CUL4A (NM_001278513) Human Tagged ORF Clone

CAT#: RC239240

CUL4A (myc-DDK-tagged) - Human cullin 4A (CUL4A), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CUL4A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239240 representing NM_001278513
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTACAAGCAACTGCGTCAGGCCTGTGAAGACCACGTCCAGGCACAGATCCTTCCGTTTAGAGAAG
  ACTCACTAGATAGTGTTTTATTTTTAAAGAAGATTAACACGTGCTGGCAGGACCACTGCAGACAAATGAT
  CATGATCAGAAGCATCTTCCTGTTCTTGGACCGCACCTATGTGCTGCAGAACTCCACGCTGCCCTCCATC
  TGGGATATGGGATTAGAACTGTTTAGAACCCATATTATTAGTGATAAAATGGTTCAGAGTAAAACCATTG
  ATGGAATCCTACTGCTGATCGAGCGCGAGAGGAGCGGCGAGGCCGTGGACCGGAGCCTGTTGCGGAGCCT
  CCTGGGCATGCTGTCTGACCTGCAGGTGTATAAAGATTCATTTGAACTGAAATTTTTGGAAGAGACTAAT
  TGCTTATATGCTGCCGAAGGCCAAAGGTTAATGCAGGAAAGAGAGGTTCCAGAATATCTTAACCATGTAA
  GTAAACGCTTAGAGGAAGAGGGAGACAGAGTAATCACTTACTTGGACCACAGCACACAGAAACCACTGAT
  TGCTTGTGTGGAGAAACAGCTATTAGGAGAACATTTAACAGCAATTCTGCAGAAAGGGCTCGACCACTTA
  CTGGATGAGAACAGAGTGCCGGACCTCGCACAGATGTACCAGCTGTTCAGCCGGGTGAGGGGCGGGCAGC
  AGGCGCTGCTGCAGCACTGGAGCGAGTACATCAAGACTTTTGGAACAGCGATCGTAATCAATCCTGAGAA
  AGACAAAGACATGGTCCAAGACCTGTTGGACTTCAAGGACAAGGTGGACCACGTGATCGAGGTCTGCTTC
  CAGAAGAATGAGCGGTTCGTCAACCTGATGAAGGAGTCCTTTGAGACGTTCATCAACAAGAGACCCAACA
  AGCCTGCAGAACTGATCGCAAAGCATGTGGATTCAAAGTTAAGAGCAGGCAACAAAGAAGCCACAGACGA
  GGAGCTGGAGCGGACGTTGGACAAGATCATGATCCTGTTCAGGTTTATCCACGGTAAAGATGTCTTTGAA
  GCATTTTATAAAAAAGATTTGGCAAAAAGACTCCTTGTTGGGAAAAGTGCCTCAGTCGATGCTGAAAAGT
  CTATGTTGTCAAAGCTCAAGCATGAGTGCGGTGCAGCCTTCACCAGCAAGCTGGAAGGCATGTTCAAGGA
  CATGGAGCTTTCGAAGGACATCATGGTTCATTTCAAGCAGCATATGCAGAATCAGAGTGACTCAGGCCCT
  ATAGACCTCACAGTGAACATACTCACAATGGGCTACTGGCCAACATACACGCCCATGGAAGTGCACTTAA
  CCCCAGAAATGATTAAACTTCAGGAAGTATTTAAGGCATTTTATCTTGGAAAGCACAGTGGTCGAAAACT
  TCAGTGGCAAACTACTTTGGGACATGCTGTTTTAAAAGCGGAGTTTAAAGAAGGGAAGAAGGAATTCCAG
  GTGTCCCTCTTCCAGACACTGGTGCTCCTCATGTTCAACGAGGGAGATGGCTTCAGCTTTGAGGAGATAA
  AAATGGCCACGGGGATAGAGGATAGTGAATTGCGCAGAACGCTGCAGTCCCTGGCCTGTGGCAAAGCACG
  TGTGCTGATTAAAAGTCCCAAAGGAAAGGAAGTGGAAGATGGAGACAAGTTCATTTTTAATGGAGAGTTC
  AAGCACAAGTTGTTTAGAATAAAGATCAATCAAATTCAGATGAAGGAAACTGTTGAGGAACAGGTTAGCA
  CCACTGAGAGAGTGTTTCAGGATAGACAATATCAGATTGATGCTGCTATCGTCAGAATAATGAAGATGAG
  AAAGACTCTTGGTCATAATCTTCTAGTTTCTGAATTATATAATCAGCTGAAATTTCCAGTAAAGCCTGGA
  GATTTGAAAAAGAGAATTGAATCTCTGATAGACAGAGACTATATGGAGAGAGACAAAGACAATCCGAATC
  AGTACCACTACGTGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239240 representing NM_001278513
  Red=Cloning site Green=Tags(s)
  
  MLYKQLRQACEDHVQAQILPFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTYVLQNSTLPSI
  WDMGLELFRTHIISDKMVQSKTIDGILLLIERERSGEAVDRSLLRSLLGMLSDLQVYKDSFELKFLEETN
  CLYAAEGQRLMQEREVPEYLNHVSKRLEEEGDRVITYLDHSTQKPLIACVEKQLLGEHLTAILQKGLDHL
  LDENRVPDLAQMYQLFSRVRGGQQALLQHWSEYIKTFGTAIVINPEKDKDMVQDLLDFKDKVDHVIEVCF
  QKNERFVNLMKESFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERTLDKIMILFRFIHGKDVFE
  AFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQSDSGP
  IDLTVNILTMGYWPTYTPMEVHLTPEMIKLQEVFKAFYLGKHSGRKLQWQTTLGHAVLKAEFKEGKKEFQ
  VSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNGEF
  KHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPG
  DLKKRIESLIDRDYMERDKDNPNQYHYVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278513
• ORF Size: 1977 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001278513.1, NP_001265442.1
• RefSeq Size: 4053
• RefSeq ORF: 1980
• Locus ID: 8451
• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis
• MW: 77.3 kDa
• Gene Summary: CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins (Liu et al., 2009 [PubMed 19481525]). [supplied by OMIM, Oct 2009]