Epac2 (RAPGEF4) (NM_001282900) Human Tagged ORF Clone

CAT#: RC239661

RAPGEF4 (myc-DDK-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RAPGEF4
• Synonyms: CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2; Nbla00496
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239661 representing NM_001282900
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGACTTCTGGCTCCTCCTTATGGTGTTATGGAAACGGGCTCTAACAATGACAGGATTCCTGACA
  AGGAGAACGTCCCTTCAGAGAAGATCCTCAGAGCTGGAAAAATTTTACGAAATGCCATTCTCTCTCGAGC
  ACCTCACATGATAAGAGATAGAAAATACCACCTAAAGACATACAGACAATGCTGTGTGGGAACTGAACTG
  GTGGACTGGATGATGCAGCAGACACCATGTGTTCACTCCCGGACTCAAGCTGTTGGCATGTGGCAAGTCC
  TGTTAGAAGATGGTGTTCTCAACCACGTGGACCAGGAGCACCATTTCCAAGACAAATATTTATTCTATCG
  ATTTCTGGATGATGAGCACGAGGATGCCCCTTTGCCTACTGAGGAGGAGAAGAAGGAGTGTGATGAGGAG
  CTCCAGGACACCATGCTGCTGCTGTCACAGATGGGCCCCGACGCCCACATGAGGATGATCCTTCGCAAAC
  CACCTGGCCAGAGGACTGTGGATGACCTAGAGATTATCTATGAGGAGCTTCTTCATATTAAAGCCTTATC
  CCATCTTTCTACCACAGTGAAACGAGAGTTAGCAGGTGTTCTCATTTTTGAGTCTCACGCCAAAGGAGGG
  ACTGTGTTGTTTAACCAGGGGGAAGAAGGTACCTCCTGGTACATTATTCTAAAAGGATCAGTGAATGTAG
  TCATTTACGGCAAGGGTGTGGTCTGCACCCTGCATGAAGGAGATGACTTCGGCAAGTTAGCACTAGTGAA
  TGATGCCCCACGAGCTGCCTCTATCGTCTTACGAGAAGATAACTGCCATTTCTTAAGAGTAGACAAGGAG
  GATTTCAACCGGATCCTAAGGGACGTGGAGGCGAATACAGTCAGACTTAAAGAACATGACCAAGATGTCT
  TGGTGCTGGAGAAGGTCCCAGCAGGGAACAGAGCTTCTAATCAAGGAAACTCACAGCCTCAGCAAAAGTA
  TACTGTGATGTCAGGAACACCTGAAAAAATTTTAGAGCATTTTCTAGAAACAATACGCCTTGAGGCAACT
  TTAAATGAAGCAACAGATTCTGTTTTAAATGACTTTATTATGATGCACTGTGTTTTTATGCCAAATACCC
  AGCTTTGCCCGGCACTGGTGGCCCACTACCACGCACAGCCTTCACAAGGTACAGAACAGGAGAAAATGGA
  TTATGCCCTCAACAATAAGAGGCGAGTCATCCGCCTGGTTCTACAGTGGGCTGCCATGTATGGAGACCTC
  CTGCAAGAGGATGACGTGTCTATGGCCTTCCTGGAGGAGTTTTATGTATCTGTATCAGATGATGCCCGGA
  TGATTGCTGCCCTCAAGGAGCAACTGCCAGAGTTGGAGAAGATTGTCAAGCAAATCTCAGAAGATGCAAA
  GGCACCACAAAAGAAGCACAAGGTTCTTTTGCAACAGTTCAATACGGGCGATGAGAGAGCCCAGAAGCGC
  CAGCCTATCCGCGGCTCTGATGAAGTTCTGTTTAAGGTCTATTGCATGGACCACACCTACACAACCATTC
  GGGTGCCAGTGGCCACTTCGGTGAAGGAAGTCATCAGTGCAGTTGCCGACAAGCTGGGCTCCGGGGAGGG
  CCTGATCATAGTCAAGATGAGTTCCGGAGGAGAAAAGGTGGTGCTCAAACCTAATGATGTTTCAGTATTT
  ACGACGCTCACCATTAATGGACGCCTGTTTGCTTGCCCGCGAGAGCAATTCGATTCACTGACTCCCTTAC
  CAGAACAGGAAGGCCCAACTGTTGGAACAGTGGGAACTTTTGAACTGATGAGCTCCAAAGATTTAGCATA
  CCAGATGACAATTTATGATTGGGAACTCTTCAACTGCGTGCATGAGCTGGAGCTAATCTATCACACATTT
  GGAAGGCATAATTTTAAAAAGACCACAGCAAACTTGGATTTGTTCCTGAGGAGATTTAATGAAATTCAGT
  TTTGGGTCGTCACTGAGATCTGCCTTTGTTCTCAGCTCAGCAAGCGTGTTCAGCTATTAAAAAAATTTAT
  TAAGATAGCAGCCCACTGTAAGGAGTATAAAAATCTGAATTCCTTTTTTGCCATCGTCATGGGACTAAGT
  AACGTTGCTGTGAGCCGCTTGGCACTAACGTGGGAGAAACTGCCAAGCAAGTTCAAGAAGTTCTATGCGG
  AGTTTGAAAGTTTAATGGACCCTTCAAGGAACCACAGGGCCTACAGGCTGACAGTAGCTAAGCTGGAACC
  TCCTCTCATCCCCTTCATGCCTTTGCTCATTAAAGATATGACATTTACTCATGAGGGGAACAAGACGTTC
  ATTGACAATCTAGTAAACTTTGAAAAAATGCGCATGATTGCAAATACGGCCAGAACAGTGAGATACTACA
  GGAGCCAACCCTTCAATCCTGATGCAGCTCAAGCTAATAAGAACCATCAGGATGTCCGGAGTTATGTACG
  GCAATTAAATGTGATTGACAACCAGAGAACTTTATCACAGATGTCACACAGATTAGAGCCTCGTCGACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239661 representing NM_001282900
  Red=Cloning site Green=Tags(s)
  
  MAGLLAPPYGVMETGSNNDRIPDKENVPSEKILRAGKILRNAILSRAPHMIRDRKYHLKTYRQCCVGTEL
  VDWMMQQTPCVHSRTQAVGMWQVLLEDGVLNHVDQEHHFQDKYLFYRFLDDEHEDAPLPTEEEKKECDEE
  LQDTMLLLSQMGPDAHMRMILRKPPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHAKGG
  TVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKE
  DFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQKYTVMSGTPEKILEHFLETIRLEAT
  LNEATDSVLNDFIMMHCVFMPNTQLCPALVAHYHAQPSQGTEQEKMDYALNNKRRVIRLVLQWAAMYGDL
  LQEDDVSMAFLEEFYVSVSDDARMIAALKEQLPELEKIVKQISEDAKAPQKKHKVLLQQFNTGDERAQKR
  QPIRGSDEVLFKVYCMDHTYTTIRVPVATSVKEVISAVADKLGSGEGLIIVKMSSGGEKVVLKPNDVSVF
  TTLTINGRLFACPREQFDSLTPLPEQEGPTVGTVGTFELMSSKDLAYQMTIYDWELFNCVHELELIYHTF
  GRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLS
  NVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRLTVAKLEPPLIPFMPLLIKDMTFTHEGNKTF
  IDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282900
• ORF Size: 2520 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001282900.1, NP_001269829.1
• RefSeq Size: 3937
• RefSeq ORF: 2523
• Locus ID: 11069
• Protein Pathways: Leukocyte transendothelial migration
• MW: 96.7 kDa