SALL2 (NM_001291447) Human Tagged ORF Clone

CAT#: RC239764

SALL2 (myc-DDK-tagged) - Human spalt-like transcription factor 2 (SALL2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SALL2
• Synonyms: COLB; HSAL2; p150(Sal2); Sal-2; ZNF795
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239764 representing NM_001291447
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCACGAATCCGAGAGGAGCTCTCGTCTCGGGGTGCCCTGCGGGGAGCCGGCAGAGCTCGGAGGTG
  ATGCTAGCGAGGAGGATCACCCCCAAGTCTGTGCCAAGTGCTGCGCACAATTCACTGACCCAACTGAATT
  CCTCGCCCACCAGAACGCATGTTCTACTGACCCTCCTGTAATGGTGATAATTGGGGGCCAGGAGAACCCC
  AACAACTCTTCGGCCTCCTCTGAACCCCGGCCTGAGGGTCACAATAATCCTCAGGTCATGGACACAGAGC
  ATAGCAACCCCCCAGATTCTGGGTCCTCCGTGCCCACGGATCCCACCTGGGGCCCAGAGAGGAGAGGAGA
  GGAGTCTTCAGGGCATTTCCTGGTCGCTGCCACAGGGGCAGAAACGCCCAAGCAGGCCTTCTTCCACCTT
  TACCACCCACTGGGGTCACAGCATCCTTTCTCTGCTGGAGGGGTTGGGCGAAGCCACAAACCCACCCCTG
  CCCCTTCCCCAGCCTTGCCAGGCAGCACAGATCAGCTGATTGCCTCGCCTCATCTGGCATTCCCAAGCAC
  CACGGGACTACTGGCAGCACAGTGTCTTGGGGCAGCCCGAGGCCTTGAGGCCACTGCCTCCCCAGGGCTC
  CTGAAGCCAAAGAATGGAAGTGGTGAGCTGAGCTACGGAGAAGTGATGGGTCCCTTGGAGAAGCCTGGTG
  GAAGGCACAAATGCCGCTTCTGTGCCAAAGTATTTGGCAGTGACAGTGCCCTGCAGATCCACCTTCGTTC
  CCACACGGGTGAGAGGCCCTATAAGTGCAATGTCTGTGGAAACCGTTTTACCACCCGTGGCAACCTCAAA
  GTGCATTTCCACCGGCATCGTGAGAAGTACCCACATGTGCAGATGAACCCACACCCAGTACCAGAGCACC
  TAGACTATGTCATTACCAGCAGTGGCTTGCCTTATGGTATGTCCGTGCCACCAGAGAAGGCCGAGGAGGA
  GGCAGCCACTCCAGGTGGAGGGGTTGAGCGCAAGCCTCTGGTGGCCTCCACAACAGCACTCAGTGCCACA
  GAGAGCCTGACTCTGCTCTCCACCAGTGCAGGCACAGCCACGGCTCCAGGACTCCCTGCTTTCAATAAGT
  TTGTGCTCATGAAAGCAGTGGAACCCAAGAATAAAGCTGATGAAAACACCCCCCCAGGGAGTGAGGGCTC
  AGCCATCAGTGGAGTGGCAGAAAGTAGCACGGCAACTCGCATGCAACTAAGTAAGTTGGTGACTTCACTA
  CCAAGCTGGGCACTGCTTACCAACCACTTCAAGTCCACTGGCAGCTTCCCCTTCCCCTATGTGCTAGAGC
  CCTTGGGGGCCTCACCCTCTGAGACATCAAAGCTGCAGCAACTGGTAGAAAAGATTGACCGGCAAGGAGC
  TGTGGCGGTGACCTCAGCTGCCTCAGGAGCCCCCACCACCTCTGCCCCTGCACCTTCATCCTCAGCCTCT
  TCTGGACCTAACCAGTGTGTCATCTGTCTCCGAGTGCTTAGCTGTCCTCGGGCCCTACGCCTTCATTATG
  GCCAACATGGAGGTGAGAGGCCCTTCAAATGCAAAGTGTGTGGCAGAGCCTTCTCCACCAGGGGTAATCT
  GCGTGCACATTTCGTGGGCCACAAGGCCAGTCCAGCTGCCCGGGCACAGAATTCCTGCCCCATCTGCCAG
  AAGAAGTTCACCAATGCTGTCACTCTGCAGCAGCATGTCCGGATGCACCTGGGGGGCCAGATCCCCAACG
  GTGGTACTGCACTCCCTGAAGGTGGAGGAGCTGCTCAGGAGAATGGCTCCGAGCAATCTACAGTCTCCGG
  GGCACGGAGTTTCCCCCAGCAGCAGTCCCAGCAGCCATCACCGGAAGAGGAGTTGTCTGAGGAGGAGGAA
  GAGGAGGATGAGGAAGAAGAGGAAGATGTGACTGATGAAGATTCCCTGGCAGGGAGAGGCTCAGAGAGTG
  GAGGTGAGAAGGCAATATCAGTGAGAGGTGATTCAGAAGAGGCATCTGGGGCAGAGGAGGAGGTGGGGAC
  AGTGGCGGCAGCAGCCACAGCTGGGAAGGAGATGGACAGTAATGAGAAAACTACTCAACAGTCTTCTTTG
  CCACCACCACCACCACCTGACAGCCTGGATCAGCCTCAGCCAATGGAGCAGGGAAGCAGTGGTGTTTTAG
  GAGGCAAGGAAGAGGGGGGCAAACCGGAGAGAAGCTCAAGTCCGGCATCAGCACTCACCCCAGAAGGGGA
  AGCCACCAGCGTGACCTTGGTAGAGGAGCTGAGCCTGCAGGAGGCAATGAGAAAGGAGCCAGGAGAGAGC
  AGCAGCAGAAAGGCCTGCGAAGTGTGTGGCCAGGCCTTTCCCTCCCAGGCAGCTCTGGAGGAGCATCAGA
  AGACCCACCCCAAGGAGGGGCCGCTCTTCACTTGTGTTTTCTGCAGGCAGGGCTTTCTTGAGCGGGCTAC
  CCTCAAGAAGCATATGCTCCTGGCACACCACCAGAACCAGTATGTGGCATTCCTGTCAAATGGCCTGCCC
  ATGAAGCCCTGGAATTCCAGCTCCACCTCCACTACCACTCCAAGCCTGGCCCCACCAGTGCTGTTTGGCC
  TAGGAACTGTGGCTGGGAAGGTGCCTCCAACAATGGGATCCAGGGAAGCCAAGGAGAAGACAGCCCCCCT
  CCTATTTCAGCCTCCTGCACCCAAGGCAGTGCCTGAGAAGCCCATCATAGACAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239764 representing NM_001291447
  Red=Cloning site Green=Tags(s)
  
