ENTPD3 (NM_001291960) Human Tagged ORF Clone

CAT#: RC238734

ENTPD3 (myc-DDK-tagged) - Human ectonucleoside triphosphate diphosphohydrolase 3 (ENTPD3), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ENTPD3
• Synonyms: CD39L3; HB6; NTPDase-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238734 representing NM_001291960
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCACTGTGCTGACCCGCCAACCATGTGAGCAAGCAGGCCTCAAGGCCCTCTACCGAACTCCAACCA
  TCATTGCCTTGGTGGTCTTGCTTGTGAGTATTGTGGTACTTGTGAGTATCACTGTCATCCAGATCCACAA
  GCAAGAGGTCCTCCCTCCAGGACTGAAGTATGGTATTGTGCTGGATGCCGGGTCTTCAAGAACCACAGTC
  TACGTGTATCAATGGCCAGCAGAAAAAGAGAATAATACCGGAGTGGTCAGTCAAACCTTCAAATGTAGTG
  TGAAAGGCTCTGGAATCTCCAGCTATGGAAATAACCCCCAAGATGTCCCCAGAGCCTTTGAGGAGTGTAT
  GCAAAAAGTCAAGGGGCAGGTTCCATCCCACCTCCACGGATCCACCCCCATTCACCTGGGAGCCACGGCT
  GGGATGCGCTTGCTGAGGTTGCAAAATGAAACAGCAGCTAATGAAGTCCTTGAAAGCATCCAAAGCTACT
  TCAAGTCCCAGCCCTTTGACTTTAGGGGTGCTCAAATCATTTCTGGGCAAGAAGAAGGGGTATATGGATG
  GATTACAGCCAACTATTTAATGGGAAATTTCCTGGAGAAGAACCTGTGGCACATGTGGGTGCACCCGCAT
  GGAGTGGAAACCACGGGTGCCCTGGACTTAGGTGGTGCCTCCACCCAAATATCCTTCGTGGCAGGAGAGA
  AGATGGATCTGAACACCAGCGACATCATGCAGGTGTCCCTGTATGGCTACGTATACACGCTCTACACACA
  CAGCTTCCAGTGCTATGGCCGGAATGAGGCTGAGAAGAAGTTTCTGGCAATGCTCCTGCAGAATTCTCCT
  ACCAAAAACCATCTCACCAATCCCTGTTACCCTCGGGATTATAGCATCAGCTTCACCATGGGCCATGTAT
  TTGATAGCCTGTGCACTGTGGACCAGAGGCCAGAAAGTTATAACCCCAATGATGTCATCACTTTTGAAGG
  AACTGGGGACCCATCTCTGTGTAAGGAGAAGGTGGCTTCCATATTTGACTTCAAAGCTTGCCATGATCAA
  GAAACCTGTTCTTTTGATGGGGTTTATCAGCCAAAGATTAAAGGGCCATTTGTGGCTTTTGCAGGATTCT
  ACTACACAGCCAGTGCTTTAAATCTTTCAGGTAGCTTTTCCCTGGACACCTTCAACTCCAGCACCTGGAA
  TTTCTGCTCACAGAATTGGAGTCAGCTCCCACTGCTGCTCCCCAAATTTGATGAGGTATATGCCCGCTCT
  TACTGCTTCTCAGCCAACTACATCTACCACTTGTTTGTGAACGGTTACAAATTCACAGAGGAGACTTGGC
  CCCAAATACACTTTGAAAAAGAAGTGGGGAATAGCAGCATAGCCTGGTCTCTTGGCTACATGCTCAGCCT
  GACCAACCAGATCCCAGCTGAAAGCCCTCTGATCCGTCTGCCCATAGAACCACCTGTCTTTGTGGGCACC
  CTCGCTTTCTTCACAGCGGCAGCCTTGCTGTGTCTGGCATTTCTTGCATACCTGTGTTCAGCAACCAGAA
  GAAAGAGGCACTCCGAGCATGCCTTTGACCATGCAGTGGATTCTGAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238734 representing NM_001291960
  Red=Cloning site Green=Tags(s)
  
  MFTVLTRQPCEQAGLKALYRTPTIIALVVLLVSIVVLVSITVIQIHKQEVLPPGLKYGIVLDAGSSRTTV
  YVYQWPAEKENNTGVVSQTFKCSVKGSGISSYGNNPQDVPRAFEECMQKVKGQVPSHLHGSTPIHLGATA
  GMRLLRLQNETAANEVLESIQSYFKSQPFDFRGAQIISGQEEGVYGWITANYLMGNFLEKNLWHMWVHPH
  GVETTGALDLGGASTQISFVAGEKMDLNTSDIMQVSLYGYVYTLYTHSFQCYGRNEAEKKFLAMLLQNSP
  TKNHLTNPCYPRDYSISFTMGHVFDSLCTVDQRPESYNPNDVITFEGTGDPSLCKEKVASIFDFKACHDQ
  ETCSFDGVYQPKIKGPFVAFAGFYYTASALNLSGSFSLDTFNSSTWNFCSQNWSQLPLLLPKFDEVYARS
  YCFSANYIYHLFVNGYKFTEETWPQIHFEKEVGNSSIAWSLGYMLSLTNQIPAESPLIRLPIEPPVFVGT
  LAFFTAAALLCLAFLAYLCSATRRKRHSEHAFDHAVDSD
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291960
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291960.1, NP_001278889.1
• RefSeq Size: 2867 bp
• RefSeq ORF: 1590 bp
• Locus ID: 956
• Cytogenetics: 3p22.1
• Protein Families: Transmembrane
• Protein Pathways: Purine metabolism, Pyrimidine metabolism
• MW: 59.6 kDa
• Gene Summary: 'This gene encodes a plasma membrane-bound divalent cation-dependent E-type nucleotidase. The encoded protein is involved in the regulation of extracellular levels of ATP by hydrolysis of it and other nucleotides. Multiple transcript variants have been described. [provided by RefSeq, May 2014]'