GRIK5 (NM_001301030) Human Tagged ORF Clone

CAT#: RC239851

GRIK5 (myc-DDK-tagged) - Human glutamate receptor, ionotropic, kainate 5 (GRIK5), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRIK5
• Synonyms: EAA2; GluK5; GRIK2; KA2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239851 representing NM_001301030
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCTGAGCTGCTGCTGCTGCTGATTGTTGCCTTCGCCAGCCCCAGCTGCCAGGTGCTCTCATCAC
  TGCGCATGGCTGCAATCCTGGATGATCAGACAGTGTGTGGCCGCGGTGAGCGTCTGGCCTTGGCCTTGGC
  CCGGGAGCAGATCAACGGGATCATCGAGGTCCCAGCCAAGGCCCGAGTGGAAGTAGACATCTTTGAGCTG
  CAGCGGGACAGCCAGTACGAGACCACGGACACCATGTGTCAGATCTTACCCAAAGGGGTTGTGTCTGTCC
  TTGGGCCCTCCTCTAGCCCAGCATCTGCCTCCACCGTGAGCCATATCTGTGGAGAGAAGGAGATCCCCCA
  CATCAAGGTGGGTCCCGAGGAGACACCCCGCCTTCAGTACCTTCGCTTCGCGTCTGTCAGCCTGTACCCC
  AGTAACGAGGACGTCAGCTTGGCGGTCTCCCGAATCCTCAAGTCCTTCAACTACCCCTCGGCCAGCCTCA
  TCTGCGCCAAGGCTGAGTGCCTGCTGCGATTGGAGGAACTGGTGCGTGGCTTCCTCATCTCCAAGGAGAC
  GCTGTCAGTGAGGATGTTGGACGACAGCCGGGACCCCACACCACTGCTCAAGGAGATCCGTGATGACAAG
  GTGTCCACCATCATCATCGACGCCAACGCCTCCATCTCCCACCTCATCCTCCGTAAGGCCTCGGAACTGG
  GAATGACCTCAGCGTTTTACAAGTACATCCTCACCACCATGGACTTCCCCATCCTGCATCTGGACGGTAT
  TGTGGAGGACTCCTCCAACATCCTGGGCTTCTCCATGTTCAACACGTCCCACCCCTTCTACCCTGAGTTT
  GTCCGCAGCCTCAACATGTCCTGGAGGGAGAACTGTGAAGCCAGCACCTACCTGGGCCCTGCGCTGTCAG
  CCGCCCTGATGTTTGACGCCGTGCACGTGGTGGTGAGCGCTGTCCGAGAGCTGAACCGCAGCCAGGAGAT
  CGGTGTGAAGCCTCTGGCCTGTACATCGGCCAACATTTGGCCCCACGGGACCAGCCTCATGAACTACCTG
  CGCATGGTAGAGTATGATGGGCTGACCGGGCGGGTCGAGTTCAACAGCAAAGGGCAGAGAACCAACTACA
  CCCTGCGCATCCTAGAAAAGTCCCGGCAGGGCCACCGTGAGATTGGGGTGTGGTACTCTAACCGCACCCT
  GGCCATGAATGCCACCACCCTGGACATCAACCTGTCGCAGACACTGGCCAACAAGACCCTGGTGGTCACA
  ACCATCCTGGAGAACCCATACGTCATGCGCCGGCCCAACTTCCAGGCCCTGTCGGGGAACGAACGCTTCG
  AGGGCTTCTGCGTGGACATGCTGCGGGAGCTGGCCGAGCTGCTGCGCTTCCGCTACCGCCTGCGGTTGGT
  GGAGGATGGGCTGTACGGGGCGCCCGAGCCCAACGGCTCCTGGACGGGCATGGTTGGCGAGCTCATCAAC
  CGGAAGGCAGACCTGGCTGTGGCCGCCTTCACCATCACAGCTGAGCGGGAGAAGGTCATCGACTTTTCCA
  AGCCCTTTATGACCCTGGGGATCAGCATCCTCTACCGAGTGCACATGGGCCGCAAGCCTGGCTACTTCTC
  CTTCCTGGACCCCTTCTCCCCTGCTGTGTGGCTCTTCATGCTTCTTGCCTACCTGGCTGTCAGCTGCGTC
  CTGTTTCTGGCTGCCAGGCTGAGCCCCTATGAGTGGTATAACCCACACCCATGCCTGCGGGCACGCCCCC
  ACATCCTGGAGAACCAGTACACGCTGGGCAACAGCCTGTGGTTTCCCGTGGGGGGCTTCATGCAGCAGGG
  CTCGGAGATCATGCCCCGGGCGCTGTCCACGCGCTGTGTCAGCGGAGTCTGGTGGGCCTTCACCTTGATC
  ATCATCTCCTCCTACACGGCCAACCTGGCCGCCTTCCTCACCGTGCAGCGCATGGAGGTGCCTGTGGAGT
  CGGCCGATGACCTGGCAGATCAGACCAACATCGAGTATGGCACCATCCACGCCGGCTCCACCATGACCTT
  CTTCCAGAATTCACGGTACCAAACGTACCAGCGCATGTGGAACTACATGCAGTCGAAGCAGCCCAGCGTG
  TTCGTCAAGAGCACAGAAGAGGGCATTGCCCGCGTCCTCAACTCCCGCTACGCCTTCCTGCTCGAGTCCA
  CCATGAACGAATACCACCGGCGCCTCAACTGCAACCTCACCCAGATCGGGGGACTCCTCGACACCAAGGG
  CTACGGCATTGGCATGCCGCTGGGCTCCCCGTTCCGGGATGAGATCACACTGGCCATCCTGCAGCTTCAG
  GAGAACAACCGGCTGGAGATCCTGAAGCGCAAGTGGTGGGAGGGGGGCCGGTGCCCCAAGGAGGAGGACC
  ATCGAGCTAAAGGTTTGGGCATGGAGAACATTGGTGGCATTTTTATCGTGCTCATCTGTGGCCTCATCAT
  TGCTGTCTTCGTGGCGGTCATGGAATTCATATGGTCCACACGGAGGTCAGCTGAGTCCGAGGAGACCCCA
  GCTCTGCATCCAGCTGCCTGCCAGTGTTCTGCACTTGGCCCAAGGACTCCTCTGAAGGAGCCGTCCATGC
  TGCTAGTAAAGGTGCCCTCCACCCGTGTCCAGGTTGCCTTCTCAAGGACATCCCTCCGGCAAGTCTGTCC
  TTTCCTCCTGCAGCATCAGTTGTCCTCTCTTTATTGGATTCAGGCCACCAACGTACAAATATGCTGTCAT
  TTCTCCAGTCTTAAACCCTCTCCTGACCTCACTTTCCCTCCCAGCCACCGCCCACTTTCTTCCCTTCTCT
  TTACAGCCTTGGCAGCAGTGGGGGGTCTCCCTGATGCTTCCAGTTTCTTCTTTCCTCCCATTTCCTCTTG
  CCCCCCACTCCAGTCTGGCATCGGTCCCTGCCACTCCACTGAAGCCACTCTTGTCACCAGCAACTTCCAT
  GTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239851 representing NM_001301030
  Red=Cloning site Green=Tags(s)
  
