CRNKL1 (NM_001278625) Human Tagged ORF Clone

CAT#: RC239653

CRNKL1 (myc-DDK-tagged) - Human crooked neck pre-mRNA splicing factor 1 (CRNKL1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRNKL1
• Synonyms: CLF; Clf1; CRN; HCRN; MSTP021; SYF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239653 representing NM_001278625
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGCAACAGTGGAGAACCTAACGTTCCAGAAGGACACTTTGGGAAATGCTGTTGACAAAAACAGGC
  GCCACGGCTCGACAGAGCCACTCGTCCTCGCCTGGTCATCCCAGTTCCGGAGGCTGACCTGGGGATGCGC
  CCTGGACGCCCTGCACCGCAGTCCTTGCGTCGCTGCTAGTCAGCACGGCGTTACCCACCTCATCCGCAGC
  TCCAGGACGCCGCACTCCACGCGCTGCCGCAAGGAGGACGCGCAGCCTGGTCACCATGGAAACGGTGCCG
  CCTCCGTCACGGCTCAAGCGAGAGGTCAAAGGTCAGTCCTGCAAGTTCCGCTTCCGGTTCCGAGATCCTG
  TTTGTTTTCTGAAAGCTTTGTGGTTTCGGTGAGCTCTCAGAGCCGATTTCTAGCGTCCGTGCCGGGGACA
  GGTGTCCAGAGGTCGACTGCTGCAGACATGGCGGCCTCCACCGCGGCCGGGAAGCAGCGGATTCCCAAAG
  TGGCCAAGGTGAAAAACAAAGCCCCGGCTGAGGTACAGATAACTGCTGAACAACTCTTAAGAGAGGCTAA
  AGAAAGAGAACTTGAGCTTCTTCCACCTCCACCTCAACAGAAGATCACAGATGAAGAAGAATTAAATGAT
  TATAAACTAAGGAAAAGGAAGACTTTTGAAGATAATATAAGAAAAAACAGGACTGTGATTAGTAACTGGA
  TAAAATACGCACAATGGGAAGAAAGCCTAAAGGAGATTCAAAGGGCTCGATCCATATACGAGCGTGCTTT
  AGATGTAGACTACCGAAATATTACACTCTGGCTGAAATACGCAGAAATGGAAATGAAGAATCGCCAAGTC
  AACCATGCTCGAAATATCTGGGACCGGGCCATAACAACGCTGCCTCGAGTTAATCAGTTCTGGTACAAGT
  ACACGTACATGGAGGAAATGTTGGGAAACGTTGCCGGTGCCCGGCAGGTGTTTGAGCGCTGGATGGAGTG
  GCAGCCTGAGGAGCAAGCCTGGCACTCCTACATCAACTTTGAGCTGAGATACAAAGAGGTGGATCGGGCC
  CGCACCATTTATGAGCGATTTGTCCTCGTGCACCCTGATGTTAAGAACTGGATCAAGTATGCCCGCTTTG
  AAGAAAAACATGCTTATTTTGCCCATGCACGGAAAGTGTATGAGAGAGCTGTGGAATTCTTTGGAGATGA
  ACATATGGATGAGCACCTTTATGTTGCCTTTGCCAAGTTTGAAGAAAATCAGAAAGAGTTTGAAAGGGTA
  CGAGTGATTTACAAGTATGCCCTGGACAGAATTTCAAAACAAGATGCCCAAGAACTCTTTAAAAATTATA
  CCATCTTTGAGAAGAAGTTTGGTGATAGGCGGGGTATTGAAGATATCATTGTGAGCAAACGGAGATTCCA
  GTACGAAGAAGAAGTGAAGGCGAATCCACACAATTATGATGCATGGTTTGATTACTTGCGCTTGGTAGAA
  AGTGACGCAGAAGCTGAAGCCGTGAGAGAAGTCTATGAAAGGGCCATTGCCAATGTCCCACCCATTCAGG
  AGAAGAGGCACTGGAAGCGCTACATTTATCTTTGGATCAACTATGCACTCTATGAAGAATTGGAGGCAAA
  GGATCCTGAGAGGACAAGACAGGTGTATCAAGCCTCTTTGGAACTAATTCCTCACAAAAAGTTCACATTT
  GCCAAAATGTGGATACTGTATGCACAGTTTGAAATACGACAGAAGAATCTGTCATTAGCCAGAAGAGCAT
  TGGGAACTTCCATAGGCAAATGTCCAAAGAACAAATTATTTAAAGTTTACATAGAATTGGAGCTACAGCT
  TCGAGAATTTGACAGATGCCGGAAGCTTTATGAAAAGTTCCTGGAATTTGGACCTGAAAATTGTACCTCA
  TGGATTAAATTCGCTGAATTAGAGACAATCCTTGGTGATATTGACAGAGCACGGGCAATCTATGAATTAG
  CCATCAGTCAGCCACGTTTAGACATGCCAGAGGTGCTTTGGAAATCATATATTGATTTTGAAATTGAGCA
  GGAAGAAACAGAAAGAACACGAAACCTTTACCGGCGGTTGCTTCAACGGACGCAGCATGTCAAGGTATGG
  ATCAGCTTTGCTCAGTTTGAGTTGTCTTCAGGAAAAGAAGGAAGTTTGACTAAATGCAGACAAATTTATG
  AAGAAGCTAACAAAACCATGCGAAACTGTGAAGAAAAGGAAGAGAGACTTATGCTGCTGGAATCTTGGCG
  AAGTTTTGAAGAAGAATTTGGAACAGCTTCAGATAAGGAGAGAGTAGACAAACTCATGCCAGAGAAAGTC
  AAGAAGAGAAGAAAGGTCCAGACTGATGATGGGTCTGATGCAGGCTGGGAAGAATACTTTGATTACATCT
  TTCCAGAAGATGCTGCCAACCAACCTAACCTCAAACTCCTGGCCATGGCCAAACTGTGGAAGAAACAGCA
  GCAGGAAAAGGAGGATGCTGAGCACCATCCAGATGAGGACGTCGATGAGAGTGAATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239653 representing NM_001278625
  Red=Cloning site Green=Tags(s)
  
  MTATVENLTFQKDTLGNAVDKNRRHGSTEPLVLAWSSQFRRLTWGCALDALHRSPCVAASQHGVTHLIRS
  SRTPHSTRCRKEDAQPGHHGNGAASVTAQARGQRSVLQVPLPVPRSCLFSESFVVSVSSQSRFLASVPGT
  GVQRSTAADMAASTAAGKQRIPKVAKVKNKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELND
  YKLRKRKTFEDNIRKNRTVISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQV
  NHARNIWDRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYKEVDRA
  RTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFAKFEENQKEFERV
  RVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQYEEEVKANPHNYDAWFDYLRLVE
  SDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINYALYEELEAKDPERTRQVYQASLELIPHKKFTF
  AKMWILYAQFEIRQKNLSLARRALGTSIGKCPKNKLFKVYIELELQLREFDRCRKLYEKFLEFGPENCTS
  WIKFAELETILGDIDRARAIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQRTQHVKVW
  ISFAQFELSSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERVDKLMPEKV
  KKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPDEDVDESES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278625
• ORF Size: 2508 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001278625.1, NP_001265554.1
• RefSeq Size: 4377
• RefSeq ORF: 2511
• Locus ID: 51340
• Protein Pathways: Spliceosome
• MW: 99.6 kDa
• Gene Summary: The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013]