PIF1 (NM_001286496) Human Tagged ORF Clone

CAT#: RC239183

PIF1 (myc-DDK-tagged) - Human PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PIF1
• Synonyms: C15orf20; PIF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239183 representing NM_001286496
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTCGGGCATAGAGGCGGCGGCAGGGGAATATGAGGACTCGGAGCTGCGGTGCCGCGTGGCTGTGG
  AGGAGCTGAGCCCGGGCGGGCAGCCGCGAAGGCGCCAGGCCCTGCGCACCGCGGAGCTGAGCCTGGGTCG
  CAACGAGCGCCGCGAGTTGATGCTGCGGCTGCAAGCGCCAGGGCCCGCGGGGCGGCCGCGCTGCTTCCCT
  CTGCGCGCCGCGCGCCTCTTCACGCGTTTCGCCGAGGCCGGGCGCAGCACCCTGCGGCTCCCCGCCCACG
  ACACCCCCGGGGCCGGCGCAGTGCAGCTGCTGCTCTCGGACTGCCCCCCAGACCGCCTGCGCCGCTTCCT
  GCGCACATTGCGCCTCAAGCTGGCTGCGGCCCCGGGTCCCGGGCCGGCCTCCGCCCGAGCGCAGCTGCTG
  GGCCCGCGGCCCCGCGACTTCGTCACCATCAGCCCTGTGCAGCCCGAGGAGCGGCGGCTCAGGGCGGCCA
  CCCGGGTTCCGGACACTACGCTGGTGAAGCGGCCTGTGGAGCCCCAGGCTGGGGCCGAGCCTAGCACAGA
  AGCCCCAAGGTGGCCCCTGCCTGTGAAGAGGCTGAGCTTGCCCTCCACCAAGCCACAGCTTTCTGAGGAA
  CAGGCTGCTGTGCTGAGGGCCGTCCTGAAAGGCCAGAGCATCTTCTTCACTGGGAGTGCAGGAACAGGGA
  AGTCATATCTGCTAAAGCGAATCCTGGGCTCACTGCCCCCCACAGGCACTGTGGCCACTGCCAGCACTGG
  GGTGGCAGCCTGCCACATCGGGGGCACCACCCTCCATGCCTTTGCAGGCATCGGCTCAGGCCAGGCTCCT
  CTAGCCCAGTGTGTGGCCCTGGCCCAAAGGCCAGGCGTGCGGCAGGGCTGGCTGAACTGCCAGCGGTTGG
  TCATTGACGAGATCTCAATGGTGGAGGCAGACCTGTTTGACAAACTGGAGGCCGTGGCCAGAGCTGTCCG
  GCAGCAGAACAAGCCATTCGGAGGGATCCAGCTCATCATCTGTGGGGACTTTCTGCAGCTGCCACCTGTG
  ACCAAGGGCTCCCAGCCCCCACGGTTCTGCTTCCAGTCCAAGAGCTGGAAGAGGTGTGTGCCAGTGACCC
  TGGAGCTGACCAAGGTGTGGAGGCAGGCAGACCAGACCTTCATCTCTCTACTGCAGGCCGTGAGGCTAGG
  CAGGTGTTCAGATGAGGTGACCCGCCAGCTCCAGGCCACAGCTTCCCACAAGGTGGGGCGAGATGGGATT
  GTGGCCACGAGGCTCTGCACCCACCAGGATGATGTGGCCCTCACCAACGAGAGGCGGCTTCAGGAGCTGC
  CAGGTAAGGTACACAGATTTGAGGCTATGGACAGCAACCCTGAGCTGGCCAGTACCCTGGATGCCCAGTG
  TCCTGTTAGCCAGCTCCTTCAACTAAAGCTGGGGGCCCAGGTGATGCTGGTGAAAAACTTATCGGTGTCT
  CGGGGCCTGGTGAATGGTGCCCGAGGGGTGGTAGTTGGGTTCGAGGCAGAAGGGAGAGGGCTACCCCAGG
  TGCGGTTCCTGTGTGGAGTCACTGAGGTCATCCACGCTGACCGCTGGACGGTGCAGGCCACCGGGGGCCA
  GCTCCTCAGTCGGCAGCAGCTGCCCCTCCAGCTGGCCTGGGCGATGTCCATCCACAAGAGCCAAGGCATG
  ACCCTGGATTGTGTGGAGATTTCTCTGGGCCGTGTGTTTGCCAGTGGCCAGGCCTATGTGGCCCTTTCTC
  GGGCCCGCAGCCTGCAGGGCCTACGTGTGCTGGACTTTGACCCCATGGCGGTTCGCTGTGACCCCCGTGT
  GCTGCACTTCTATGCCACCCTGCGGCGGGGCAGGAGCCTCAGTCTGGAGTCCCCAGATGATGATGAGGCA
  GCCTCAGACCAGGAGAACATGGACCCAATCCTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239183 representing NM_001286496
  Red=Cloning site Green=Tags(s)
  
  MLSGIEAAAGEYEDSELRCRVAVEELSPGGQPRRRQALRTAELSLGRNERRELMLRLQAPGPAGRPRCFP
  LRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPPDRLRRFLRTLRLKLAAAPGPGPASARAQLL
  GPRPRDFVTISPVQPEERRLRAATRVPDTTLVKRPVEPQAGAEPSTEAPRWPLPVKRLSLPSTKPQLSEE
  QAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAP
  LAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPV
  TKGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGI
  VATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVS
  RGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQLLSRQQLPLQLAWAMSIHKSQGM
  TLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSLESPDDDEA
  ASDQENMDPIL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286496
• ORF Size: 1923 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001286496.1, NP_001273425.1
• RefSeq Size: 2718
• RefSeq ORF: 1926
• Locus ID: 80119
• MW: 69.8 kDa
• Gene Summary: This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus. [provided by RefSeq, Nov 2013]