IL1 Receptor I (IL1R1) (NM_001288706) Human Tagged ORF Clone

CAT#: RC238794

IL1R1 (myc-DDK-tagged) - Human interleukin 1 receptor, type I (IL1R1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IL1R1
• Synonyms: CD121A; D2S1473; IL-1R-alpha; IL1R; IL1RA; P80
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238794 representing NM_001288706
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTGTTACTCAGACTTATTTGTTTCATAGCTCTACTGATTTCTTCTCTGGAGGCTGATAAATGCA
  AGGAACGTGAAGAAAAAATAATTTTAGTGTCATCTGCAAATGAAATTGATGTTCGTCCCTGTCCTCTTAA
  CCCAAATGAACACAAAGGCACTATAACTTGGTATAAAGATGACAGCAAGACACCTGTATCTACAGAACAA
  GCCTCCAGGATTCATCAACACAAAGAGAAACTTTGGTTTGTTCCTGCTAAGGTGGAGGATTCAGGACATT
  ACTATTGCGTGGTAAGAAATTCATCTTACTGCCTCAGAATTAAAATAAGTGCAAAATTTGTGGAGAATGA
  GCCTAACTTATGTTATAATGCACAAGCCATATTTAAGCAGAAACTACCCGTTGCAGGAGACGGAGGACTT
  GTGTGCCCTTATATGGAGTTTTTTAAAAATGAAAATAATGAGTTACCTAAATTACAGTGGTATAAGGATT
  GCAAACCTCTACTTCTTGACAATATACACTTTAGTGGAGTCAAAGATAGGCTCATCGTGATGAATGTGGC
  TGAAAAGCATAGAGGGAACTATACTTGTCATGCATCCTACACATACTTGGGCAAGCAATATCCTATTACC
  CGGGTAATAGAATTTATTACTCTAGAGGAAAACAAACCCACAAGGCCTGTGATTGTGAGCCCAGCTAATG
  AGACAATGGAAGTAGACTTGGGATCCCAGATACAATTGATCTGTAATGTCACCGGCCAGTTGAGTGACAT
  TGCTTACTGGAAGTGGAATGGGTCAGTAATTGATGAAGATGACCCAGTGCTAGGGGAAGACTATTACAGT
  GTGGAAAATCCTGCAAACAAAAGAAGGAGTACCCTCATCACAGTGCTTAATATATCGGAAATTGAAAGTA
  GATTTTATAAACATCCATTTACCTGTTTTGCCAAGAATACACATGGTATAGATGCAGCATATATCCAGTT
  AATATATCCAGTCACTAATTTCCAGAAGCACATGATTGGTATATGTGTCACGTTGACAGTCATAATTGTG
  TGTTCTGTTTTCATCTATAAAATCTTCAAGATTGACATTGTGCTTTGGTACAGGGATTCCTGCTATGATT
  TTCTCCCAATAAAAGTCTTGCCTGAGGTCTTGGAAAAACAGTGTGGATATAAGCTGTTCATTTATGGAAG
  GGATGACTACGTTGGGGAAGACATTGTTGAGGTCATTAATGAAAACGTAAAGAAAAGCAGAAGACTGATT
  ATCATTTTAGTCAGAGAAACATCAGGCTTCAGCTGGCTGGGTGGTTCATCTGAAGAGCAAATAGCCATGT
  ATAATGCTCTTGTTCAGGATGGAATTAAAGTTGTCCTGCTTGAGCTGGAGAAAATCCAAGACTATGAGAA
  AATGCCAGAATCGATTAAATTCATTAAGCAGAAACATGGGGCTATCCGCTGGTCAGGGGACTTTACACAG
  GGACCACAGTCTGCAAAGACAAGGTTCTGGAAGAATGTCAGGTACCACATGCCAGTCCAGCGACGGTCAC
  CTTCATCTAAACACCAGTTACTGTCACCAGCCACTAAGGAGAAACTGCAAAGAGAGGCTCACGTGCCTCT
  CGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238794 representing NM_001288706
  Red=Cloning site Green=Tags(s)
  
  MKVLLRLICFIALLISSLEADKCKEREEKIILVSSANEIDVRPCPLNPNEHKGTITWYKDDSKTPVSTEQ
  ASRIHQHKEKLWFVPAKVEDSGHYYCVVRNSSYCLRIKISAKFVENEPNLCYNAQAIFKQKLPVAGDGGL
  VCPYMEFFKNENNELPKLQWYKDCKPLLLDNIHFSGVKDRLIVMNVAEKHRGNYTCHASYTYLGKQYPIT
  RVIEFITLEENKPTRPVIVSPANETMEVDLGSQIQLICNVTGQLSDIAYWKWNGSVIDEDDPVLGEDYYS
  VENPANKRRSTLITVLNISEIESRFYKHPFTCFAKNTHGIDAAYIQLIYPVTNFQKHMIGICVTLTVIIV
  CSVFIYKIFKIDIVLWYRDSCYDFLPIKVLPEVLEKQCGYKLFIYGRDDYVGEDIVEVINENVKKSRRLI
  IILVRETSGFSWLGGSSEEQIAMYNALVQDGIKVVLLELEKIQDYEKMPESIKFIKQKHGAIRWSGDFTQ
  GPQSAKTRFWKNVRYHMPVQRRSPSSKHQLLSPATKEKLQREAHVPLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288706
• ORF Size: 1614 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001288706.1, NP_001275635.1
• RefSeq Size: 4990 bp
• RefSeq ORF: 1617 bp
• Locus ID: 3554
• Cytogenetics: 2q11.2-q12.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Apoptosis, Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, MAPK signaling pathway
• MW: 62.5 kDa
• Gene Summary: 'This gene encodes a cytokine receptor that belongs to the interleukin-1 receptor family. The encoded protein is a receptor for interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist. It is an important mediator involved in many cytokine-induced immune and inflammatory responses. This gene is located in a cluster of related cytokine receptor genes on chromosome 2q12. [provided by RefSeq, Dec 2013]'