NELL1 (NM_001288713) Human Tagged ORF Clone

CAT#: RC239659

NELL1 (myc-DDK-tagged) - Human NEL-like 1 (chicken) (NELL1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NELL1
• Synonyms: IDH3GL; NRP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239659 representing NM_001288713
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGATGGATTTGATTTTAGTTGTGTGGTTCTGTGTGTGCACTGCCAGGACAGCAGAAGATATGAAGC
  CACCCGTGTTATCGCTGATATGGAGAACTAGAGTTCTGAAGTCTCTGCTTCAGCAATCCCTTCAGGGAGT
  GGTGGGCTTTGGGATGGACCCTGACCTTCAGATGGATATCGTCACCGAGCTTGACCTTGTGAACACCACC
  CTTGGAGTTGCTCAGGTGTCTGGAATGCACAATGCCAGCAAAGCATTTTTATTTCAAGACATAGAAAGAG
  AGATCCATGCAGCTCCTCATGTGAGTGAGAAATTAATTCAGCTGTTCCGGAACAAGAGTGAATTCACCAT
  TTTGGCCACTGTACAGCAGAAGCCATCCACTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGC
  TATTTTGAACTGGAGAGCAGTGGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAGCCAA
  GGACAGAGGCACTTCCTTACCGCATGGCAGATGGACAATGGCACAAGGTTGCACTGTCAGTTAGCGCCTC
  TCATCTCCTGCTCCATGTCGACTGTAACAGGATTTATGAGCGTGTGATAGACCCTCCAGATACCAACCTT
  CCCCCAGGAATCAATTTATGGCTTGGCCAGCGCAACCAAAAGCATGGCTTATTCAAAGGGATCATCCAAG
  ATGGGAAGATCATCTTTATGCCGAATGGATATATAACACAGTGTCCAAATCTAAATCACACTTGCCCAAC
  CTGCAGTGATTTCTTAAGCCTGGTGCAAGGAATAATGGATTTACAAGAGCTTTTGGCCAAGATGACTGCA
  AAACTAAATTATGCAGAGACAAGACTTAGTCAATTGGAAAACTGTCATTGTGAGAAGACTTGTCAAGTGA
  GTGGACTGCTCTATCGAGATCAAGACTCTTGGGTAGATGGTGACCATTGCAGGAACTGCACTTGCAAAAG
  TGGTGCCGTGGAATGCCGAAGGATGTCCTGTCCCCCTCTCAATTGCTCCCCAGACTCCCTCCCAGTGCAC
  ATTGCTGGCCAGTGCTGTAAGGTCTGCCGACCAAAATGTATCTATGGAGGAAAAGTTCTTGCAGAAGGCC
  AGCGGATTTTAACCAAGAGCTGTCGGGAATGCCGAGGTGGAGTTTTAGTAAAAATTACAGAAATGTGTCC
  TCCTTTGAACTGCTCAGAAAAGGATCACATTCTTCCTGAGAATCAGTGCTGCCGTGTCTGTAGAGGTCAT
  AACTTTTGTGCAGAAGGACCTAAATGTGGTGAAAACTCAGAGTGCAAAAACTGGAATACAAAAGCTACTT
  GTGAGTGCAAGAGTGGTTACATCTCTGTCCAGGGAGACTCTGCCTACTGTGAAGATATTGATGAGTGTGC
  AGCTAAGATGCATTACTGTCATGCCAATACTGTGTGTGTCAACCTTCCTGGGTTATATCGCTGTGACTGT
  GTCCCAGGATACATTCGTGTGGATGACTTCTCTTGTACAGAACACGATGAATGTGGCAGCGGCCAGCACA
  ACTGTGATGAGAATGCCATCTGCACCAACACTGTCCAGGGACACAGCTGCACCTGCAAACCGGGCTACGT
  GGGGAACGGGACCATCTGCAGAGCTTTCTGTGAAGAGGGCTGCAGATACGGTGGAACGTGTGTGGCTCCC
  AACAAATGTGTCTGTCCATCTGGATTCACAGGAAGCCACTGCGAGAAAGATATTGATGAATGTTCAGAGG
  GAATCATTGAGTGCCACAACCATTCCCGCTGCGTTAACCTGCCAGGGTGGTACCACTGTGAGTGCAGAAG
  CGGTTTCCATGACGATGGGACCTATTCACTGTCCGGGGAGTCCTGTATTGACATTGATGAATGTGCCTTA
  AGAACTCACACCTGTTGGAACGATTCTGCCTGCATCAACCTGGCAGGGGGCTTTGACTGTCTCTGCCCCT
  CTGGGCCCTCCTGCTCTGGTGACTGTCCTCATGAAGGGGGGCTGAAGCACAATGGCCAGGTGTGGACCTT
  GAAAGAAGACAGGTGTTCTGTCTGCTCCTGCAAGGATGGCAAGATATTCTGCCGACGGACAGCTTGTGAT
  TGCCAGAATCCAAGTGCTGACCTATTCTGTTGCCCAGAATGTGACACCAGAGTCACAAGTCAATGTTTAG
  ACCAAAATGGTCACAAGCTGTATCGAAGTGGAGACAATTGGACCCATAGCTGTCAGCAGTGTCGGTGTCT
  GGAAGGAGAGGTAGATTGCTGGCCACTCACTTGCCCCAACTTGAGCTGTGAGTATACAGCTATCTTAGAA
  GGGGAATGTTGTCCCCGCTGTGTCAGTGACCCCTGCCTAGCTGATAACATCACCTATGACATCAGAAAAA
  CTTGCCTGGACAGCTATGGTGTTTCACGGCTTAGTGGCTCAGTGTGGACGATGGCTGGATCTCCCTGCAC
  AACCTGTAAATGCAAGAATGGAAGAGTCTGTTGTTCTGTGGATTTTGAGTGTCTTCAAAATAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239659 representing NM_001288713
  Red=Cloning site Green=Tags(s)
  