  MAHESERSSRLGVPCGEPAELGGDASEEDHPQVCAKCCAQFTDPTEFLAHQNACSTDPPVMVIIGGQENP
  NNSSASSEPRPEGHNNPQVMDTEHSNPPDSGSSVPTDPTWGPERRGEESSGHFLVAATGAETPKQAFFHL
  YHPLGSQHPFSAGGVGRSHKPTPAPSPALPGSTDQLIASPHLAFPSTTGLLAAQCLGAARGLEATASPGL
  LKPKNGSGELSYGEVMGPLEKPGGRHKCRFCAKVFGSDSALQIHLRSHTGERPYKCNVCGNRFTTRGNLK
  VHFHRHREKYPHVQMNPHPVPEHLDYVITSSGLPYGMSVPPEKAEEEAATPGGGVERKPLVASTTALSAT
  ESLTLLSTSAGTATAPGLPAFNKFVLMKAVEPKNKADENTPPGSEGSAISGVAESSTATRMQLSKLVTSL
  PSWALLTNHFKSTGSFPFPYVLEPLGASPSETSKLQQLVEKIDRQGAVAVTSAASGAPTTSAPAPSSSAS
  SGPNQCVICLRVLSCPRALRLHYGQHGGERPFKCKVCGRAFSTRGNLRAHFVGHKASPAARAQNSCPICQ
  KKFTNAVTLQQHVRMHLGGQIPNGGTALPEGGGAAQENGSEQSTVSGARSFPQQQSQQPSPEEELSEEEE
  EEDEEEEEDVTDEDSLAGRGSESGGEKAISVRGDSEEASGAEEEVGTVAAAATAGKEMDSNEKTTQQSSL
  PPPPPPDSLDQPQPMEQGSSGVLGGKEEGGKPERSSSPASALTPEGEATSVTLVEELSLQEAMRKEPGES
  SSRKACEVCGQAFPSQAALEEHQKTHPKEGPLFTCVFCRQGFLERATLKKHMLLAHHQNQYVAFLSNGLP
  MKPWNSSSTSTTTPSLAPPVLFGLGTVAGKVPPTMGSREAKEKTAPLLFQPPAPKAVPEKPIIDKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291447
• ORF Size: 2718 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291447.1, NP_001278376.1
• RefSeq Size: 3948 bp
• RefSeq ORF: 2721 bp
• Locus ID: 6297
• Cytogenetics: 14q11.2
• Protein Families: Druggable Genome
• MW: 95.9 kDa
• Gene Summary: 'This gene encodes a protein containing multiple zinc finger domains. The encoded protein functions in optical fissure closure during development of the eye in the embryo. Mutations in this gene are associated with ocular coloboma. [provided by RefSeq, Jul 2016]'