TLE2 (NM_001300846) Human Tagged ORF Clone

CAT#: RC239459

  • TrueORF®

TLE2 (myc-DDK-tagged) - Human transducin-like enhancer of split 2 (TLE2), transcript variant 4


  "NM_001300846" in other vectors (1)

Reconstitution Protocol

USD 610.00

3 Weeks*

Size
    • 10 ug

Product Images

Other products for "TLE2"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TLE2
Synonyms ESG; ESG2; GRG2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC239459 representing NM_001300846
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACCCCCAGGGAAGGCACCCGACCCCGCTCCAGTCCGGCCAGCCCTTCAAGTTCTCGATCTTGGAGA
TCTGCGACCGCATCAAAGAAGAATTCCAGTTTCTTCAGGCTCAATACCACAGCCTCAAGCTAGAATGTGA
GAAGCTGGCCAGCGAGAAGACGGAAATGCAGCGACATTATGTCATGTATTATGAGATGTCGTACGGGCTC
AACATTGAAATGCATAAGCAGGCGGAGATTGTGAAGCGTCTGAGCGGTATCTGCGCTCAGATTATCCCCT
TCCTGACCCAGGAGCATCAGCAGCAGGTGCTCCAGGCCGTAGAACGCGCCAAGCAGGTCACCGTGGGGGA
GCTGAACAGCCTCATCGGGCAGCAGCAGCTCCAGCCGCTGTCCCACCACGCACCCCCTGTGCCCCTCACC
CCCCGCCCAGCCGGGCTGGTGGGCGGCAGTGCTACGGGGCTGCTTGCTCTGTCTGGAGCCCTGGCTGCCC
AGGCTCAGCTGGCGGCGGCTGTCAAGGAGGACCGTGCGGGCGTGGAGGCCGAGGGGTCCAGAGTGGAGAG
AGCCCCGAGCAGGAGTGCATCTCCCTCGCCCCCTGAGAGTCTCGTGGAGGAGGAGCGACCGAGTGGCCCT
GGTGGTGGCGGGAAGCAGAGAGCAGATGAGAAGGAGCCATCAGGACCTTATGAAAGCGACGAAGACAAGA
GTGATTACAATCTGGTGGTGGACGAGGACCAACCCTCAGAGCCCCCCAGCCCGGCTACCACCCCCTGCGG
AAAGGTACCCATCTGCATTCCTGCCCGTCGGGACCTGGTGGACAGTCCAGCCTCCTTGGCCTCTAGCCTT
GGCTCACCGCTGCCTAGAGCCAAGGAGCTCATCCTGAATGACCTTCCCGCCAGCACTCCTGCCTCCAAAT
CCTGTGACTCCTCCCCGCCCCAGGACGCTTCCACCCCCGGGCCCAGCTCGGCCAGTCACCTCTGCCAGCT
TGCTGCCAAGCCAGCACCTTCCACGGACAGCGTCGCCCTGAGGAGCCCCCTGACTCTGTCCAGTCCCTTC
ACCACGTCCTTCAGCCTGGGCTCCCACAGCACTCTCAACGGAGACCTCTCCGTGCCCAGCTCCTACGTCA
GCCTCCACCTGTCCCCCCAGGTCAGCAGCTCTGTGGTGTACGGACGCTCCCCCGTGATGGCATTTGAGTC
TCATCCCCATCTCCGAGGGTCATCCGTCTCTTCCTCCCTACCCAGCATCCCTGGGGGAAAGCCGGCCTAC
TCCTTCCACGTGTCTGCGGACGGGCAGATGCAGCCGGTTCCCTTCCCCTCGGATGCACTGGTAGGCGCGG
GCATCCCGCGGCACGCCCGGCAGCTGCACACGCTGGCCCATGGCGAGGTGGTCTGCGCGGTCACCATCAG
CGGCTCCACACAGCATGTGTACACGGGCGGCAAGGGCTGTGTGAAGGTGTGGGACGTGGGCCAGCCTGGG
GCCAAGACGCCCGTGGCCCAGCTCGACTGCCTGAACCGAGACAACTACATTCGTTCCTGCAAGTTGCTGC
CGGATGGCCGGAGTCTGATCGTGGGCGGTGAGGCCAGCACCTTGTCCATTTGGGACCTGGCGGCGCCCAC
CCCCCGTATCAAGGCCGAGCTGACTTCCTCAGCCCCAGCCTGCTACGCCCTGGCCGTCAGCCCCGACGCC
AAGGTTTGCTTCTCCTGCTGCAGCGATGGCAACATTGTGGTCTGGGACCTGCAGAATCAGACTATGGTCA
GGCAGTTCCAGGGCCACACGGACGGCGCCAGCTGCATTGATATTTCCGATTACGGCACTCGGCTCTGGAC
AGGGGGCCTGGACAACACGGTGCGCTGCTGGGACCTGCGGGAGGGCCGCCAGCTGCAGCAGCATGACTTC
AGCTCCCAGATTTTCTCCCTGGGCCACTGCCCTAACCAGGACTGGCTGGCGGTCGGAATGGAGAGTAGCA
ACGTGGAGATCCTGCACGTCCGCAAGCCGGAGAAATACCAGCTGCACCTCCACGAGAGCTGCGTGCTGTC
CCTGAAGTTTGCCTCCTGCGGACGGTGGTTTGTGAGCACCGGGAAGGACAACCTGCTCAACGCCTGGAGG
ACGCCGTACGGGGCCAGCATTTTCCAGTCCAAGGAGTCGTCCTCAGTCCTGAGTTGTGACATCTCCAGAA
ATAACAAATACATCGTGACAGGCTCGGGGGACAAGAAGGCCACCGTGTATGAGGTGGTCTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC239459 representing NM_001300846
Red=Cloning site Green=Tags(s)

MYPQGRHPTPLQSGQPFKFSILEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGL
NIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGQQQLQPLSHHAPPVPLT
PRPAGLVGGSATGLLALSGALAAQAQLAAAVKEDRAGVEAEGSRVERAPSRSASPSPPESLVEEERPSGP
GGGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICIPARRDLVDSPASLASSL
GSPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAPSTDSVALRSPLTLSSPF
TTSFSLGSHSTLNGDLSVPSSYVSLHLSPQVSSSVVYGRSPVMAFESHPHLRGSSVSSSLPSIPGGKPAY
SFHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHVYTGGKGCVKVWDVGQPG
AKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAVSPDA
KVCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDF
SSQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASCGRWFVSTGKDNLLNAWR
TPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001300846
ORF Size 2232 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001300846.1, NP_001287775.1
RefSeq Size 2759 bp
RefSeq ORF 2235 bp
Locus ID 7089
Cytogenetics 19p13.3
Protein Families Transcription Factors
MW 80.4 kDa
Gene Summary ''

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.