSEDTVREDTLLTLTPC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001303135
• ORF Size: 1401 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001303135.1, NP_001290064.1
• RefSeq Size: 1513 bp
• RefSeq ORF: 1404 bp
• Locus ID: 5407
• Cytogenetics: 10q25.3
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways
• MW: 52.3 kDa
• Gene Summary: ''