GUCY1B1 (NM_001291955) Human Tagged ORF Clone

CAT#: RC238860

GUCY1B3 (myc-DDK-tagged) - Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GUCY1B1
• Synonyms: GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3; GUCY1B3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238860 representing NM_001291955
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGGGAAGATGTTTTTCGTCTTTTGCCAAGAATCTGGTTATGATACAATCTTGCGTGTCCTGGGCT
  CTAATGTCAGAGAATTTCTACAGAACCTTGATGCTCTGCACGACCACCTTGCTACCATCTACCCAGGAAT
  GCGTGCACCTTCCTTTAGGTGCACTGATGCAGAAAAGGGCAAAGGACTCATTTTGCACTACTACTCAGAG
  AGAGAAGGACTTCAGGATATTGTCATTGGAATCATCAAAACAGTGGCACAACAAATCCATGGCACTGAAA
  TAGACATGAAGGTTATTCAGCAAAGAAATGAAGAATGTGATCATACTCAATTTTTAATTGAAGAAAAAGA
  GTCAAAAGAAGAGGATTTTTATGAAGATCTTGACAGATTTGAAGAAAATGGTACCCAGGAATCACGCATC
  AGCCCATATACATTCTGCAAAGCTTTTCCTTTTCATATAATATTTGACCGGGACCTAGTGGTCACTCAGT
  GTGGCAATGCTATATACAGAGTTCTCCCCCAGCTCCAGCCTGGGAATTGCAGCCTTCTGTCTGTCTTCTC
  GCTGGTTCGTCCTCATATTGATATTAGTTTCCATGGGATCCTTTCTCACATCAATACTGTTTTTGTATTG
  AGAAGCAAGGAAGGATTGTTGGATGTGGAGAAATTAGAATGTGAGGATGAACTGACTGGGACTGAGATCA
  GCTGCTTACGTCTCAAGGGTCAAATGATCTACTTACCTGAAGCAGATAGCATACTTTTTCTATGTTCACC
  AAGTGTCATGAACCTGGACGATTTGACAAGGAGAGGGCTGTATCTAAGTGACATCCCTCTGCATGATGCC
  ACGCGCGATCTTGTTCTTTTGGGAGAACAATTTAGAGAGGAATACAAACTCACCCAAGAACTGGAAATCC
  TCACTGACAGGCTACAGCTCACGTTAAGAGCCCTGGAAGATGAAAAGAAAAAGACAGACACATTGCTGTA
  TTCTGTCCTTCCTCCGTCTGTTGCCAATGAGCTGCGGCACAAGCGTCCAGTGCCTGCCAAAAGATATGAC
  AATGTGACCATCCTCTTTAGTGGCATTGTGGGCTTCAATGCTTTCTGTAGCAAGCATGCATCTGGAGAAG
  GAGCCATGAAGATCGTCAACCTCCTCAACGACCTCTACACCAGATTTGACACACTGACTGATTCCCGGAA
  AAACCCATTTGTTTATAAGGTGGAGACTGTTGGTGACAAGTATATGACAGTGAGTGGTTTACCAGAGCCA
  TGCATTCACCATGCACGATCCATCTGCCACCTGGCCTTGGACATGATGGAAATTGCTGGCCAGGTTCAAG
  TAGATGGTGAATCTGTTCAGATAACAATAGGGATACACACTGGAGAGGTAGTTACAGGTGTCATAGGACA
  GCGGATGCCTCGATACTGTCTTTTTGGGAATACTGTCAACCTCACAAGCCGAACAGAAACCACAGGAGAA
  AAGGGAAAAATAAATGTGTCTGAATATACATACAGATGTCTTATGTCTCCAGAAAATTCAGATCCACAAT
  TCCACTTGGAGCACAGAGGCCCAGTGTCCATGAAGGGCAAAAAAGAACCAATGCAAGTTTGGTTTCTATC
  CAGAAAAAATACAGGAACAGAGGAAACAAAGCAGGATGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238860 representing NM_001291955
  Red=Cloning site Green=Tags(s)
  
  MFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSE
  REGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRI
  SPYTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVL
  RSKEGLLDVEKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDA
  TRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYD
  NVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEP
  CIHHARSICHLALDMMEIAGQVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGE
  KGKINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETKQDDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291955
• ORF Size: 1653 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291955.1, NP_001278884.1
• RefSeq Size: 3187 bp
• RefSeq ORF: 1656 bp
• Locus ID: 2983
• Cytogenetics: 4q32.1
• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction
• MW: 63.2 kDa
• Gene Summary: 'This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]'