  MPMDLILVVWFCVCTARTAEDMKPPVLSLIWRTRVLKSLLQQSLQGVVGFGMDPDLQMDIVTELDLVNTT
  LGVAQVSGMHNASKAFLFQDIEREIHAAPHVSEKLIQLFRNKSEFTILATVQQKPSTSGVILSIRELEHS
  YFELESSGLRDEIRYHYIHNGKPRTEALPYRMADGQWHKVALSVSASHLLLHVDCNRIYERVIDPPDTNL
  PPGINLWLGQRNQKHGLFKGIIQDGKIIFMPNGYITQCPNLNHTCPTCSDFLSLVQGIMDLQELLAKMTA
  KLNYAETRLSQLENCHCEKTCQVSGLLYRDQDSWVDGDHCRNCTCKSGAVECRRMSCPPLNCSPDSLPVH
  IAGQCCKVCRPKCIYGGKVLAEGQRILTKSCRECRGGVLVKITEMCPPLNCSEKDHILPENQCCRVCRGH
  NFCAEGPKCGENSECKNWNTKATCECKSGYISVQGDSAYCEDIDECAAKMHYCHANTVCVNLPGLYRCDC
  VPGYIRVDDFSCTEHDECGSGQHNCDENAICTNTVQGHSCTCKPGYVGNGTICRAFCEEGCRYGGTCVAP
  NKCVCPSGFTGSHCEKDIDECSEGIIECHNHSRCVNLPGWYHCECRSGFHDDGTYSLSGESCIDIDECAL
  RTHTCWNDSACINLAGGFDCLCPSGPSCSGDCPHEGGLKHNGQVWTLKEDRCSVCSCKDGKIFCRRTACD
  CQNPSADLFCCPECDTRVTSQCLDQNGHKLYRSGDNWTHSCQQCRCLEGEVDCWPLTCPNLSCEYTAILE
  GECCPRCVSDPCLADNITYDIRKTCLDSYGVSRLSGSVWTMAGSPCTTCKCKNGRVCCSVDFECLQNN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288713
• ORF Size: 2514 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001288713.1, NP_001275642.1
• RefSeq Size: 3354 bp
• RefSeq ORF: 2517 bp
• Locus ID: 4745
• Cytogenetics: 11p15.1
• Protein Families: Secreted Protein
• MW: 93.3 kDa
• Gene Summary: 'This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]'