Epac2 (RAPGEF4) (NM_001282899) Human Tagged ORF Clone

CAT#: RC239694

RAPGEF4 (myc-DDK-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RAPGEF4
• Synonyms: CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2; Nbla00496
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239694 representing NM_001282899
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGACTTCTGGCTCCTCCTTATGGTGTTATGGAAACGGGCTCTAACAATGACAGGATTCCTGACA
  AGGAGAACACACCTCTCATTGAACCTCACGTTCCTCTTCGTCCTGCTAACACCATTACCAAGGTCCCTTC
  AGAGAAGATCCTCAGAGCTGGAAAAATTTTACGAAATGCCATTCTCTCTCGAGCACCTCACATGATAAGA
  GATAGAAAATACCACCTAAAGACATACAGACAATGCTGTGTGGGAACTGAACTGGTGGACTGGATGATGC
  AGCAGACACCATGTGTTCACTCCCGGACTCAAGCTGTTGGCATGTGGCAAGTCCTGTTAGAAGATGGTGT
  TCTCAACCACGTGGACCAGGAGCACCATTTCCAAGACAAATATTTATTCTATCGATTTCTGGATGATGAG
  CACGAGGATGCCCCTTTGCCTACTGAGGAGGAGAAGAAGGAGTGTGATGAGGAGCTCCAGGACACCATGC
  TGCTGCTGTCACAGATGGGCCCCGACGCCCACATGAGGATGATCCTTCGCAAACCACCTGGCCAGAGGAC
  TGTGGATGACCTAGAGATTATCTATGAGGAGCTTCTTCATATTAAAGCCTTATCCCATCTTTCTACCACA
  GTGAAACGAGAGTTAGCAGGTGTTCTCATTTTTGAGTCTCACGCCAAAGGAGGGACTGTGTTGTTTAACC
  AGGGGGAAGAAGGTACCTCCTGGTACATTATTCTAAAAGGATCAGTGAATGTAGTCATTTACGGCAAGGG
  TGTGGTCTGCACCCTGCATGAAGGAGATGACTTCGGCAAGTTAGCACTAGTGAATGATGCCCCACGAGCT
  GCCTCTATCGTCTTACGAGAAGATAACTGCCATTTCTTAAGAGTAGACAAGGAGGATTTCAACCGGATCC
  TAAGGGACGTGGAGGCGAATACAGTCAGACTTAAAGAACATGACCAAGATGTCTTGGTGCTGGAGAAGGT
  CCCAGCAGGGAACAGAGCTTCTAATCAAGGAAACTCACAGCCTCAGCAAAAGTATACTGTGATGTCAGGA
  ACACCTGAAAAAATTTTAGAGCATTTTCTAGAAACAATACGCCTTGAGGCAACTTTAAATGAAGCAACAG
  ATTCTGTTTTAAATGACTTTATTATGATGCACTGTGTTTTTATGCCAAATACCCAGCTTTGCCCGGCACT
  GGTGGCCCACTACCACGCACAGCCTTCACAAGGTACAGAACAGGAGAAAATGGATTATGCCCTCAACAAT
  AAGAGGCGAGTCATCCGCCTGGTTCTACAGTGGGCTGCCATGTATGGAGACCTCCTGCAAGAGGATGACG
  TGTCTATGGCCTTCCTGGAGGAGTTTTATGTATCTGTATCAGATGATGCCCGGATGATTGCTGCCCTCAA
  GGAGCAACTGCCAGAGTTGGAGAAGATTGTCAAGCAAATCTCAGAAGATGCAAAGGCACCACAAAAGAAG
  CACAAGGTTCTTTTGCAACAGTTCAATACGGGCGATGAGAGAGCCCAGAAGCGCCAGCCTATCCGCGGCT
  CTGATGAAGTTCTGTTTAAGGTCTATTGCATGGACCACACCTACACAACCATTCGGGTGCCAGTGGCCAC
  TTCGGTGAAGGAAGTCATCAGTGCAGTTGCCGACAAGCTGGGCTCCGGGGAGGGCCTGATCATAGTCAAG
  ATGAGTTCCGGAGGAGAAAAGGTGGTGCTCAAACCTAATGATGTTTCAGTATTTACGACGCTCACCATTA
  ATGGACGCCTGTTTGCTTGCCCGCGAGAGCAATTCGATTCACTGACTCCCTTACCAGAACAGGAAGGCCC
  AACTGTTGGAACAGTGGGAACTTTTGAACTGATGAGCTCCAAAGATTTAGCATACCAGATGACAATTTAT
  GATTGGGAACTCTTCAACTGCGTGCATGAGCTGGAGCTAATCTATCACACATTTGGAAGGCATAATTTTA
  AAAAGACCACAGCAAACTTGGATTTGTTCCTGAGGAGATTTAATGAAATTCAGTTTTGGGTCGTCACTGA
  GATCTGCCTTTGTTCTCAGCTCAGCAAGCGTGTTCAGCTATTAAAAAAATTTATTAAGATAGCAGCCCAC
  TGTAAGGAGTATAAAAATCTGAATTCCTTTTTTGCCATCGTCATGGGACTAAGTAACGTTGCTGTGAGCC
  GCTTGGCACTAACGTGGGAGAAACTGCCAAGCAAGTTCAAGAAGTTCTATGCGGAGTTTGAAAGTTTAAT
  GGACCCTTCAAGGAACCACAGGGCCTACAGGCTGACAGTAGCTAAGCTGGAACCTCCTCTCATCCCCTTC
  ATGCCTTTGCTCATTAAAGATATGACATTTACTCATGAGGGGAACAAGACGTTCATTGACAATCTAGTAA
  ACTTTGAAAAAATGCGCATGATTGCAAATACGGCCAGAACAGTGAGATACTACAGGAGCCAACCCTTCAA
  TCCTGATGCAGCTCAAGCTAATAAGAACCATCAGGATGTCCGGAGTTATGTACGGCAATTAAATGTGATT
  GACAACCAGAGAACTTTATCACAGATGTCACACAGATTAGAGCCTCGTCGACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239694 representing NM_001282899
  Red=Cloning site Green=Tags(s)
  
