APPBP1 (NAE1) (NM_001286500) Human Tagged ORF Clone

CAT#: RC238788

NAE1 (myc-DDK-tagged) - Human NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NAE1
• Synonyms: A-116A10.1; APPBP1; HPP1; ula-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238788 representing NM_001286500
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAGCTGGGAAAGCTGCTCAAGGAGCAGAAGTACGACCGGCAGCTGAGGTTGTGGGGTGATCATG
  GGCAAGAGGCTTTAGAATCTGCTCATGTTTGCCTAATAAATGCAACAGCCACAGGAACTGAAATTCTTAA
  AAACTTGGTACTACCAGGTAACGTAGGTATTGGTTCGTTTACAATTATTGATGGAAATCAGGTCAGCGGA
  GAAGATGCTGGAAACAATTTCTTCCTTCAAAGAAGCAGTATCGGCAAGAACCGAGCTGAAGCTGCCATGG
  AATTCTTACAAGAATTAAATAGCGATGTCTCTGGAAGTTTTGTGGAAGAGAGTCCAGAAAACCTTCTAGA
  CAATGATCCCTCATTTTTCTGTAGGTTTACTGTTGTAGTTGCAACTCAGCTTCCTGAAAGCACTTCACTA
  CGCTTAGCAGATGTCCTCTGGAATTCCCAGATTCCTCTTTTGATCTGTAGGACATATGGACTAGTTGGTT
  ATATGAGGATCATTATAAAAGAACATCCAGTAATAGAATCTCATCCAGATAATGCATTAGAGGATCTACG
  ACTAGATAAGCCATTTCCTGAACTGAGAGAACATTTTCAGTCCTATGATTTGGATCATATGGAAAAAAAG
  GACCACAGTCATACTCCATGGATTGTGATCATAGCTAAATATTTAGCACAGTGGTATAGTGAAACAAATG
  GACGAATACCTAAAACGTATAAAGAAAAAGAGGACTTCAGAGATTTGATTAGACAAGGAATTCTAAAAAA
  TGAAAATGGGGCTCCAGAAGATGAAGAGAATTTTGAAGAAGCTATTAAAAATGTGAACACAGCACTAAAT
  ACAACTCAGATCCCAAGCAGTATTGAAGATATATTTAATGATGATCGCTGCATAAATATCACCAAACAGA
  CTCCATCATTTTGGATTTTAGCTCGTGCCTTAAAGGAATTTGTGGCCAAAGAGGGTCAAGGAAATTTACC
  TGTTCGAGGCACAATTCCTGATATGATTGCAGATTCAGGCAAATATATAAAACTGCAAAACGTTTACCGT
  GAAAAAGCAAAGAAAGATGCTGCCGCTGTGGGTAATCATGTTGCCAAATTGCTGCAGTCCATTGGCCAGG
  CACCAGAGTCCATTTCAGAGAAAGAATTAAAATTACTCTGCAGCAATTCTGCATTTCTTCGAGTGGTAAG
  ATGTCGATCCTTAGCTGAAGAATATGGTTTGGATACAATTAACAAGGATGAAATTATTTCTAGCATGGAC
  AATCCAGATAATGAAATAGTGTTGTACTTAATGTTACGGGCTGTTGATAGATTTCATAAACAACAGGGTA
  GATATCCAGGAGTATCTAACTATCAAGTTGAAGAAGATATAGGAAAGTTGAAGTCTTGTCTCACTGGCTT
  CCTTCAGGAATATGGTTTATCTGTAATGGTGAAAGATGATTATGTCCACGAATTTTGCCGATATGGAGCT
  GCTGAGCCACATACCATTGCTGCATTCTTGGGGGGAGCTGCTGCTCAAGAGGTCATCAAAATAATCACCA
  AACAATTTGTAATTTTTAATAATACTTACATTTACAGTGGCATGTCACAAACTTCAGCAACTTTCCAGTT
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238788 representing NM_001286500
  Red=Cloning site Green=Tags(s)
  
  MAQLGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGNVGIGSFTIIDGNQVSG
  EDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSL
  RLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKK
  DHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALN
  TTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYR
  EKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMD
  NPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGA
  AEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286500
• ORF Size: 1611 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001286500.1, NP_001273429.1
• RefSeq Size: 1826
• RefSeq ORF: 1614
• Locus ID: 8883
• Protein Pathways: Alzheimer's disease
• MW: 61 kDa
• Gene Summary: The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]