CRMP1 (NM_001288662) Human Tagged ORF Clone

CAT#: RC238917

CRMP1 (myc-DDK-tagged) - Human collapsin response mediator protein 1 (CRMP1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRMP1
• Synonyms: CRMP-1; DPYSL1; DRP-1; DRP1; ULIP-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238917 representing NM_001288662
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGACCACTGGAGCCCATAGTGACCGACTCCTCATCAAAGGTGGACGGATCATCAACGATGACCAAT
  CCCTTTATGCTGACGTCTACCTGGAGGATGGACTTATCAAACAAATAGGAGAGAACTTAATCGTTCCTGG
  TGGAGTGAAGACCATTGAAGCCAACGGGCGGATGGTTATTCCCGGAGGTATTGATGTCAACACGTACCTG
  CAGAAGCCCTCCCAGGGGATGACTGCGGCTGATGACTTCTTCCAAGGGACCAGGGCGGCACTGGTGGGCG
  GGACCACGATGATCATTGACCATGTTGTTCCTGAACCTGGGTCCAGCCTACTGACCTCTTTCGAGAAGTG
  GCACGAAGCAGCTGACACCAAATCCTGCTGTGATTACTCCCTCCACGTGGACATCACAAGCTGGTACGAT
  GGCGTTCGGGAGGAGCTGGAGGTGCTGGTGCAGGACAAAGGCGTCAATTCCTTCCAAGTCTACATGGCCT
  ATAAGGATGTCTACCAAATGTCCGACAGCCAGCTCTATGAAGCCTTTACCTTCCTTAAGGGCCTGGGAGC
  TGTGATCTTGGTCCATGCAGAAAATGGAGATTTGATAGCTCAGGAACAAAAGCGGATCCTGGAGATGGGC
  ATCACGGGTCCCGAGGGCCATGCCCTGAGCAGACCTGAAGAGCTGGAGGCCGAGGCGGTGTTCCGGGCCA
  TCACCATTGCGGGCCGGATCAACTGCCCTGTGTACATCACCAAGGTCATGAGCAAGAGTGCAGCCGACAT
  CATCGCTCTGGCCAGGAAGAAAGGGCCCCTAGTTTTTGGAGAGCCCATTGCCGCCAGCCTGGGGACCGAT
  GGCACCCATTACTGGAGCAAGAACTGGGCCAAGGCTGCGGCGTTCGTGACTTCCCCTCCCCTGAGCCCGG
  ACCCTACCACGCCCGACTACTTGACCTCCCTACTGGCCTGTGGGGACTTGCAGGTCACAGGCAGCGGCCA
  CTGTCCCTACAGCACTGCCCAGAAGGCGGTGGGCAAGGACAACTTTACCCTGATCCCCGAGGGTGTCAAC
  GGGATAGAGGAGCGGATGACGGTCGTCTGGGACAAGGCGGTGGCTACTGGCAAAATGGATGAGAACCAGT
  TTGTCGCTGTCACCAGCACCAATGCAGCCAAGATCTTTAACCTGTACCCAAGGAAAGGGCGGATTGCCGT
  GGGCTCGGATGCCGACGTGGTCATCTGGGACCCCGACAAGTTGAAGACCATAACAGCCAAAAGTCACAAG
  TCGGCGGTGGAGTACAACATCTTCGAGGGTATGGAGTGCCACGGCTCCCCACTAGTGGTCATCAGCCAGG
  GCAAGATCGTCTTTGAAGACGGAAACATCAACGTCAACAAGGGCATGGGCCGCTTCATTCCGCGGAAGGC
  GTTCCCGGAGCACCTGTACCAGCGCGTCAAAATCAGGAATAAGGTTTTTGGATTGCAAGGGGTTTCCAGG
  GGCATGTATGACGGTCCTGTGTACGAGGTACCAGCTACACCCAAATATGCAACTCCCGCTCCTTCAGCCA
  AATCTTCGCCTTCTAAACACCAGCCCCCACCCATCAGAAACCTCCACCAGTCCAACTTCAGCTTATCAGG
  TGCCCAGATAGATGACAACAATCCCAGGCGCACCGGCCACCGCATCGTGGCGCCCCCTGGTGGCCGCTCC
  AACATCACCAGCCTCGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238917 representing NM_001288662
  Red=Cloning site Green=Tags(s)
  
  MRTTGAHSDRLLIKGGRIINDDQSLYADVYLEDGLIKQIGENLIVPGGVKTIEANGRMVIPGGIDVNTYL
  QKPSQGMTAADDFFQGTRAALVGGTTMIIDHVVPEPGSSLLTSFEKWHEAADTKSCCDYSLHVDITSWYD
  GVREELEVLVQDKGVNSFQVYMAYKDVYQMSDSQLYEAFTFLKGLGAVILVHAENGDLIAQEQKRILEMG
  ITGPEGHALSRPEELEAEAVFRAITIAGRINCPVYITKVMSKSAADIIALARKKGPLVFGEPIAASLGTD
  GTHYWSKNWAKAAAFVTSPPLSPDPTTPDYLTSLLACGDLQVTGSGHCPYSTAQKAVGKDNFTLIPEGVN
  GIEERMTVVWDKAVATGKMDENQFVAVTSTNAAKIFNLYPRKGRIAVGSDADVVIWDPDKLKTITAKSHK
  SAVEYNIFEGMECHGSPLVVISQGKIVFEDGNINVNKGMGRFIPRKAFPEHLYQRVKIRNKVFGLQGVSR
  GMYDGPVYEVPATPKYATPAPSAKSSPSKHQPPPIRNLHQSNFSLSGAQIDDNNPRRTGHRIVAPPGGRS
  NITSLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288662
• ORF Size: 1698 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001288662.1, NP_001275591.1
• RefSeq Size: 3101 bp
• RefSeq ORF: 1701 bp
• Locus ID: 1400
• Cytogenetics: 4p16.2
• MW: 61.9 kDa
• Gene Summary: 'This gene encodes a member of a family of cytosolic phosphoproteins expressed exclusively in the nervous system. The encoded protein is thought to be a part of the semaphorin signal transduction pathway implicated in semaphorin-induced growth cone collapse during neural development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]'