ELF2 (NM_001276459) Human Tagged ORF Clone

CAT#: RC238542

ELF2 (myc-DDK-tagged) - Human E74-like factor 2 (ets domain transcription factor) (ELF2), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ELF2
• Synonyms: b; EU32; NERF; NERF-1A; NERF-1a; NERF-1B; NERF-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238542 representing NM_001276459
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACGTCTCTGCATGAGGGACCCACGAACCAGCTGGATCTGCTCATCCGGGCCGTGGAAGCATCAG
  TTCACAGCAGTAATGCACACTGTACAGATAAGACAATTGAAGCTGCTGAAGCCCTGCTTCATATGGAATC
  TCCTACCTGCTTGAGGGATTCAAGAAGTCCTGAATTCATCCATGCTGCTATGAGGCCAGATGTCATTACA
  GAAACTGTAGTGGAGGTGTCAACTGAAGAGTCTGAACCCATGGATACCTCTCCTATTCCAACATCACCAG
  ATAGCCATGAACCAATGAAAAAGAAAAAAGGAAACACAACCTATTTGTGGGAGTTTCTTTTAGATCTACT
  TCAAGATAAAAATACTTGTCCCAGGTATATTAAATGGACTCAGAGAGAAAAAGGCATATTCAAGCTGGTG
  GATTCAAAGGCTGTCTCTAAGCTTTGGGGAAAGCATAAGAACAAACCAGACATGAACTATGAAACCATGG
  GACGAGCTTTGAGATACTACTACCAAAGGGGAATTCTTGCAAAGGTTGAAGGACAGAGGCTTGTATATCA
  GTTCAAGGATATGCCGAAAAACATAGTGGTCATAGATGATGACAAAAGTGAAACCTGTAATGAAGATTTA
  GCAGGAACTACTGATGAAAAATCATTAGAACGAGTGTCACTGTCTGCAGAAAGTCTCCTGAAAGCAGCAT
  CCTCTGTTCGCAGTGGAAAAAATTCATCCCCTATAAACTGCTCCAGAGCAGAGAAGGGTGTAGCTAGAGT
  TGTGAATATCACTTCCCCTGGGCACGATGCTTCATCCAGGTCTCCTACTACCACTGCATCTGTGTCAGCA
  ACAGCAGCTCCAAGGACAGTTCGTGTGGCAATGCAGGTACCTGTTGTAATGACATCATTGGGTCAGAAAA
  TTTCAACTGTGGCAGTTCAGTCAGTTAATGCAGGTGCACCATTAATAACCAGCACTAGTCCAACAACAGC
  GACCTCTCCAAAGGTAGTCATTCAGACAATCCCTACTGTGATGCCAGCTTCTACTGAAAATGGAGACAAA
  ATCACCATGCAGCCTGCCAAAATTATTACCATCCCAGCTACACAGCTTGCACAGTGTCAACTGCAGACAA
  AGTCAAATCTGACTGGATCAGGAAGCATTAACATTGTTGGAACCCCATTGGCTGTGAGAGCACTTACCCC
  TGTTTCAATAGCCCATGGTACACCTGTAATGAGACTATCAATGCCTACTCAGCAGGCATCTGGCCAGACT
  CCTCCTCGAGTTATCAGTGCAGTCATAAAGGGGCCAGAGGTTAAATCGGAAGCAGTGGCAAAAAAGCAAG
  AACATGATGTGAAAACTTTGCAGCTAGTAGAAGAAAAACCAGCAGATGGAAATAAGACAGTGACCCACGT
  AGTGGTTGTCAGTGCGCCTTCAGCTATTGCCCTTCCTGTAACTATGAAAACAGAAGGACTAGTGACATGT
  GAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238542 representing NM_001276459
  Red=Cloning site Green=Tags(s)
  
  MATSLHEGPTNQLDLLIRAVEASVHSSNAHCTDKTIEAAEALLHMESPTCLRDSRSPEFIHAAMRPDVIT
  ETVVEVSTEESEPMDTSPIPTSPDSHEPMKKKKGNTTYLWEFLLDLLQDKNTCPRYIKWTQREKGIFKLV
  DSKAVSKLWGKHKNKPDMNYETMGRALRYYYQRGILAKVEGQRLVYQFKDMPKNIVVIDDDKSETCNEDL
  AGTTDEKSLERVSLSAESLLKAASSVRSGKNSSPINCSRAEKGVARVVNITSPGHDASSRSPTTTASVSA
  TAAPRTVRVAMQVPVVMTSLGQKISTVAVQSVNAGAPLITSTSPTTATSPKVVIQTIPTVMPASTENGDK
  ITMQPAKIITIPATQLAQCQLQTKSNLTGSGSINIVGTPLAVRALTPVSIAHGTPVMRLSMPTQQASGQT
  PPRVISAVIKGPEVKSEAVAKKQEHDVKTLQLVEEKPADGNKTVTHVVVVSAPSAIALPVTMKTEGLVTC
  EK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276459
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276459.1, NP_001263388.1
• RefSeq Size: 2969 bp
• RefSeq ORF: 1479 bp
• Locus ID: 1998
• Cytogenetics: 4q31.1
• Protein Families: Druggable Genome, Transcription Factors
• MW: 53.5 kDa
• Gene Summary: ''