COP1 (RFWD2) (NM_001286644) Human Tagged ORF Clone

CAT#: RC238538

RFWD2 (myc-DDK-tagged) - Human ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RFWD2
• Synonyms: RFWD2; RNF200
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238538 representing NM_001286644
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTGGACAATATTGACCATCTGTATCCTAATTTCTTGGGAATCACATGCAGCCCAACTACAGATTC
  TTATGGAATTCCTCAAGGTTGCAAGAAGAAATAAGAGAGAGCAACTGGAACAGATCCAGAAGGAGCTAAG
  TGTTTTGGAAGAGGATATTAAGAGAGTGGAAGAAATGAGTGGCTTATACTCTCCTGTCAGTGAGGATAGC
  ACAGTGCCTCAATTTGAAGCTCCTTCTCCATCACACAGTAGTATTATTGATTCCACAGAATACAGCCAAC
  CTCCAGGTTTCAGTGGCAGTTCTCAGACAAAGAAACAGCCTTGGTATAATAGCACGTTAGCATCAAGACG
  AAAACGACTTACTGCTCATTTTGAAGACTTGGAGCAGTGTTACTTTTCTACAAGGATGTCTCGTATCTCA
  GATGACAGTCGAACTGCAAGCCAGTTGGATGAATTTCAGGAATGCTTGTCCAAGTTTACTCGATATAATT
  CAGTACGACCTTTAGCCACATTGTCATATGCTAGTGATCTCTATAATGGTTCCAGTATAGTCTCTAGTAT
  TGAATTTGACCGGGATTGTGACTATTTTGCGATTGCTGGAGTTACAAAGAAGATTAAAGTCTATGAATAT
  GACACTGTCATCCAGGATGCAGTGGATATTCATTACCCTGAGAATGAAATGACCTGCAATTCGAAAATCA
  GCTGTATCAGTTGGAGTAGTTACCATAAGAACCTGTTAGCTAGCAGTGATTATGAAGGCACTGTTATTTT
  ATGGGATGGATTCACAGGACAGAGGTCAAAGGTCTATCAGGAGCATGAGAAGAGGTGTTGGAGTGTTGAC
  TTTAATTTGATGGATCCTAAACTCTTGGCTTCAGGTTCTGATGATGCAAAAGTGAAGCTGTGGTCTACCA
  ATCTAGACAACTCAGTGGCAAGCATTGAGGCAAAGGCTAATGTGTGCTGTGTTAAATTCAGCCCCTCTTC
  CAGATACCATTTGGCTTTCGGCTGTGCAGATCACTGTGTCCACTACTATGATCTTCGTAACACTAAACAG
  CCAATCATGGTATTCAAAGGACACCGTAAAGCAGTCTCTTATGCAAAGTTTGTGAGTGGTGAGGAAATTG
  TCTCTGCCTCAACAGACAGTCAGCTAAAACTGTGGAATGTAGGGAAACCATACTGCCTACGTTCCTTCAA
  GGGTCATATCAATGAAAAAAACTTTGTAGGCCTGGCTTCCAATGGAGATTATATAGCTTGTGGAAGTGAA
  AATAACTCTCTCTACCTGTACTATAAAGGACTTTCTAAGACTTTGCTAACTTTTAAGTTTGATACAGTCA
  AAAGTGTTCTCGACAAAGACCGAAAAGAAGATGATACAAATGAATTTGTTAGTGCTGTGTGCTGGAGGGC
  ACTACCAGATGGGGAGTCCAATGTGCTGATTGCTGCTAACAGTCAGGGTACAATTAAGGTGCTAGAATTG
  GTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238538 representing NM_001286644
  Red=Cloning site Green=Tags(s)
  
  MLWTILTICILISWESHAAQLQILMEFLKVARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDS
  TVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRIS
  DDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEY
  DTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVD
  FNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQ
  PIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSE
  NNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLEL
  V
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286644
• ORF Size: 1473 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001286644.1, NP_001273573.1
• RefSeq Size: 2609
• RefSeq ORF: 1476
• Locus ID: 64326
• Protein Pathways: p53 signaling pathway, Ubiquitin mediated proteolysis
• MW: 56.1 kDa