SMARCA6 (HELLS) (NM_001289074) Human Tagged ORF Clone

CAT#: RC238451

HELLS (myc-DDK-tagged) - Human helicase, lymphoid-specific (HELLS), transcript variant 9

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HELLS
• Synonyms: ICF4; LSH; Nbla10143; PASG; SMARCA6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238451 representing NM_001289074
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTGCCGTCTAATCAGGGAGTTAAAACGATTCAATGCTGATAACAAACTTCTTTTGACTGGTACTC
  CCTTGCAAAACAATTTATCAGAACTTTGGTCATTGCTAAACTTTTTGTTGCCAGATGTATTTGATGACTT
  GAAAAGCTTTGAGTCTTGGTTTGACATCACTAGTCTTTCTGAAACTGCTGAAGATATTATTGCTAAAGAA
  AGAGAACAGAATGTATTGCATATGCTGCACCAGATTTTAACACCTTTCTTATTGAGAAGACTGAAGTCTG
  ATGTTGCTCTTGAAGTTCCTCCTAAACGAGAAGTAGTCGTTTATGCTCCACTTTCAAAGAAGCAGGAGAT
  CTTTTATACAGCCATTGTGAACCGTACAATTGCAAACATGTTTGGATCCAGTGAGAAAGAAACAATTGAG
  TTAAGTCCTACTGGTCGACCAAAACGACGAACTAGAAAATCAATAAATTACAGCAAAATAGATGATTTCC
  CTAATGAATTGGAAAAACTGATCAGTCAAATACAGCCAGAGGTGGACCGAGAAAGAGCTGTTGTGGAAGT
  GAATATCCCTGTAGAATCTGAAGTTAATCTGAAGCTGCAGAATATAATGATGCTACTTCGTAAATGTTGT
  AATCATCCATATTTGATTGAATATCCTATAGACCCTGTTACACAAGAATTTAAGATCGATGAAGAATTGG
  TAACAAATTCTGGGAAGTTCTTGATTTTGGATCGAATGCTGCCAGAACTAAAAAAAAGAGGTCACAAGGT
  GCTGCTTTTTTCACAAATGACAAGCATGTTGGACATTTTGATGGATTACTGCCATCTCAGAGATTTCAAC
  TTCAGCAGGCTTGATGGGTCCATGTCTTACTCAGAGAGAGAAAAAAACATGCACAGCTTCAACACGGATC
  CAGAGGTGTTTATCTTCTTAGTGAGTACACGAGCTGGTGGCCTGGGCATTAATCTGACTGCAGCAGATAC
  AGTTATCATTTATGATAGTGATTGGAACCCCCAGTCGGATCTTCAGGCCCAGGATAGATGTCATAGAATT
  GGTCAGACAAAGCCAGTTGTTGTTTATCGCCTTGTTACAGCAAATACTATCGATCAGAAAATTGTGGAAA
  GAGCAGCTGCTAAAAGGAAACTGGAAAAGTTGATCATCCATAAAAATCATTTCAAAGGTGGTCAGTCTGG
  ATTAAATCTGTCTAAGAATTTCTTAGATCCTAAGGAATTAATGGAATTATTAAAATCTAGAGATTATGAA
  AGGGAAATAAAAGGATCAAGAGAGAAGGTCATTAGTGATAAAGATCTAGAGTTGTTGTTAGATCGAAGTG
  ATCTTATTGATCAAATGAATGCTTCAGGACCAATTAAAGAGAAGATGGGGATATTCAAGATATTAGAAAA
  TTCTGAAGATTCCAGTCCTGAATGTTTGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238451 representing NM_001289074
  Red=Cloning site Green=Tags(s)
  
  MKCRLIRELKRFNADNKLLLTGTPLQNNLSELWSLLNFLLPDVFDDLKSFESWFDITSLSETAEDIIAKE
  REQNVLHMLHQILTPFLLRRLKSDVALEVPPKREVVVYAPLSKKQEIFYTAIVNRTIANMFGSSEKETIE
  LSPTGRPKRRTRKSINYSKIDDFPNELEKLISQIQPEVDRERAVVEVNIPVESEVNLKLQNIMMLLRKCC
  NHPYLIEYPIDPVTQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLLFSQMTSMLDILMDYCHLRDFN
  FSRLDGSMSYSEREKNMHSFNTDPEVFIFLVSTRAGGLGINLTAADTVIIYDSDWNPQSDLQAQDRCHRI
  GQTKPVVVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLNLSKNFLDPKELMELLKSRDYE
  REIKGSREKVISDKDLELLLDRSDLIDQMNASGPIKEKMGIFKILENSEDSSPECLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289074
• ORF Size: 1431 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289074.1, NP_001276003.1
• RefSeq Size: 3082 bp
• RefSeq ORF: 1434 bp
• Locus ID: 3070
• Cytogenetics: 10q23.33
• MW: 55.6 kDa
• Gene Summary: 'This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2014]'