ZNF133 (NM_001283007) Human Tagged ORF Clone

CAT#: RC239266

ZNF133 (myc-DDK-tagged) - Human zinc finger protein 133 (ZNF133), transcript variant 15

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF133
• Synonyms: pHZ-13; pHZ-66; ZNF150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239266 representing NM_001283007
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGCTGCCGGCCTTCAGGGCGCGCCTGGTGGCGCCGAGCATCCTGGGAGGTGTGGTCCGGGCGCTG
  TGCCGAGGATTCGGGGTAGTGTAGTCCTGGCGCCCCGCTGGAGGAGCTCCCCAGCTGGTGGCTGGAGCGA
  CCCCTTGTTCTTTGGTGGCGCTGGAATTTCATTTTCTAAACCAGAACTCATCACCCAGCTGGAGCAAGGG
  AAAGAGACCTGGAGAGAGGAAAAAAAATGTTCACCGGCAACCTGTCCAGATCCAGAGCCAGAGCTCTACC
  TCGATCCTTTCTGCCCTCCGGGTTTCTCCAGTCAGAAATTCCCCATGCAGCATGTGCTGTGTAATCATCC
  CCCCTGGATCTTCACATGCTTGTGTGCAGAAGGTAACATCCAGCCTGGGGATCCGGGCCCAGGGGACCAG
  GAGAAGCAGCAACAAGCCTCTGAGGGGAGACCCTGGAGTGATCAAGCAGAAGGTCCTGAGGGAGAAGGTG
  CCATGCCTTTGTTTGGAAGAACCAAGAAAAGGACTCTGGGAGCGTTCTCCAGGCCACCCCAGAGGCAGCC
  AGTCAGCTCTCGGAACGGCCTCAGAGGGGTGGAGTTAGAAGCCAGCCCAGCTCAGACAGGGAACCCTGAG
  GAAACAGACAAATTGTTGAAGAGGATAGAAGTCTTAGGATTTGGAACAGTCAACTGTGGAGAGTGTGGAC
  TGAGCTTCAGCAAGATGACAAACCTGCTCAGTCACCAGCGGATACACTCAGGGGAGAAGCCCTACGTGTG
  TGGGGTATGTGAGAAGGGCTTCAGCCTAAAGAAGAGCCTCGCCAGACACCAGAAGGCACACTCGGGGGAG
  AAGCCAATTGTGTGCAGGGAGTGTGGACGAGGCTTTAACCGGAAGTCAACGCTAATCATACACGAACGGA
  CACACTCCGGTGAGAAACCTTACATGTGCAGTGAGTGTGGGCGAGGCTTCAGCCAGAAGTCAAACCTCAT
  CATACACCAGAGGACACACTCAGGGGAAAAGCCTTACGTGTGCCGGGAATGTGGCAAAGGCTTCAGCCAG
  AAGTCAGCTGTCGTGAGACACCAGAGGACACACTTGGAGGAGAAGACCATCGTGTGCAGTGACTGTGGCC
  TGGGCTTCAGCGACAGGTCAAACCTCATCTCCCACCAGAGGACGCACTCTGGGGAGAAGCCCTACGCCTG
  CAAGGAGTGTGGGCGATGCTTCAGGCAGAGGACCACCCTTGTCAACCACCAGAGGACACACTCAAAGGAG
  AAGCCCTATGTGTGCGGGGTGTGTGGGCACAGCTTCAGCCAGAATTCAACCCTCATCTCTCACAGGCGGA
  CACACACTGGGGAGAAGCCGTATGTTTGTGGGGTGTGTGGGCGAGGCTTTAGTCTCAAGTCACACCTCAA
  CAGACACCAGAACATACACTCAGGAGAGAAGCCCATTGTGTGCAAGGACTGTGGCCGGGGCTTCAGCCAG
  CAATCCAACCTCATCAGACACCAGAGGACGCACTCAGGCGAGAAGCCCATGGTGTGTGGGGAGTGCGGGC
  GAGGCTTCAGCCAGAAGTCAAACCTTGTTGCACACCAGAGGACGCACTCAGGGGAGAGGCCGTATGTGTG
  CCGAGAGTGCGGGCGAGGCTTTAGCCACCAGGCCGGTCTCATCAGGCACAAGCGGAAGCACTCGAGGGAG
  AAGCCCTACATGTGCAGGCAGTGTGGACTGGGCTTTGGCAATAAGTCAGCTCTAATTACACACAAGCGGG
  CTCACTCGGAAGAGAAGCCTTGTGTGTGCAGAGAGTGTGGCCAAGGCTTTCTCCAAAAGTCACACCTCAC
  CTTACATCAAATGACACATACGGGGGAGAAGCCATATGTGTGCAAGACGTGTGGGCGGGGCTTCAGCCTC
  AAGTCTCACCTCAGCAGACACAGGAAGACCACGTCTGTCCACCACAGACTGCCAGTGCAGCCCGACCCTG
  AGCCGTGTGCAGGGCAACCTTCGGATTCCTTATACTCTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239266 representing NM_001283007
  Red=Cloning site Green=Tags(s)
  
  MGAAGLQGAPGGAEHPGRCGPGAVPRIRGSVVLAPRWRSSPAGGWSDPLFFGGAGISFSKPELITQLEQG
  KETWREEKKCSPATCPDPEPELYLDPFCPPGFSSQKFPMQHVLCNHPPWIFTCLCAEGNIQPGDPGPGDQ
  EKQQQASEGRPWSDQAEGPEGEGAMPLFGRTKKRTLGAFSRPPQRQPVSSRNGLRGVELEASPAQTGNPE
  ETDKLLKRIEVLGFGTVNCGECGLSFSKMTNLLSHQRIHSGEKPYVCGVCEKGFSLKKSLARHQKAHSGE
  KPIVCRECGRGFNRKSTLIIHERTHSGEKPYMCSECGRGFSQKSNLIIHQRTHSGEKPYVCRECGKGFSQ
  KSAVVRHQRTHLEEKTIVCSDCGLGFSDRSNLISHQRTHSGEKPYACKECGRCFRQRTTLVNHQRTHSKE
  KPYVCGVCGHSFSQNSTLISHRRTHTGEKPYVCGVCGRGFSLKSHLNRHQNIHSGEKPIVCKDCGRGFSQ
  QSNLIRHQRTHSGEKPMVCGECGRGFSQKSNLVAHQRTHSGERPYVCRECGRGFSHQAGLIRHKRKHSRE
  KPYMCRQCGLGFGNKSALITHKRAHSEEKPCVCRECGQGFLQKSHLTLHQMTHTGEKPYVCKTCGRGFSL
  KSHLSRHRKTTSVHHRLPVQPDPEPCAGQPSDSLYSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001283007
• ORF Size: 2001 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001283007.1, NP_001269936.1
• RefSeq Size: 2360 bp
• RefSeq ORF: 2004 bp
• Locus ID: 7692
• Cytogenetics: 20p11.23
• Protein Families: Transcription Factors
• MW: 74.3 kDa
• Gene Summary: ''