DCP1A (NM_001290205) Human Tagged ORF Clone

CAT#: RC238638

DCP1A (myc-DDK-tagged) - Human decapping mRNA 1A (DCP1A), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DCP1A
• Synonyms: HSA275986; Nbla00360; SMAD4IP1; SMIF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238638 representing NM_001290205
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCATTTCTTCTGTATAGAAATGCAAGCTGTTGAGAAAACAAGAGGCTCAGAGCTGTTTAGGAATC
  TGCTCGAGATTCGCAAAGTGTCGATATATAGTATCTGGTTTTATGACAAGAATGACTGTCACCGCATAGC
  AAAACTCATGGCTGATGTGGTAGAAGAGGAGACACGGCGATCCCAGCAAGCTGCTCGGGACAAACAGAGT
  CCCAGCCAGGCCAATGGCTGCAGCGACCACAGGCCCATCGACATCCTGGAGATGCTGAGCAGAGCCAAGG
  ATGAGTATGAGAGGAATCAGATGGGTGACTCAAATATCTCCAGCCCTGGGTTACAGCCAAGCACTCAGCT
  CTCCAATCTGGGAAGCACCGAGACTCTAGAAGAAATGCCCTCCGGGTCACAGGATAAGTCTGCTCCATCT
  GGACACAAGCATCTGACGGTAGAAGAGTTATTTGGAACCTCTTTGCCAAAGGAACAACCAGCAGTTGTGG
  GTCTGGATTCAGAAGAAATGGAGAGGTTGCCAGGAGATGCCTCCCAGAAAGAGCCCAATTCATTCCTACC
  ATTTCCCTTTGAGCAGTTAGGAGGAGCCCCTCAATCAGAAACCCTGGGTGTCCCTTCTGCTGCCCACCAT
  TCAGTCCAGCCTGAAATCACCACCCCGGTGCTAATCACTCCAGCCTCCATCACACAGTCCAATGAAAAGC
  ATGCTCCAACCTACACAATCCCGTTGAGCCCTGTTCTCAGTCCCACTCTGCCAGCTGAAGCTCCTACTGC
  ACAGGTTCCCCCCAGCTTACCTCGAAACAGCACCATGATGCAGGCAGTGAAGACCACGCCTAGACAGAGG
  TCTCCACTCCTGAACCAGCCAGTCCCTGAGCTAAGCCATGCCAGTCTGATTGCCAACCAGAGCCCCTTCA
  GGGCCCCATTGAACGTGACGAACACAGCTGGCACATCCCTCCCAAGCGTTGATCTTCTCCAGAAACTCAG
  GTTGACCCCACAGCATGACCAAATACAGACACAACCACTTGGGAAAGGTGCAATGGTAGCCAGCTTTTCT
  CCGGCAGCTGGTCAGCTAGCCACACCTGAGAGCTTCATAGAGCCTCCCTCTAAGACAGCAGCAGCAAGAG
  TGGCGGCCTCAGCCTCCCTGAGCAACATGGTGCTTGCTCCCCTTCAGTCTATGCAGCAGAACCAGGATCC
  TGAAGTATTTGTGCAGCCTAAGGTGTTATCCAGTGCCATCCCGGTTGCAGGCGCCCCACTGGTTACTGCA
  ACGACCACTGCAGTGTCTTCAGTCCTGCTGGCCCCAAGTGTTTTCCAGCAGACAGTTACAAGATCTTCGG
  ACCTTGAGAGGAAAGCCAGCTCCCCTTCTCCTCTAACTATTGGAACGCCAGAAAGTCAGAGAAAGCCTTC
  CATTATTCTCAGCAAGTCTCAGCTCCAGGATACATTAATACATCTAATAAAGAATGATTCCAGCTTCCTC
  AGTACACTTCATGAAGTCTACTTGCAGGTTCTGACCAAGAACAAAGACAACCACAACCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238638 representing NM_001290205
  Red=Cloning site Green=Tags(s)
  
  MNHFFCIEMQAVEKTRGSELFRNLLEIRKVSIYSIWFYDKNDCHRIAKLMADVVEEETRRSQQAARDKQS
  PSQANGCSDHRPIDILEMLSRAKDEYERNQMGDSNISSPGLQPSTQLSNLGSTETLEEMPSGSQDKSAPS
  GHKHLTVEELFGTSLPKEQPAVVGLDSEEMERLPGDASQKEPNSFLPFPFEQLGGAPQSETLGVPSAAHH
  SVQPEITTPVLITPASITQSNEKHAPTYTIPLSPVLSPTLPAEAPTAQVPPSLPRNSTMMQAVKTTPRQR
  SPLLNQPVPELSHASLIANQSPFRAPLNVTNTAGTSLPSVDLLQKLRLTPQHDQIQTQPLGKGAMVASFS
  PAAGQLATPESFIEPPSKTAAARVAASASLSNMVLAPLQSMQQNQDPEVFVQPKVLSSAIPVAGAPLVTA
  TTTAVSSVLLAPSVFQQTVTRSSDLERKASSPSPLTIGTPESQRKPSIILSKSQLQDTLIHLIKNDSSFL
  STLHEVYLQVLTKNKDNHNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290205
• ORF Size: 1530 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001290205.1, NP_001277134.1
• RefSeq Size: 6082
• RefSeq ORF: 1533
• Locus ID: 55802
• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation
• MW: 55.7 kDa
• Gene Summary: Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]