Stromal interaction molecule 1 (STIM1) (NM_001277961) Human Tagged ORF Clone
CAT#: RC239573
- TrueORF®
STIM1 (myc-DDK-tagged) - Human stromal interaction molecule 1 (STIM1), transcript variant 1
"NM_001277961" in other vectors (1)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | STIM1 |
Synonyms | D11S4896E; GOK; IMD10; STRMK; TAM; TAM1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239573 representing NM_001277961
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTATGCGTCCGTCTTGCCCTGTGGCTCCTCTGGGGACTCCTCCTGCACCAGGGCCAGAGCCTCA GCCATAGTCACAGTGAGAAGGCGACAGGAACCAGCTCGGGGGCCAACTCTGAGGAGTCCACTGCAGCAGA GTTTTGCCGAATTGACAAGCCCCTGTGTCACAGTGAGGATGAGAAACTCAGCTTCGAGGCAGTCCGTAAC ATCCACAAACTGATGGACGATGATGCCAATGGTGATGTGGATGTGGAAGAAAGTGATGAGTTCCTGAGGG AAGACCTCAATTACCATGACCCAACAGTGAAACACAGCACCTTCCATGGTGAGGATAAGCTCATCAGCGT GGAGGACCTGTGGAAGGCATGGAAGTCATCAGAAGTATACAATTGGACCGTGGATGAGGTGGTACAGTGG CTGATCACATATGTGGAGCTGCCTCAGTATGAGGAGACCTTCCGGAAGCTGCAGCTCAGTGGCCATGCCA TGCCAAGGCTGGCTGTCACCAACACCACCATGACAGGGACTGTGCTGAAGATGACAGACCGGAGTCATCG GCAGAAGCTGCAGCTGAAGGCTCTGGATACAGTGCTCTTTGGGCCTCCTCTCTTGACTCGCCATAATCAC CTCAAGGACTTCATGCTGGTGGTGTCTATCGTTATTGGTGTGGGCGGCTGCTGGTTTGCCTATATCCAGA ACCGTTACTCCAAGGAGCACATGAAGAAGATGATGAAGGACTTGGAGGGGTTACACCGAGCTGAGCAGAG TCTGCATGACCTTCAGGAAAGGCTGCACAAGGCCCAGGAGGAGCACCGCACAGTGGAGGTGGAGAAGGTC CATCTGGAAAAGAAGCTGCGCGATGAGATCAACCTTGCTAAGCAGGAAGCCCAGCGGCTGAAGGAGCTGC GGGAGGGTACTGAGAATGAGCGGAGCCGCCAAAAATATGCTGAGGAGGAGTTGGAGCAGGTTCGGGAGGC CTTGAGGAAAGCAGAGAAGGAGCTAGAATCTCACAGCTCATGGTATGCTCCAGAGGCCCTTCAGAAGTGG CTGCAGCTGACACATGAGGTGGAGGTGCAATATTACAACATCAAGAAGCAAAATGCTGAGAAGCAGCTGC TGGTGGCCAAGGAGGGGGCTGAGAAGATAAAAAAGAAGAGAAACACACTCTTTGGCACCTTCCACGTGGC CCACAGCTCTTCCCTGGATGATGTAGATCATAAAATTCTAACAGCTAAGCAAGCACTGAGCGAGGTGACA GCAGCATTGCGGGAGCGCCTGCACCGCTGGCAACAGATCGAGATCCTCTGTGGCTTCCAGATTGTCAACA ACCCTGGCATCCACTCACTGGTGGCTGCCCTCAACATAGACCCCAGCTGGATGGGCAGTACACGCCCCAA CCCTGCTCACTTCATCATGACTGACGACGTGGATGACATGGATGAGGAGATTGTGTCTCCCTTGTCCATG CAGTCCCCTAGCCTGCAGAGCAGTGTTCGGCAGCGCCTGACGGAGCCACAGCATGGCCTGGGATCTCAGA GGTTGGTAGAGGGCGAGGCTGGCCACTTCTTGACAAGCCGGGTATCTCTGCGGCGAATGCGCAGCCTTTC ATCTGGACAGTCTTTCAGTTCTGAAGGCTACGGGACCAGCTCTCCATCTGCCTCTGCTGCTGCTTCTTGC TCCTCTTCCATCACCACCATCACCACTACCACCACCACCACCACCACCTTCACCACCGTCCATGTCCACC CTGTTTATTACCACCACAGCACTTCCTATTTCCTCCAGATGGAGCCCTACCCTGACACACCCCCTTCTGA CAGCACCGCTGTGATGCCTGGGCATTCAGAGAGCTTGGGGGATTTGACCCATTCCGATTCGGAGTCCTCC CTCCACATGAGTGACCGCCAGCGTGTGGCCCCCAAACCTCCTCAGATGAGCCGTGCTGCAGACGAGGCTC TCAATGCCATGACTTCCAATGGCAGCCACCGGCTGATCGAGGGGGTCCACCCAGGGTCTCTGGTGGAGAA ACTGCCTGACAGCCCTGCCCTGGCCAAGAAGGCATTACTGGCGCTGAACCATGGGCTGGACAAGGCCCAC AGCCTGATGGAGCTGAGCCCCTCAGCCCCACCTGGTGGCTCTCCACATTTGGATTCTTCCCGTTCTCACA GCCCCAGCTCCCCAGACCCAGACACACCATCTCCAGTTGGGGACAGCCGAGCCCTGCAAGCCAGCCGAAA CACACGCATTCCCCACCTGGCTGGCAAGAAGGCTGTGGCTGAGGAGGATAATGGCTCTATTGGCGAGGAA ACAGACTCCAGCCCAGGCCGGAAGAAGTTTCCCCTCAAAATCTTTAAGAAGCCTCTTAAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239573 representing NM_001277961
Red=Cloning site Green=Tags(s) MDVCVRLALWLLWGLLLHQGQSLSHSHSEKATGTSSGANSEESTAAEFCRIDKPLCHSEDEKLSFEAVRN IHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQW LITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNH LKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKV HLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKW LQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVT AALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSM QSPSLQSSVRQRLTEPQHGLGSQRLVEGEAGHFLTSRVSLRRMRSLSSGQSFSSEGYGTSSPSASAAASC SSSITTITTTTTTTTTFTTVHVHPVYYHHSTSYFLQMEPYPDTPPSDSTAVMPGHSESLGDLTHSDSESS LHMSDRQRVAPKPPQMSRAADEALNAMTSNGSHRLIEGVHPGSLVEKLPDSPALAKKALLALNHGLDKAH SLMELSPSAPPGGSPHLDSSRSHSPSSPDPDTPSPVGDSRALQASRNTRIPHLAGKKAVAEEDNGSIGEE TDSSPGRKKFPLKIFKKPLKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001277961 |
ORF Size | 2373 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001277961.1, NP_001264890.1 |
RefSeq Size | 4380 bp |
RefSeq ORF | 2376 bp |
Locus ID | 6786 |
Cytogenetics | 11p15.4 |
Protein Families | Transmembrane |
MW | 89.1 kDa |
Gene Summary | 'This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC337467 | STIM1 (untagged) - Human stromal interaction molecule 1 (STIM1), transcript variant 1 |
USD 790.00 |
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