STT3A (NM_001278503) Human Tagged ORF Clone

CAT#: RC239365

STT3A (myc-DDK-tagged) - Human STT3A, subunit of the oligosaccharyltransferase complex (catalytic) (STT3A), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STT3A
• Synonyms: ITM1; STT3-A; TMC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239365 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAAGTTTGGATTTTTGCGATTGTCCTATGAGAAGCAGGACACACTTTTGAAGCTTCTCATTCTGT
  CAATGGCTGCTGTATTATCCTTCTCCACTCGTCTGTTTGCTGTCCTGAGATTTGAAAGTGTTATCCATGA
  GTTTGATCCGTACTTTAATTATCGGACTACCAGGTTCCTGGCTGAGGAGGGGTTTTATAAATTCCATAAC
  TGGTTTGATGACCGAGCCTGGTACCCTTTGGGACGAATCATTGGAGGAACAATTTACCCAGGTTTAATGA
  TCACCTCTGCTGCAATCTACCATGTACTCCATTTTTTCCACATCACCATCGACATTCGGAATGTCTGTGT
  GTTCCTGGCCCCTCTCTTCTCCTCCTTCACCACCATCGTCACGTACCACCTTACCAAAGAGCTCAAGGAT
  GCAGGGGCTGGGCTTCTTGCTGCTGCCATGATTGCTGTAGTTCCTGGATATATCTCCCGATCTGTGGCTG
  GCTCCTATGATAATGAAGGGATTGCCATCTTTTGCATGCTACTCACCTACTACATGTGGATCAAGGCAGT
  AAAGACTGGTTCCATCTGTTGGGCAGCTAAGTGTGCCCTTGCTTATTTCTACATGGTCTCGTCATGGGGA
  GGTTATGTGTTCCTGATCAACTTAATTCCTCTCCACGTCCTCGTGCTGATGCTCACAGGCCGTTTCTCTC
  ACCGGATCTATGTGGCCTACTGTACTGTTTACTGCCTGGGCACTATACTTTCTATGCAGATCTCCTTTGT
  GGGTTTCCAGCCTGTCCTTTCATCAGAGCACATGGCAGCCTTTGGGGTCTTTGGTCTCTGCCAGATCCAT
  GCCTTTGTGGATTACCTGCGCAGCAAGTTGAATCCACAACAATTTGAAGTTCTTTTCCGGAGCGTCATCT
  CTCTGGTAGGCTTTGTCCTTCTCACCGTGGGAGCTCTCCTCATGCTGACAGGAAAAATATCTCCCTGGAC
  GGGGCGTTTCTACTCGCTGCTGGATCCCTCTTATGCTAAGAACAACATCCCCATCATTGCTTCTGTGTCT
  GAGCATCAGCCCACAACCTGGTCCTCATACTATTTTGACCTGCAGCTCCTCGTCTTCATGTTTCCAGTTG
  GCCTCTATTACTGCTTTAGCAACCTGTCTGATGCCCGGATTTTTATCATCATGTATGGTGTGACCAGCAT
  GTACTTTTCAGCTGTAATGGTGCGTCTAATGCTAGTGTTGGCACCTGTTATGTGCATTCTCTCTGGCATT
  GGAGTCTCCCAGGTGCTGTCCACATACATGAAGAATCTGGACATAAGTCGTCCAGACAAGAAGAGCAAGA
  AGCAACAGGATTCCACCTACCCTATTAAGAATGAAGTGGCAAGTGGGATGATACTGGTCATGGCTTTCTT
  TCTCATCACCTACACCTTTCATTCAACCTGGGTGACCAGTGAGGCCTACTCTTCTCCGTCCATTGTACTA
  TCTGCCCGTGGTGGGGATGGCAGTAGGATCATATTTGATGACTTCCGAGAAGCATATTATTGGCTTCGTC
  ATAATACTCCAGAGGATGCGAAGGTCATGTCCTGGTGGGATTATGGCTATCAGATTACAGCTATGGCAAA
  CCGAACAATTTTAGTGGACAATAACACATGGAATAATACCCATATTTCTCGAGTAGGGCAGGCAATGGCG
  TCCACAGAGGAAAAAGCCTATGAGATCATGAGGGAGCTCGATGTCAGCTATGTGCTGGTCATTTTTGGAG
  GCCTCACTGGGTATTCCTCTGATGATATCAACAAGTTTCTTTGGATGGTCCGGATTGGAGGGAGCACAGA
  TACAGGCAAACATATCAAGGAGAATGACTATTATACTCCAACTGGGGAGTTCCGTGTGGACCGTGAAGGT
  TCTCCAGTGCTGCTCAACTGCCTCATGTACAAGATGTGTTACTATCGCTTTGGACAGGTTTACACAGAAG
  CCAAGCGTCCTCCAGGCTTTGACCGTGTCCGAAATGCTGAGATTGGGAATAAAGACTTTGAGCTTGATGT
  CCTGGAGGAAGCATATACCACAGAACATTGGCTGGTCAGGATATACAAGGTAAAGGACCTGGATAATCGA
  GGCTTGTCAAGGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239365 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTKFGFLRLSYEKQDTLLKLLILSMAAVLSFSTRLFAVLRFESVIHEFDPYFNYRTTRFLAEEGFYKFHN
  WFDDRAWYPLGRIIGGTIYPGLMITSAAIYHVLHFFHITIDIRNVCVFLAPLFSSFTTIVTYHLTKELKD
  AGAGLLAAAMIAVVPGYISRSVAGSYDNEGIAIFCMLLTYYMWIKAVKTGSICWAAKCALAYFYMVSSWG
  GYVFLINLIPLHVLVLMLTGRFSHRIYVAYCTVYCLGTILSMQISFVGFQPVLSSEHMAAFGVFGLCQIH
  AFVDYLRSKLNPQQFEVLFRSVISLVGFVLLTVGALLMLTGKISPWTGRFYSLLDPSYAKNNIPIIASVS
  EHQPTTWSSYYFDLQLLVFMFPVGLYYCFSNLSDARIFIIMYGVTSMYFSAVMVRLMLVLAPVMCILSGI
  GVSQVLSTYMKNLDISRPDKKSKKQQDSTYPIKNEVASGMILVMAFFLITYTFHSTWVTSEAYSSPSIVL
  SARGGDGSRIIFDDFREAYYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMA
  STEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTDTGKHIKENDYYTPTGEFRVDREG
  SPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNR
  GLSRT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278503
• ORF Size: 2115 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278503.1, NP_001265432.1
• RefSeq Size: 4332 bp
• RefSeq ORF: 2118 bp
• Locus ID: 3703
• Cytogenetics: 11q24.2
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• MW: 80.5 kDa
• Gene Summary: 'The protein encoded by this gene is a catalytic subunit of the N-oligosaccharyltransferase (OST) complex, which functions in the endoplasmic reticulum to transfer glycan chains to asparagine residues of target proteins. A separate complex containing a similar catalytic subunit with an overlapping function also exists. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]'