GPRC6A (NM_001286354) Human Tagged ORF Clone

CAT#: RC239482

GPRC6A (myc-DDK-tagged) - Human G protein-coupled receptor, class C, group 6, member A (GPRC6A), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPRC6A
• Synonyms: bA86F4.3; GPCR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239482 representing NM_001286354
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATTCTTAATTATACTAATTACCTGCTTTGTGATTATTCTTGCTACTTCACAGCCTTGCCAGACCC
  CTGATGACTTTGTGGCTGCCACTTCTCCGGGACATATCATAATTGGAGGTTTGTTTGCTATTCATGAAAA
  AATGTTGTCCTCAGAAGACTCTCCCAGACGACCACAAATCCAGGAGTGTGTTGGCTTTGAAATATCAGTT
  TTTCTTCAAACTCTTGCCATGATACACAGCATTGAGATGATCAACAATTCAACACTCTTACCTGGAGTCA
  AACTGGGGTATGAAATCTATGACACTTGTACAGAAGTCACAGTGGCAATGGCAGCCACTCTGAGGTTTCT
  TTCTAAATTCAACTGCTCCAGAGAAACTGTGGAGTTTAAGTGTGACTATTCCAGCTACATGCCAAGAGTT
  AAGGCTGTCATAGGTTCTGGGTACTCAGAAATAACTATGGCTGTCTCCAGGATGTTGAATTTACAGCTCA
  TGCCACAGGTGGGTTATGAATCAACTGCAGAAATCCTGAGTGACAAAATTCGCTTTCCTTCATTTTTACG
  GACTGTGCCCAGTGACTTCCATCAAATTAAAGCAATGGCTCACCTGATTCAGAAATCTGGTTGGAACTGG
  ATTGGCATCATAACCACAGATGATGACTATGGACGATTGGCTCTTAACACTTTTATAATTCAGGCTGAAG
  CAAATAACGTGTGCATAGCCTTCAAAGAGGTTCTTCCAGCCTTTCTTTCAGATAATACCATTGAAGTCAG
  AATCAATCGGACACTGAAGAAAATCATTTTAGAAGCCCAGTTACTTGGTGTGCTAAAAAATGTGACATTC
  ACTGATGGATGGAATTCATTTCATTTTGATGCTCACGGGGATTTAAATACTGGATATGATGTTGTGCTCT
  GGAAGGAGATCAATGGACACATGACTGTCACTAAGATGGCAGAATATGACCTACAGAATGATGTCTTCAT
  CATCCCAGATCAGGAAACAAAAAATGAGTTCAGGAATCTTAAGCAAATTCAATCTAAATGCTCCAAGGAA
  TGCAGTCCTGGGCAAATGAAGAAAACTACAAGAAGTCAACACATCTGTTGCTATGAATGTCAGAACTGTC
  CTGAAAATCATTACACTAATCAGACAGATATGCCTCACTGCCTTTTATGCAACAACAAAACTCACTGGGC
  CCCTGTTAGGAGCACTATGTGCTTTGAAAAGGAAGTGGAATATCTCAACTGGAATGACTCCTTGGCCATC
  CTACTCCTGATTCTCTCCCTACTGGGAATCATATTTGTTCTGGTTGTTGGCATAATATTTACAAGAAACC
  TGAACACACCTGTTGTGAAATCATCCGGGGGATTAAGAGTCTGCTATGTGATCCTTCTCTGTCATTTCCT
  CAATTTTGCCAGCACGAGCTTTTTCATTGGAGAACCACAAGACTTCACATGTAAAACCAGGCAGACAATG
  TTTGGAGTGAGCTTTACTCTTTGCATCTCCTGCATTTTGACGAAGTCTCTGAAAATTTTGCTAGCCTTCA
  GCTTTGATCCCAAATTACAGAAATTTCTGAAGTGCCTCTATAGACCGATCCTTATTATCTTCACTTGCAC
  GGGCATCCAGGTTGTCATTTGCACACTCTGGCTAATCTTTGCAGCACCTACTGTAGAGGTGAATGTCTCC
  TTGCCCAGAGTCATCATCCTGGAGTGTGAGGAGGGATCCATACTTGCATTTGGCACCATGCTGGGCTACA
  TTGCCATCCTGGCCTTCATTTGCTTCATATTTGCTTTCAAAGGCAAATATGAGAATTACAATGAAGCCAA
  ATTCATTACATTTGGCATGCTCATTTACTTCATAGCTTGGATCACATTCATCCCTATCTATGCTACCACA
  TTTGGCAAATATGTACCAGCTGTGGAGATTATTGTCATATTAATATCTAACTATGGAATCCTGTATTGCA
  CATTCATCCCCAAATGCTATGTTATTATTTGTAAGCAAGAGATTAACACAAAGTCTGCCTTTCTCAAGAT
  GATCTACAGTTATTCTTCCCATAGTGTGAGCAGCATTGCCCTGAGTCCTGCTTCACTGGACTCCATGAGC
  GGCAATGTCACAATGACCAATCCCAGCTCTAGTGGCAAGTCTGCAACCTGGCAGAAAAGCAAAGATCTTC
  AGGCACAAGCATTTGCACACATATGCAGGGAAAATGCCACAAGTGTATCTAAAACTTTGCCTCGAAAAAG
  AATGTCAAGTATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239482 representing NM_001286354
  Red=Cloning site Green=Tags(s)
  
