CRNKL1 (NM_001278626) Human Tagged ORF Clone

CAT#: RC238910

CRNKL1 (myc-DDK-tagged) - Human crooked neck pre-mRNA splicing factor 1 (CRNKL1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRNKL1
• Synonyms: CLF; Clf1; CRN; HCRN; MSTP021; SYF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238910 representing NM_001278626
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAATGAAGAATCGCCAAGTCAACCATGCTCGAAATATCTGGGACCGGGCCATAACAACGCTGCCTC
  GAGTTAATCAGTTCTGGTACAAGTACACGTACATGGAGGAAATGTTGGGAAACGTTGCCGGTGCCCGGCA
  GGTGTTTGAGCGCTGGATGGAGTGGCAGCCTGAGGAGCAAGCCTGGCACTCCTACATCAACTTTGAGCTG
  AGATACAAAGAGGTGGATCGGGCCCGCACCATTTATGAGCGATTTGTCCTCGTGCACCCTGATGTTAAGA
  ACTGGATCAAGTATGCCCGCTTTGAAGAAAAACATGCTTATTTTGCCCATGCACGGAAAGTGTATGAGAG
  AGCTGTGGAATTCTTTGGAGATGAACATATGGATGAGCACCTTTATGTTGCCTTTGCCAAGTTTGAAGAA
  AATCAGAAAGAGTTTGAAAGGGTACGAGTGATTTACAAGTATGCCCTGGACAGAATTTCAAAACAAGATG
  CCCAAGAACTCTTTAAAAATTATACCATCTTTGAGAAGAAGTTTGGTGATAGGCGGGGTATTGAAGATAT
  CATTGTGAGCAAACGGAGATTCCAGTACGAAGAAGAAGTGAAGGCGAATCCACACAATTATGATGCATGG
  TTTGATTACTTGCGCTTGGTAGAAAGTGACGCAGAAGCTGAAGCCGTGAGAGAAGTCTATGAAAGGGCCA
  TTGCCAATGTCCCACCCATTCAGGAGAAGAGGCACTGGAAGCGCTACATTTATCTTTGGATCAACTATGC
  ACTCTATGAAGAATTGGAGGCAAAGGATCCTGAGAGGACAAGACAGGTGTATCAAGCCTCTTTGGAACTA
  ATTCCTCACAAAAAGTTCACATTTGCCAAAATGTGGATACTGTATGCACAGTTTGAAATACGACAGAAGA
  ATCTGTCATTAGCCAGAAGAGCATTGGGAACTTCCATAGGCAAATGTCCAAAGAACAAATTATTTAAAGT
  TTACATAGAATTGGAGCTACAGCTTCGAGAATTTGACAGATGCCGGAAGCTTTATGAAAAGTTCCTGGAA
  TTTGGACCTGAAAATTGTACCTCATGGATTAAATTCGCTGAATTAGAGACAATCCTTGGTGATATTGACA
  GAGCACGGGCAATCTATGAATTAGCCATCAGTCAGCCACGTTTAGACATGCCAGAGGTGCTTTGGAAATC
  ATATATTGATTTTGAAATTGAGCAGGAAGAAACAGAAAGAACACGAAACCTTTACCGGCGGTTGCTTCAA
  CGGACGCAGCATGTCAAGGTATGGATCAGCTTTGCTCAGTTTGAGTTGTCTTCAGGAAAAGAAGGAAGTT
  TGACTAAATGCAGACAAATTTATGAAGAAGCTAACAAAACCATGCGAAACTGTGAAGAAAAGGAAGAGAG
  ACTTATGCTGCTGGAATCTTGGCGAAGTTTTGAAGAAGAATTTGGAACAGCTTCAGATAAGGAGAGAGTA
  GACAAACTCATGCCAGAGAAAGTCAAGAAGAGAAGAAAGGTCCAGACTGATGATGGGTCTGATGCAGGCT
  GGGAAGAATACTTTGATTACATCTTTCCAGAAGATGCTGCCAACCAACCTAACCTCAAACTCCTGGCCAT
  GGCCAAACTGTGGAAGAAACAGCAGCAGGAAAAGGAGGATGCTGAGCACCATCCAGATGAGGACGTCGAT
  GAGAGTGAATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238910 representing NM_001278626
  Red=Cloning site Green=Tags(s)
  
  MEMKNRQVNHARNIWDRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFEL
  RYKEVDRARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFAKFEE
  NQKEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQYEEEVKANPHNYDAW
  FDYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINYALYEELEAKDPERTRQVYQASLEL
  IPHKKFTFAKMWILYAQFEIRQKNLSLARRALGTSIGKCPKNKLFKVYIELELQLREFDRCRKLYEKFLE
  FGPENCTSWIKFAELETILGDIDRARAIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQ
  RTQHVKVWISFAQFELSSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERV
  DKLMPEKVKKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPDEDVD
  ESES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278626
• ORF Size: 1692 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001278626.1, NP_001265555.1
• RefSeq Size: 4190
• RefSeq ORF: 1695
• Locus ID: 51340
• Protein Pathways: Spliceosome
• MW: 69.1 kDa
• Gene Summary: The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013]