  MPAELLLLLIVAFASPSCQVLSSLRMAAILDDQTVCGRGERLALALAREQINGIIEVPAKARVEVDIFEL
  QRDSQYETTDTMCQILPKGVVSVLGPSSSPASASTVSHICGEKEIPHIKVGPEETPRLQYLRFASVSLYP
  SNEDVSLAVSRILKSFNYPSASLICAKAECLLRLEELVRGFLISKETLSVRMLDDSRDPTPLLKEIRDDK
  VSTIIIDANASISHLILRKASELGMTSAFYKYILTTMDFPILHLDGIVEDSSNILGFSMFNTSHPFYPEF
  VRSLNMSWRENCEASTYLGPALSAALMFDAVHVVVSAVRELNRSQEIGVKPLACTSANIWPHGTSLMNYL
  RMVEYDGLTGRVEFNSKGQRTNYTLRILEKSRQGHREIGVWYSNRTLAMNATTLDINLSQTLANKTLVVT
  TILENPYVMRRPNFQALSGNERFEGFCVDMLRELAELLRFRYRLRLVEDGLYGAPEPNGSWTGMVGELIN
  RKADLAVAAFTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCV
  LFLAARLSPYEWYNPHPCLRARPHILENQYTLGNSLWFPVGGFMQQGSEIMPRALSTRCVSGVWWAFTLI
  IISSYTANLAAFLTVQRMEVPVESADDLADQTNIEYGTIHAGSTMTFFQNSRYQTYQRMWNYMQSKQPSV
  FVKSTEEGIARVLNSRYAFLLESTMNEYHRRLNCNLTQIGGLLDTKGYGIGMPLGSPFRDEITLAILQLQ
  ENNRLEILKRKWWEGGRCPKEEDHRAKGLGMENIGGIFIVLICGLIIAVFVAVMEFIWSTRRSAESEETP
  ALHPAACQCSALGPRTPLKEPSMLLVKVPSTRVQVAFSRTSLRQVCPFLLQHQLSSLYWIQATNVQICCH
  FSSLKPSPDLTFPPSHRPLSSLLFTALAAVGGLPDASSFFFPPISSCPPLQSGIGPCHSTEATLVTSNFH
  V
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301030
• ORF Size: 2943 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301030.1, NP_001287959.1
• RefSeq Size: 3331 bp
• RefSeq ORF: 2946 bp
• Locus ID: 2901
• Cytogenetics: 19q13.2
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 110.1 kDa
• Gene Summary: 'This gene encodes a protein that belongs to the glutamate-gated ionic channel family. Glutamate functions as the major excitatory neurotransmitter in the central nervous system through activation of ligand-gated ion channels and G protein-coupled membrane receptors. The protein encoded by this gene forms functional heteromeric kainate-preferring ionic channels with the subunits encoded by related gene family members. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]'