GALNT2 (NM_001291866) Human Tagged ORF Clone

CAT#: RC238757

GALNT2 (myc-DDK-tagged) - Human polypeptide N-acetylgalactosaminyltransferase 2 (GALNT2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GALNT2
• Synonyms: GalNAc-T2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238757 representing NM_001291866
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACAGAGAGGACTGGAATGAAATTGACCCCATTAAAAAGAAAGACCTTCATCACAGCAATGGAG
  AAGAGAAAGCACAAAGCATGGAGACCCTCCCTCCAGGGAAAGTACGGTGGCCAGACTTTAACCAGGAAGC
  TTATGTTGGAGGGACGATGGTCCGCTCCGGGCAGGACCCTTACGCCCGCAACAAGTTCAACCAGGTGGAG
  AGTGATAAGCTTCGAATGGACAGAGCCATCCCTGACACCCGGCATGACCAGTGTCAGCGGAAGCAGTGGC
  GGGTGGATCTGCCGGCCACCAGCGTGGTGATCACGTTTCACAATGAAGCCAGGTCGGCCCTACTCAGGAC
  CGTGGTCAGCGTGCTTAAGAAAAGCCCGCCCCATCTCATAAAAGAAATCATCTTGGTGGATGACTACAGC
  AATGATCCTGAGGACGGGGCTCTCTTGGGGAAAATTGAGAAAGTGCGAGTTCTTAGAAATGATCGACGAG
  AAGGCCTCATGCGCTCACGGGTTCGGGGGGCCGATGCTGCCCAAGCCAAGGTCCTGACCTTCCTGGACAG
  TCACTGCGAGTGTAATGAGCACTGGCTGGAGCCCCTCCTGGAAAGGGTGGCGGAGGACAGGACTCGGGTT
  GTGTCACCCATCATCGATGTCATTAATATGGACAACTTTCAGTATGTGGGGGCATCTGCTGACTTGAAGG
  GCGGTTTTGATTGGAACTTGGTATTCAAGTGGGATTACATGACGCCTGAGCAGAGAAGGTCCCGGCAGGG
  GAACCCAGTCGCCCCTATAAAAACCCCCATGATTGCTGGTGGGCTGTTTGTGATGGATAAGTTCTATTTT
  GAAGAACTGGGGAAGTACGACATGATGATGGATGTGTGGGGAGGAGAGAACCTAGAGATCTCGTTCCGCG
  TGTGGCAGTGTGGTGGCAGCCTGGAGATCATCCCGTGCAGCCGTGTGGGACACGTGTTCCGGAAGCAGCA
  CCCCTACACGTTCCCGGGTGGCAGTGGCACTGTCTTTGCCCGAAACACCCGCCGGGCAGCAGAGGTCTGG
  ATGGATGAATACAAAAATTTCTATTATGCAGCAGTGCCTTCTGCTAGAAACGTTCCTTATGGAAATATTC
  AGAGCAGATTGGAGCTTAGGAAGAAACTCAGCTGCAAGCCTTTCAAATGGTACCTTGAAAATGTCTATCC
  AGAGTTAAGGGTTCCAGACCATCAGGATATAGCTTTTGGGGCCTTGCAGCAGGGAACTAACTGCCTCGAC
  ACTTTGGGACACTTTGCTGATGGTGTGGTTGGAGTTTATGAATGTCACAATGCTGGGGGAAACCAGGAAT
  GGGCCTTGACGAAGGAGAAGTCGGTGAAGCACATGGATTTGTGCCTTACTGTGGTGGACCGGGCACCGGG
  CTCTCTTATAAAGCTGCAGGGCTGCCGAGAAAATGACAGCAGACAGAAATGGGAACAGATCGAGGGCAAC
  TCCAAGCTGAGGCACGTGGGCAGCAACCTGTGCCTGGACAGTCGCACGGCCAAGAGCGGGGGCCTAAGCG
  TGGAGGTGTGTGGCCCGGCCCTTTCGCAGCAGTGGAAGTTCACGCTCAACCTGCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238757 representing NM_001291866
  Red=Cloning site Green=Tags(s)
  
  MADREDWNEIDPIKKKDLHHSNGEEKAQSMETLPPGKVRWPDFNQEAYVGGTMVRSGQDPYARNKFNQVE
  SDKLRMDRAIPDTRHDQCQRKQWRVDLPATSVVITFHNEARSALLRTVVSVLKKSPPHLIKEIILVDDYS
  NDPEDGALLGKIEKVRVLRNDRREGLMRSRVRGADAAQAKVLTFLDSHCECNEHWLEPLLERVAEDRTRV
  VSPIIDVINMDNFQYVGASADLKGGFDWNLVFKWDYMTPEQRRSRQGNPVAPIKTPMIAGGLFVMDKFYF
  EELGKYDMMMDVWGGENLEISFRVWQCGGSLEIIPCSRVGHVFRKQHPYTFPGGSGTVFARNTRRAAEVW
  MDEYKNFYYAAVPSARNVPYGNIQSRLELRKKLSCKPFKWYLENVYPELRVPDHQDIAFGALQQGTNCLD
  TLGHFADGVVGVYECHNAGGNQEWALTKEKSVKHMDLCLTVVDRAPGSLIKLQGCRENDSRQKWEQIEGN
  SKLRHVGSNLCLDSRTAKSGGLSVEVCGPALSQQWKFTLNLQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291866
• ORF Size: 1599 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291866.1, NP_001278795.1
• RefSeq Size: 4562 bp
• RefSeq ORF: 1602 bp
• Locus ID: 2590
• Cytogenetics: 1q42.13
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• MW: 61.2 kDa
• Gene Summary: 'This gene encodes a member of the glycosyltransferase 2 protein family. Members of this family initiate mucin-type O-glycoslation of peptides in the Golgi apparatus. The encoded protein may be involved in O-linked glycosylation of the immunoglobulin A1 hinge region. This gene may influence triglyceride levels, and may be involved Type 2 diabetes, as well as several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]'