DCP1A (NM_001290204) Human Tagged ORF Clone

CAT#: RC238825

DCP1A (myc-DDK-tagged) - Human decapping mRNA 1A (DCP1A), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DCP1A
• Synonyms: HSA275986; Nbla00360; SMAD4IP1; SMIF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238825 representing NM_001290204
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGCTGAGTCGAGCTGGGCAGGAGATGAGCCTAGCGGCCCTGAAGCAACACGACCCCTATATCA
  CCAGCATCGCAGACCTCACGGGCCAGGTCGCTCTGTACACCTTCTGCCCCAAGGCCAACCAGTGGGAGAA
  GACTGATATAGAAGGGACCTTATTCGTATATCGAAGGTCAGCTTCCCCTTACCATGGTTTTACCATTGTG
  AATCGACTAAATATGCACAATCTAGTTGAACCAGTGAATAAAGATTTGGAATTTCAGCTCCATGAACCAT
  TTCTTCTGTATAGAAATGCAAGCTTGTCGATATATAGTATCTGGTTTTATGACAAGAATGACTGTCACCG
  CATAGCAAAACTCATGGCTGATGTGGTAGAAGAGGAGACACGGCGATCCCAGCAAGCTGCTCGGGACAAA
  CAGAGTCCCAGCCAGGCCAATGGCTGCAGCGACCACAGGCCCATCGACATCCTGGAGATGCTGAGCAGAG
  CCAAGGATGAGTATGAGAGGTCTGCTCCATCTGGACACAAGCATCTGACGGTAGAAGAGTTATTTGGAAC
  CTCTTTGCCAAAGGAACAACCAGCAGTTGTGGGTCTGGATTCAGAAGAAATGGAGAGGTTGCCAGGAGAT
  GCCTCCCAGAAAGAGCCCAATTCATTCCTACCATTTCCCTTTGAGCAGTTAGGAGGAGCCCCTCAATCAG
  AAACCCTGGGTGTCCCTTCTGCTGCCCACCATTCAGTCCAGCCTGAAATCACCACCCCGGTGCTAATCAC
  TCCAGCCTCCATCACACAGTCCAATGAAAAGCATGCTCCAACCTACACAATCCCGTTGAGCCCTGTTCTC
  AGTCCCACTCTGCCAGCTGAAGCTCCTACTGCACAGGTTCCCCCCAGCTTACCTCGAAACAGCACCATGA
  TGCAGGCAGTGAAGACCACGCCTAGACAGAGGTCTCCACTCCTGAACCAGCCAGTCCCTGAGCTAAGCCA
  TGCCAGTCTGATTGCCAACCAGAGCCCCTTCAGGGCCCCATTGAACGTGACGAACACAGCTGGCACATCC
  CTCCCAAGCGTTGATCTTCTCCAGAAACTCAGGTTGACCCCACAGCATGACCAAATACAGACACAACCAC
  TTGGGAAAGGTGCAATGGTAGCCAGCTTTTCTCCGGCAGCTGGTCAGCTAGCCACACCTGAGAGCTTCAT
  AGAGCCTCCCTCTAAGACAGCAGCAGCAAGAGTGGCGGCCTCAGCCTCCCTGAGCAACATGGTGCTTGCT
  CCCCTTCAGTCTATGCAGCAGAACCAGGATCCTGAAGTATTTGTGCAGCCTAAGGTGTTATCCAGTGCCA
  TCCCGGTTGCAGGCGCCCCACTGGTTACTGCAACGACCACTGCAGTGTCTTCAGTCCTGCTGGCCCCAAG
  TGTTTTCCAGCAGACAGTTACAAGATCTTCGGACCTTGAGAGGAAAGCCAGCTCCCCTTCTCCTCTAACT
  ATTGGAACGCCAGAAAGTCAGAGAAAGCCTTCCATTATTCTCAGCAAGTCTCAGCTCCAGGATACATTAA
  TACATCTAATAAAGAATGATTCCAGCTTCCTCAGTACACTTCATGAAGTCTACTTGCAGGTTCTGACCAA
  GAACAAAGACAACCACAACCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238825 representing NM_001290204
  Red=Cloning site Green=Tags(s)
  
  MEALSRAGQEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDIEGTLFVYRRSASPYHGFTIV
  NRLNMHNLVEPVNKDLEFQLHEPFLLYRNASLSIYSIWFYDKNDCHRIAKLMADVVEEETRRSQQAARDK
  QSPSQANGCSDHRPIDILEMLSRAKDEYERSAPSGHKHLTVEELFGTSLPKEQPAVVGLDSEEMERLPGD
  ASQKEPNSFLPFPFEQLGGAPQSETLGVPSAAHHSVQPEITTPVLITPASITQSNEKHAPTYTIPLSPVL
  SPTLPAEAPTAQVPPSLPRNSTMMQAVKTTPRQRSPLLNQPVPELSHASLIANQSPFRAPLNVTNTAGTS
  LPSVDLLQKLRLTPQHDQIQTQPLGKGAMVASFSPAAGQLATPESFIEPPSKTAAARVAASASLSNMVLA
  PLQSMQQNQDPEVFVQPKVLSSAIPVAGAPLVTATTTAVSSVLLAPSVFQQTVTRSSDLERKASSPSPLT
  IGTPESQRKPSIILSKSQLQDTLIHLIKNDSSFLSTLHEVYLQVLTKNKDNHNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290204
• ORF Size: 1632 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001290204.1, NP_001277133.1
• RefSeq Size: 5913
• RefSeq ORF: 1635
• Locus ID: 55802
• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation
• MW: 59.8 kDa
• Gene Summary: Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]