ZNF133 (NM_001282996) Human Tagged ORF Clone

CAT#: RC239278

ZNF133 (myc-DDK-tagged) - Human zinc finger protein 133 (ZNF133), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF133
• Synonyms: pHZ-13; pHZ-66; ZNF150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239278 representing NM_001282996
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACACATGGCATTCAGGGATGTGGCTGTGGATTTCACCCAGGATGAGTGGAGGCTGCTGAGCCCTG
  CTCAAAGGACTCTGTACAGAGAGGTGATGCTGGAGAACTACAGCAACCTGGTCTCACTGGGAATTTCATT
  TTCTAAACCAGAACTCATCACCCAGCTGGAGCAAGGGAAAGAGACCTGGAGAGAGGAAAAAAAATGTTCA
  CCGGCAACCTGTCCAGATCATCAAGAATATGGAAACAACTGTGACCTACAGAAAGAAGCAGATCCAGAGC
  CAGAGCTCTACCTCGATCCTTTCTGCCCTCCGGGTTTCTCCAGTCAGAAATTCCCCATGCAGCATGTGCT
  GTGTAATCATCCCCCCTGGATCTTCACATGCTTGTGTGCAGAAGGTAACATCCAGCCTGGGGATCCGGGC
  CCAGGGGACCAGGAGAAGCAGCAACAAGCCTCTGAGGGGAGACCCTGGAGTGATCAAGCAGAAGGTCCTG
  AGGGAGAAGGTGCCATGCCTTTGTTTGGAAGAACCAAGAAAAGGACTCTGGGAGCGTTCTCCAGGCCACC
  CCAGAGGCAGCCAGTCAGCTCTCGGAACGGCCTCAGAGGGGTGGAGTTAGAAGCCAGCCCAGCTCAGACA
  GGGAACCCTGAGGAAACAGACAAATTGTTGAAGAGGATAGAAGTCTTAGGATTTGGAACAGTCAACTGTG
  GAGAGTGTGGACTGAGCTTCAGCAAGATGACAAACCTGCTCAGTCACCAGCGGATACACTCAGGGGAGAA
  GCCCTACGTGTGTGGGGTATGTGAGAAGGGCTTCAGCCTAAAGAAGAGCCTCGCCAGACACCAGAAGGCA
  CACTCGGGGGAGAAGCCAATTGTGTGCAGGGAGTGTGGACGAGGCTTTAACCGGAAGTCAACGCTAATCA
  TACACGAACGGACACACTCCGGTGAGAAACCTTACATGTGCAGTGAGTGTGGGCGAGGCTTCAGCCAGAA
  GTCAAACCTCATCATACACCAGAGGACACACTCAGGGGAAAAGCCTTACGTGTGCCGGGAATGTGGCAAA
  GGCTTCAGCCAGAAGTCAGCTGTCGTGAGACACCAGAGGACACACTTGGAGGAGAAGACCATCGTGTGCA
  GTGACTGTGGCCTGGGCTTCAGCGACAGGTCAAACCTCATCTCCCACCAGAGGACGCACTCTGGGGAGAA
  GCCCTACGCCTGCAAGGAGTGTGGGCGATGCTTCAGGCAGAGGACCACCCTTGTCAACCACCAGAGGACA
  CACTCAAAGGAGAAGCCCTATGTGTGCGGGGTGTGTGGGCACAGCTTCAGCCAGAATTCAACCCTCATCT
  CTCACAGGCGGACACACACTGGGGAGAAGCCGTATGTTTGTGGGGTGTGTGGGCGAGGCTTTAGTCTCAA
  GTCACACCTCAACAGACACCAGAACATACACTCAGGAGAGAAGCCCATTGTGTGCAAGGACTGTGGCCGG
  GGCTTCAGCCAGCAATCCAACCTCATCAGACACCAGAGGACGCACTCAGGCGAGAAGCCCATGGTGTGTG
  GGGAGTGCGGGCGAGGCTTCAGCCAGAAGTCAAACCTTGTTGCACACCAGAGGACGCACTCAGGGGAGAG
  GCCGTATGTGTGCCGAGAGTGCGGGCGAGGCTTTAGCCACCAGGCCGGTCTCATCAGGCACAAGCGGAAG
  CACTCGAGGGAGAAGCCCTACATGTGCAGGCAGTGTGGACTGGGCTTTGGCAATAAGTCAGCTCTAATTA
  CACACAAGCGGGCTCACTCGGAAGAGAAGCCTTGTGTGTGCAGAGAGTGTGGCCAAGGCTTTCTCCAAAA
  GTCACACCTCACCTTACATCAAATGACACATACGGGGGAGAAGCCATATGTGTGCAAGACGTGTGGGCGG
  GGCTTCAGCCTCAAGTCTCACCTCAGCAGACACAGGAAGACCACGTCTGTCCACCACAGACTGCCAGTGC
  AGCCCGACCCTGAGCCGTGTGCAGGGCAACCTTCGGATTCCTTATACTCTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239278 representing NM_001282996
  Red=Cloning site Green=Tags(s)
  
  MAHMAFRDVAVDFTQDEWRLLSPAQRTLYREVMLENYSNLVSLGISFSKPELITQLEQGKETWREEKKCS
  PATCPDHQEYGNNCDLQKEADPEPELYLDPFCPPGFSSQKFPMQHVLCNHPPWIFTCLCAEGNIQPGDPG
  PGDQEKQQQASEGRPWSDQAEGPEGEGAMPLFGRTKKRTLGAFSRPPQRQPVSSRNGLRGVELEASPAQT
  GNPEETDKLLKRIEVLGFGTVNCGECGLSFSKMTNLLSHQRIHSGEKPYVCGVCEKGFSLKKSLARHQKA
  HSGEKPIVCRECGRGFNRKSTLIIHERTHSGEKPYMCSECGRGFSQKSNLIIHQRTHSGEKPYVCRECGK
  GFSQKSAVVRHQRTHLEEKTIVCSDCGLGFSDRSNLISHQRTHSGEKPYACKECGRCFRQRTTLVNHQRT
  HSKEKPYVCGVCGHSFSQNSTLISHRRTHTGEKPYVCGVCGRGFSLKSHLNRHQNIHSGEKPIVCKDCGR
  GFSQQSNLIRHQRTHSGEKPMVCGECGRGFSQKSNLVAHQRTHSGERPYVCRECGRGFSHQAGLIRHKRK
  HSREKPYMCRQCGLGFGNKSALITHKRAHSEEKPCVCRECGQGFLQKSHLTLHQMTHTGEKPYVCKTCGR
  GFSLKSHLSRHRKTTSVHHRLPVQPDPEPCAGQPSDSLYSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282996
• ORF Size: 2013 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282996.1, NP_001269925.1
• RefSeq Size: 2610 bp
• RefSeq ORF: 2016 bp
• Locus ID: 7692
• Cytogenetics: 20p11.23
• Protein Families: Transcription Factors
• MW: 75.9 kDa
• Gene Summary: ''