  MAFLIILITCFVIILATSQPCQTPDDFVAATSPGHIIIGGLFAIHEKMLSSEDSPRRPQIQECVGFEISV
  FLQTLAMIHSIEMINNSTLLPGVKLGYEIYDTCTEVTVAMAATLRFLSKFNCSRETVEFKCDYSSYMPRV
  KAVIGSGYSEITMAVSRMLNLQLMPQVGYESTAEILSDKIRFPSFLRTVPSDFHQIKAMAHLIQKSGWNW
  IGIITTDDDYGRLALNTFIIQAEANNVCIAFKEVLPAFLSDNTIEVRINRTLKKIILEAQLLGVLKNVTF
  TDGWNSFHFDAHGDLNTGYDVVLWKEINGHMTVTKMAEYDLQNDVFIIPDQETKNEFRNLKQIQSKCSKE
  CSPGQMKKTTRSQHICCYECQNCPENHYTNQTDMPHCLLCNNKTHWAPVRSTMCFEKEVEYLNWNDSLAI
  LLLILSLLGIIFVLVVGIIFTRNLNTPVVKSSGGLRVCYVILLCHFLNFASTSFFIGEPQDFTCKTRQTM
  FGVSFTLCISCILTKSLKILLAFSFDPKLQKFLKCLYRPILIIFTCTGIQVVICTLWLIFAAPTVEVNVS
  LPRVIILECEEGSILAFGTMLGYIAILAFICFIFAFKGKYENYNEAKFITFGMLIYFIAWITFIPIYATT
  FGKYVPAVEIIVILISNYGILYCTFIPKCYVIICKQEINTKSAFLKMIYSYSSHSVSSIALSPASLDSMS
  GNVTMTNPSSSGKSATWQKSKDLQAQAFAHICRENATSVSKTLPRKRMSSI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286354
• ORF Size: 2253 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001286354.1, NP_001273283.1
• RefSeq Size: 2357
• RefSeq ORF: 2256
• Locus ID: 222545
• Protein Families: Druggable Genome, Transmembrane
• MW: 85 kDa
• Gene Summary: Members of family C of the G protein-coupled receptor (GPCR) superfamily, such as GPRC6A, are characterized by an evolutionarily conserved amino acid-sensing motif linked to an intramembranous 7-transmembrane loop region. Several members of GPCR family C, including GPRC6A, also have a long N-terminal domain (summary by Pi et al., 2005 [PubMed 16199532]). [supplied by OMIM, Nov 2010]