MPP2 (NM_001278373) Human Tagged ORF Clone

CAT#: RC238807

MPP2 (myc-DDK-tagged) - Human membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2) (MPP2), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MPP2
• Synonyms: DLG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238807 representing NM_001278373
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCAAGTCCTGGACAACTTGGGATCCCTCCCCAGTGCCACGGGGGCTGCAGAGCTGGACCTGATCT
  TCCTTCGAGGCATTATGGAAAGTCCCATAGTAAGATCCCTGGCCAAGGCCCATGAGAGGCTGGAGGAGAC
  GAAGCTGGAGGCCGTGAGAGACAACAACCTGGAGCTGGTGCAGGAGATCCTGCGGGACCTGGCGCAGCTG
  GCTGAGCAGAGCAGCACAGCCGCCGAGCTGGCCCACATCCTCCAGGAGCCCCACTTCCAGTCCCTCCTGG
  AGACGCACGACTCTGTGGCCTCAAAGACCTATGAGACACCACCCCCCAGCCCTGGCCTGGACCCTACATT
  CAGCAACCAGCCTGTACCTCCCGATGCTGTGCGCATGGTGGGCATCCGCAAGACAGCCGGAGAACATCTG
  GGTGTAACGTTCCGCGTGGAGGGCGGCGAGCTGGTGATCGCGCGCATTCTGCATGGGGGCATGGTGGCTC
  AACAAGGCCTGCTGCATGTGGGTGACATCATCAAGGAGGTGAACGGGCAGCCAGTGGGCAGTGACCCCCG
  CGCACTGCAGGAGCTCCTGCGCAATGCCAGTGGCAGTGTCATCCTCAAGATCCTGCCCAGCTACCAGGAG
  CCCCATCTGCCCCGCCAGGTATTTGTGAAATGTCACTTTGACTATGACCCGGCCCGAGACAGCCTCATCC
  CCTGCAAGGAAGCAGGCCTGCGCTTCAACGCCGGGGACTTGCTCCAGATCGTAAACCAGGATGATGCCAA
  CTGGTGGCAGGCATGCCATGTCGAAGGGGGCAGTGCTGGGCTCATTCCCAGCCAGCTGCTGGAGGAGAAG
  CGGAAAGCATTTGTCAAGAGGGACCTGGAGCTGACACCAAACTCAGGGACCCTATGCGGCAGCCTTTCAG
  GAAAGAAAAAGAAGCGAATGATGTATTTGACCACCAAGAATGCAGAGTTTGACCGTCATGAGCTGCTCAT
  TTATGAGGAGGTGGCCCGCATGCCCCCGTTCCGCCGGAAAACCCTGGTACTGATTGGGGCTCAGGGCGTG
  GGACGGCGCAGCCTGAAGAACAAGCTCATCATGTGGGATCCAGATCGCTATGGCACCACGGTGCCCTACA
  CCTCCCGGCGGCCGAAAGACTCAGAGCGGGAAGGTCAGGGTTACAGCTTTGTGTCCCGTGGGGAGATGGA
  GGCTGACGTCCGTGCTGGGCGCTACCTGGAGCATGGCGAATACGAGGGCAACCTGTATGGCACACGTATT
  GACTCCATCCGGGGCGTGGTCGCTGCTGGGAAGGTGTGCGTGCTGGATGTCAACCCCCAGGCGGTGAAGG
  TGCTACGAACGGCCGAGTTTGTCCCTTACGTGGTGTTCATCGAGGCCCCAGACTTCGAGACCCTGCGGGC
  CATGAACAGGGCTGCGCTGGAGAGTGGAATATCCACCAAGCAGCTCACGGAGGCGGACCTGAGACGGACA
  GTGGAGGAGAGCAGCCGCATCCAGCGGGGCTACGGGCACTACTTTGACCTCTGCCTGGTCAATAGCAACC
  TGGAGAGGACCTTCCGCGAGCTCCAGACAGCCATGGAGAAGCTACGGACAGAGCCCCAGTGGGTGCCTGT
  CAGCTGGGTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238807 representing NM_001278373
  Red=Cloning site Green=Tags(s)
  
  MQQVLDNLGSLPSATGAAELDLIFLRGIMESPIVRSLAKAHERLEETKLEAVRDNNLELVQEILRDLAQL
  AEQSSTAAELAHILQEPHFQSLLETHDSVASKTYETPPPSPGLDPTFSNQPVPPDAVRMVGIRKTAGEHL
  GVTFRVEGGELVIARILHGGMVAQQGLLHVGDIIKEVNGQPVGSDPRALQELLRNASGSVILKILPSYQE
  PHLPRQVFVKCHFDYDPARDSLIPCKEAGLRFNAGDLLQIVNQDDANWWQACHVEGGSAGLIPSQLLEEK
  RKAFVKRDLELTPNSGTLCGSLSGKKKKRMMYLTTKNAEFDRHELLIYEEVARMPPFRRKTLVLIGAQGV
  GRRSLKNKLIMWDPDRYGTTVPYTSRRPKDSEREGQGYSFVSRGEMEADVRAGRYLEHGEYEGNLYGTRI
  DSIRGVVAAGKVCVLDVNPQAVKVLRTAEFVPYVVFIEAPDFETLRAMNRAALESGISTKQLTEADLRRT
  VEESSRIQRGYGHYFDLCLVNSNLERTFRELQTAMEKLRTEPQWVPVSWVY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278373
• ORF Size: 1623 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278373.1, NP_001265302.1
• RefSeq Size: 4581 bp
• RefSeq ORF: 1626 bp
• Locus ID: 4355
• Cytogenetics: 17q21.31
• Protein Families: Druggable Genome
• MW: 61 kDa
• Gene Summary: ' Palmitoylated membrane protein 2 is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 2 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. [provided by RefSeq, Jul 2008]'