  MAGLLAPPYGVMETGSNNDRIPDKENTPLIEPHVPLRPANTITKVPSEKILRAGKILRNAILSRAPHMIR
  DRKYHLKTYRQCCVGTELVDWMMQQTPCVHSRTQAVGMWQVLLEDGVLNHVDQEHHFQDKYLFYRFLDDE
  HEDAPLPTEEEKKECDEELQDTMLLLSQMGPDAHMRMILRKPPGQRTVDDLEIIYEELLHIKALSHLSTT
  VKRELAGVLIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRA
  ASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQKYTVMSG
  TPEKILEHFLETIRLEATLNEATDSVLNDFIMMHCVFMPNTQLCPALVAHYHAQPSQGTEQEKMDYALNN
  KRRVIRLVLQWAAMYGDLLQEDDVSMAFLEEFYVSVSDDARMIAALKEQLPELEKIVKQISEDAKAPQKK
  HKVLLQQFNTGDERAQKRQPIRGSDEVLFKVYCMDHTYTTIRVPVATSVKEVISAVADKLGSGEGLIIVK
  MSSGGEKVVLKPNDVSVFTTLTINGRLFACPREQFDSLTPLPEQEGPTVGTVGTFELMSSKDLAYQMTIY
  DWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRVQLLKKFIKIAAH
  CKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRLTVAKLEPPLIPF
  MPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNHQDVRSYVRQLNVI
  DNQRTLSQMSHRLEPRRP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282899
• ORF Size: 2574 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001282899.1, NP_001269828.1
• RefSeq Size: 3991
• RefSeq ORF: 2577
• Locus ID: 11069
• Protein Pathways: Leukocyte transendothelial migration
• MW: 98.7 kDa