Annexin A11 (ANXA11) (NM_001278409) Human Tagged ORF Clone

CAT#: RC238426

ANXA11 (myc-DDK-tagged) - Human annexin A11 (ANXA11), transcript variant f

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANXA11
• Synonyms: ALS23; ANX11; CAP-50; CAP50
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238426 representing NM_001278409
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCCCATCGGGCTGGATAACGTGGCCACCTATGCGGGGCAGTTCAACCAGGACTATCTCTCGGGAA
  TGGCGGCCAACATGTCTGGGACATTTGGAGGAGCCAACATGCCCAACCTGTACCCTGGGGCCCCTGGGGC
  TGGCTACCCACCAGTGCCCCCTGGCGGCTTTGGGCAGCCCCCCTCTGCCCAGCAGCCTGTTCCTCCCTAT
  GGGATGTATCCACCCCCAGGAGGAAACCCACCCTCCAGGATGCCCTCATATCCGCCATACCCAGGGGCCC
  CTGTGCCGGGCCAGCCCATGCCACCCCCCGGACAGCAGCCCCCAGGGGCCTACCCTGGGCAGCCACCAGT
  GACCTACCCTGGTCAGCCTCCAGTGCCACTCCCTGGGCAGCAGCAGCCAGTGCCGAGCTACCCAGGATAC
  CCGGGGTCTGGGACTGTCACCCCCGCTGTGCCCCCAACCCAGTTTGGAAGCCGAGGCACCATCACTGATG
  CTCCCGGCTTTGACCCCCTGCGAGATGCCGAGGTCCTGCGGAAGGCCATGAAAGGCTTCGGGACGGATGA
  GCAGGCCATCATTGACTGCCTGGGGAGTCGCTCCAACAAGCAGCGGCAGCAGATCCTACTTTCCTTCAAG
  ACGGCTTACGGCAAGGATTTGATCAAAGATCTGAAATCTGAACTGTCAGGAAACTTTGAGAAGACAATCT
  TGGCTCTGATGAAGACCCCAGTCCTCTTTGACATTTATGAGATAAAGGAAGCCATCAAGGGGGTTGGCAC
  TGATGAAGCCTGCCTGATTGAGATCCTCGCTTCCCGCAGCAATGAGCACATCCGAGAATTAAACAGAGCC
  TACAAAGCAGAATTCAAAAAGACCCTGGAAGAGGCCATTCGAAGCGACACATCAGGGCACTTCCAGCGGC
  TCCTCATCTCTCTCTCTCAGGGAAACCGTGATGAAAGCACAAACGTGGACATGTCACTCGCCCAGAGAGA
  TGCCCAGGAGCTGTATGCGGCCGGGGAGAACCGCCTGGGAACAGACGAGTCCAAGTTCAATGCGGTTCTG
  TGCTCCCGGAGCCGGGCCCACCTGGTAGCAGTTTTCAATGAGTACCAGAGAATGACAGGCCGGGACATTG
  AGAAGAGCATCTGCCGGGAGATGTCCGGGGACCTGGAGGAGGGCATGCTGGCCGTGGTGAAATGTCTCAA
  GAATACCCCAGCCTTCTTTGCGGAGAGGCTCAACAAGGCCATGAGGGGGGCAGGAACAAAGGACCGGACC
  CTGATTCGCATCATGGTGTCTCGCAGCGAGACCGACCTCCTGGACATCAGATCAGAGTATAAGCGGATGT
  ACGGCAAGTCGCTGTACCACGACATCTCGGGAGATACTTCAGGGGATTACCGGAAGATTCTGCTGAAGAT
  CTGTGGTGGCAATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238426 representing NM_001278409
  Red=Cloning site Green=Tags(s)
  
  MPPIGLDNVATYAGQFNQDYLSGMAANMSGTFGGANMPNLYPGAPGAGYPPVPPGGFGQPPSAQQPVPPY
  GMYPPPGGNPPSRMPSYPPYPGAPVPGQPMPPPGQQPPGAYPGQPPVTYPGQPPVPLPGQQQPVPSYPGY
  PGSGTVTPAVPPTQFGSRGTITDAPGFDPLRDAEVLRKAMKGFGTDEQAIIDCLGSRSNKQRQQILLSFK
  TAYGKDLIKDLKSELSGNFEKTILALMKTPVLFDIYEIKEAIKGVGTDEACLIEILASRSNEHIRELNRA
  YKAEFKKTLEEAIRSDTSGHFQRLLISLSQGNRDESTNVDMSLAQRDAQELYAAGENRLGTDESKFNAVL
  CSRSRAHLVAVFNEYQRMTGRDIEKSICREMSGDLEEGMLAVVKCLKNTPAFFAERLNKAMRGAGTKDRT
  LIRIMVSRSETDLLDIRSEYKRMYGKSLYHDISGDTSGDYRKILLKICGGND
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278409
• ORF Size: 1416 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278409.1, NP_001265338.1
• RefSeq Size: 2804 bp
• RefSeq ORF: 1419 bp
• Locus ID: 311
• Cytogenetics: 10q22.3
• MW: 51.7 kDa
• Gene Summary: 'This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Several transcript variants encoding two different isoforms have been identified. [provided by RefSeq, Dec 2015]'