AXL (NM_001278599) Human Tagged ORF Clone

CAT#: RC239138

AXL (myc-DDK-tagged) - Human AXL receptor tyrosine kinase (AXL), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AXL
• Synonyms: ARK; JTK11; Tyro7; UFO
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239138 representing NM_001278599
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATCCAGGCGGGAGAACCAGACCCCCCAGAGGAGCCCCTCACCTCGCAAGCATCCGTGCCCCCCC
  ATCAGCTTCGGCTAGGCAGCCTCCATCCTCACACCCCTTATCACATCCGCGTGGCATGCACCAGCAGCCA
  GGGCCCCTCATCCTGGACCCACTGGCTTCCTGTGGAGACGCCGGAGGGAGTGCCCCTGGGCCCCCCTGAG
  AACATTAGTGCTACGCGGAATGGGAGCCAGGCCTTCGTGCATTGGCAAGAGCCCCGGGCGCCCCTGCAGG
  GTACCCTGTTAGGGTACCGGCTGGCGTATCAAGGCCAGGACACCCCAGAGGTGCTAATGGACATAGGGCT
  AAGGCAAGAGGTGACCCTGGAGCTGCAGGGGGACGGGTCTGTGTCCAATCTGACAGTGTGTGTGGCAGCC
  TACACTGCTGCTGGGGATGGACCCTGGAGCCTCCCAGTACCCCTGGAGGCCTGGCGCCCAGGGCAAGCAC
  AGCCAGTCCACCAGCTGGTGAAGGAACCTTCAACTCCTGCCTTCTCGTGGCCCTGGTGGTATGTACTGCT
  AGGAGCAGTCGTGGCCGCTGCCTGTGTCCTCATCTTGGCTCTCTTCCTTGTCCACCGGCGAAAGAAGGAG
  ACCCGTTATGGAGAAGTGTTTGAACCAACAGTGGAAAGAGGTGAACTGGTAGTCAGGTACCGCGTGCGCA
  AGTCCTACAGTCGTCGGACCACTGAAGCTACCTTGAACAGCCTGGGCATCAGTGAAGAGCTGAAGGAGAA
  GCTGCGGGATGTGATGGTGGACCGGCACAAGGTGGCCCTGGGGAAGACTCTGGGAGAGGGAGAGTTTGGA
  GCTGTGATGGAAGGCCAGCTCAACCAGGACGACTCCATCCTCAAGGTGGCTGTGAAGACGATGAAGATTG
  CCATCTGCACGAGGTCAGAGCTGGAGGATTTCCTGAGTGAAGCGGTCTGCATGAAGGAATTTGACCATCC
  CAACGTCATGAGGCTCATCGGTGTCTGTTTCCAGGGTTCTGAACGAGAGAGCTTCCCAGCACCTGTGGTC
  ATCTTACCTTTCATGAAACATGGAGACCTACACAGCTTCCTCCTCTATTCCCGGCTCGGGGACCAGCCAG
  TGTACCTGCCCACTCAGATGCTAGTGAAGTTCATGGCAGACATCGCCAGTGGCATGGAGTATCTGAGTAC
  CAAGAGATTCATACACCGGGACCTGGCGGCCAGGAACTGCATGCTGAATGAGAACATGTCCGTGTGTGTG
  GCGGACTTCGGGCTCTCCAAGAAGATCTACAATGGGGACTACTACCGCCAGGGACGTATCGCCAAGATGC
  CAGTCAAGTGGATTGCCATTGAGAGTCTAGCTGACCGTGTCTACACCAGCAAGAGCGATGTGTGGTCCTT
  CGGGGTGACAATGTGGGAGATTGCCACAAGAGGCCAAACCCCATATCCGGGCGTGGAGAACAGCGAGATT
  TATGACTATCTGCGCCAGGGAAATCGCCTGAAGCAGCCTGCGGACTGTCTGGATGGACTGTATGCCTTGA
  TGTCGCGGTGCTGGGAGCTAAATCCCCAGGACCGGCCAAGTTTTACAGAGCTGCGGGAAGATTTGGAGAA
  CACACTGAAGGCCTTGCCTCCTGCCCAGGAGCCTGACGAAATCCTCTATGTCAACATGGATGAGGGTGGA
  GGTTATCCTGAACCCCCTGGAGCTGCAGGAGGAGCTGACCCCCCAACCCAGCCAGACCCTAAGGATTCCT
  GTAGCTGCCTCACTGCGGCTGAGGTCCATCCTGCTGGACGCTATGTCCTCTGCCCTTCCACAACCCCTAG
  CCCCGCTCAGCCTGCTGATAGGGGCTCCCCAGCAGCCCCAGGGCAGGAGGATGGTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239138 representing NM_001278599
  Red=Cloning site Green=Tags(s)
  
  MGIQAGEPDPPEEPLTSQASVPPHQLRLGSLHPHTPYHIRVACTSSQGPSSWTHWLPVETPEGVPLGPPE
  NISATRNGSQAFVHWQEPRAPLQGTLLGYRLAYQGQDTPEVLMDIGLRQEVTLELQGDGSVSNLTVCVAA
  YTAAGDGPWSLPVPLEAWRPGQAQPVHQLVKEPSTPAFSWPWWYVLLGAVVAAACVLILALFLVHRRKKE
  TRYGEVFEPTVERGELVVRYRVRKSYSRRTTEATLNSLGISEELKEKLRDVMVDRHKVALGKTLGEGEFG
  AVMEGQLNQDDSILKVAVKTMKIAICTRSELEDFLSEAVCMKEFDHPNVMRLIGVCFQGSERESFPAPVV
  ILPFMKHGDLHSFLLYSRLGDQPVYLPTQMLVKFMADIASGMEYLSTKRFIHRDLAARNCMLNENMSVCV
  ADFGLSKKIYNGDYYRQGRIAKMPVKWIAIESLADRVYTSKSDVWSFGVTMWEIATRGQTPYPGVENSEI
  YDYLRQGNRLKQPADCLDGLYALMSRCWELNPQDRPSFTELREDLENTLKALPPAQEPDEILYVNMDEGG
  GYPEPPGAAGGADPPTQPDPKDSCSCLTAAEVHPAGRYVLCPSTTPSPAQPADRGSPAAPGQEDGA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278599
• ORF Size: 1878 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278599.1, NP_001265528.1
• RefSeq Size: 4128 bp
• RefSeq ORF: 1881 bp
• Locus ID: 558
• Cytogenetics: 19q13.2
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• MW: 69.7 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]'