C6orf70 (ERMARD) (NM_001278532) Human Tagged ORF Clone

CAT#: RC238870

ERMARD (myc-DDK-tagged) - Human ER membrane-associated RNA degradation (ERMARD), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ERMARD
• Synonyms: C6orf70; dJ266L20.3; PVNH6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238870 representing NM_001278532
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACTGACATCGTGTCTAGAACGAGCCTTGGGTGATGTATTTTTACTGATTGGGAAGGAATGCCCCT
  TTCTTTTAAGAGATCTGCTTTCATCTGAGGAGCTTGCTCAAGTCTTCAGTCAGTCTGTGATGAATGTGCT
  AAAAGTCTTCGTTGGCTCTCCGTGTGGTCTCAACCTGCGTAACGTCTTATGGCATGGGTTTGCGTCACCT
  GAAGAAATTCCTCCAAAATACTGTTCAATGATGATACTGTTGACGGCAGGATTGGGTCAGTTACTGAAGA
  GTTACCTTCAAAACACTAAACTTACATTGGCACATCGCTCTTTCATATCTCTTACAAACCTCGAGGATTT
  GATTGTTTTTCCTGATGTTACTTATGAGGTGCTTTCAGTATTAGAAGAAGTGATGATGAAATCTGCTTTT
  ATATTAAAAATCATGTTACCATATTGGGAAGTTGCACTGGTCAAGTTCAAGTCACACAGGTTTGCTGACT
  GCGCCATATTGTTGCTGACACAACTGGAGACTGGACTTAGGAATGTTTTTGCCACACTTAACAGATGTCC
  AAAAAGACTCCTGACTGCTGAGTCAACAGCTCTTTATACCACCTTTGATCAAATATTGGCAAAACACTTG
  AATGATGGTAAAATCAATCAGCTTCCTCTTTTCCTTGGAGAGCCTGCTATGGAATTTCTCTGGGATTTCC
  TGAACCATCAGGAGGGTCCCCGCATAAGAGATCATTTAAGCCACGGGGAGATCAACTTACATGAATTTTC
  AAAAGAAACAACTAATCAGTTGCTTGCATTTTCTCTTGTACTGCTACTCAGATTCGTTGATGACTGTCTG
  CTATCAGTTTTTAAGGAAAAATCAGCCGTAGAATTGTTGATTAGTCTTGCAGAAGGCTATAGTTCTCGCT
  GTCATCCGGTTTTTCAGCTTAAAAAACAGGTGCTGAGCTGTGAGGAGAGCATCAGGGTTTGGGCTCTGCT
  GCCTTTCCCCGAAGAACTCACTCGGCAAGCCGTCAGATTAGAAGATAATTCTGAAACAAATGCCTGCCAC
  TCTTTGATTACAAAAATGACGGATGAGCTGTATCACCATATGCCTGAGAATCGTTGTGTGTTAAAGGACT
  TGGATCGTCTTCCTACTGAGACGTGGCCCCAGCTTCTCCGTGAGCTCTGCAGCACACCTGTTCCCACCCT
  GTTCTGCCCCAGGATTGTGCTGGAAGTGCTGGTTGTGCTCCGAAGCATCAGCGAACAGTGCCGCCGTGTG
  TCCAGCCAGGTCACCGTTGCCTCAGAGCTGAGACACAGGCAGTGGGTGGAAAGGACGCTGCGGTCTCGCC
  AGCGGCAGAACTACCTGCGTATGTGGAGTAGTATCAGACTACTGTCCCCTGTGCTCAGCCTGATACTGTT
  ACTCATTGCGCTGGAGTTGGTCAACATTCATGCTGTTTGTGGGAAGAATGCGCATGAGTATCAGCAGTAC
  CTAAAGTTTGTAAAGTCGATCTTGCAGTACACGGAGAACCTGGTGGCTTACACCAGTTACGAAAAGAACA
  AGTGGAATGAAACTATCAATCTTACACATACAGCTTTGTTGAAAATGTGGACTTTTAGTGAGAAGAAACA
  AATGTTAATACATTTAGCCAAGAAATCCACAAGTAAAGTACTCTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238870 representing NM_001278532
  Red=Cloning site Green=Tags(s)
  
  MKLTSCLERALGDVFLLIGKECPFLLRDLLSSEELAQVFSQSVMNVLKVFVGSPCGLNLRNVLWHGFASP
  EEIPPKYCSMMILLTAGLGQLLKSYLQNTKLTLAHRSFISLTNLEDLIVFPDVTYEVLSVLEEVMMKSAF
  ILKIMLPYWEVALVKFKSHRFADCAILLLTQLETGLRNVFATLNRCPKRLLTAESTALYTTFDQILAKHL
  NDGKINQLPLFLGEPAMEFLWDFLNHQEGPRIRDHLSHGEINLHEFSKETTNQLLAFSLVLLLRFVDDCL
  LSVFKEKSAVELLISLAEGYSSRCHPVFQLKKQVLSCEESIRVWALLPFPEELTRQAVRLEDNSETNACH
  SLITKMTDELYHHMPENRCVLKDLDRLPTETWPQLLRELCSTPVPTLFCPRIVLEVLVVLRSISEQCRRV
  SSQVTVASELRHRQWVERTLRSRQRQNYLRMWSSIRLLSPVLSLILLLIALELVNIHAVCGKNAHEYQQY
  LKFVKSILQYTENLVAYTSYEKNKWNETINLTHTALLKMWTFSEKKQMLIHLAKKSTSKVLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278532
• ORF Size: 1656 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001278532.1, NP_001265461.1
• RefSeq Size: 1975
• RefSeq ORF: 1659
• Locus ID: 55780
• Protein Families: Transmembrane
• MW: 64 kDa
• Gene Summary: The protein encoded by this gene contains 2 transmembrane domains near the C-terminus and is localized in the endoplasmic reticulum. Knockout of this gene in developing rat brain showed that it may be involved in neuronal migration. Mutations in this gene are associated with periventricular nodular heterotopia-6 (PVNH6). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]