GUCY1B1 (NM_001291952) Human Tagged ORF Clone

CAT#: RC239043

GUCY1B3 (myc-DDK-tagged) - Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GUCY1B1
• Synonyms: GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3; GUCY1B3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239043 representing NM_001291952
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTATGTGCTTTATAAAAGAGGCACAGTTAGATGAAGAAGGACAGTTTCTTGTCAGAATAATATATG
  ATGACTCCAAAACTTATGATTTGGTTGCTGCTGCAAGCAAAGTCCTCAATCTCAATGCTGGAGAAATCCT
  CCAAATGTTTGGGAAGATGTTTTTCGTCTTTTGCCAAGAATCTGGTTATGATACAATCTTGCGTGTCCTG
  GGCTCTAATGTCAGAGAATTTCTACAGAACCTTGATGCTCTGCACGACCACCTTGCTACCATCTACCCAG
  GAATGCGTGCACCTTCCTTTAGGTGCACTGATGCAGAAAAGGGCAAAGGACTCATTTTGCACTACTACTC
  AGAGAGAGAAGGACTTCAGGATATTGTCATTGGAATCATCAAAACAGTGGCACAACAAATCCATGGCACT
  GAAATAGACATGAAGGTTATTCAGCAAAGAAATGAAGAATGTGATCATACTCAATTTTTAATTGAAGAAA
  AAGAGTCAAAAGAAGAGGATTTTTATGAAGATCTTGACAGATTTGAAGAAAATGGTACCCAGGAATCACG
  CATCAGCCCATATACATTCTGCAAAGCTTTTCCTTTTCATATAATATTTGACCGGGACCTAGTGGTCACT
  CAGTGTGGCAATGCTATATACAGAGTTCTCCCCCAGCTCCAGCCTGGGAATTGCAGCCTTCTGTCTGTCT
  TCTCGCTGGTTCGTCCTCATATTGATATTAGTTTCCATGGGATCCTTTCTCACATCAATACTGTTTTTGT
  ATTGAGAAGCAAGGAAGGATTGTTGGATGTGGAGAAATTAGAATGTGAGGATGAACTGACTGGGACTGAG
  ATCAGCTGCTTACGTCTCAAGGGTCAAATGATCTACTTACCTGAAGCAGATAGCATACTTTTTCTATGTT
  CACCAAGTGTCATGAACCTGGACGATTTGACAAGGAGAGGGCTGTATCTAAGTGACATCCCTCTGCATGA
  TGCCACGCGCGATCTTGTTCTTTTGGGAGAACAATTTAGAGAGGAATACAAACTCACCCAAGAACTGGAA
  ATCCTCACTGACAGGCTACAGCTCACGTTAAGAGCCCTGGAAGATGAAAAGAAAAAGACAGACACATTGC
  TGTATTCTGTCCTTCCTCCGTCTGTTGCCAATGAGCTGCGGCACAAGCGTCCAGTGCCTGCCAAAAGATA
  TGACAATGTGACCATCCTCTTTAGTGGCATTGTGGGCTTCAATGCTTTCTGTAGCAAGCATGCATCTGGA
  GAAGGAGCCATGAAGATCGTCAACCTCCTCAACGACCTCTACACCAGATTTGACACACTGACTGATTCCC
  GGAAAAACCCATTTGTTTATAAGGTGGAGACTGTTGGTGACAAGTATATGACAGTGAGTGGTTTACCAGA
  GCCATGCATTCACCATGCACGATCCATCTGCCACCTGGCCTTGGACATGATGGAAATTGCTGGCCAGGTT
  CAAGTAGATGGTGAATCTGTTCAGATAACAATAGGGATACACACTGGAGAGGTAGTTACAGGTGTCATAG
  GACAGCGGATGCCTCGATACTGTCTTTTTGGGAATACTGTCAACCTCACAAGCCGAACAGAAACCACAGG
  AGAAAAGGGAAAAATAAATGTGTCTGAATATACATACAGATGTCTTATGTCTCCAGAAAATTCAGATCCA
  CAATTCCACTTGGAGCACAGAGGCCCAGTGTCCATGAAGGGCAAAAAAGAACCAATGCAAGTTTGGTTTC
  TATCCAGAAAAAATACAGGAACAGAGGAAACAAAGCAGGATGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239043 representing NM_001291952
  Red=Cloning site Green=Tags(s)
  
  MLMCFIKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVL
  GSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGT
  EIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPYTFCKAFPFHIIFDRDLVVT
  QCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKLECEDELTGTE
  ISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELE
  ILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASG
  EGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQV
  QVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDP
  QFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETKQDDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291952
• ORF Size: 1797 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291952.1, NP_001278881.1
• RefSeq Size: 3413 bp
• RefSeq ORF: 1800 bp
• Locus ID: 2983
• Cytogenetics: 4q32.1
• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction
• MW: 68.6 kDa
• Gene Summary: 'This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]'